Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential

This study reports the electrochemical activities of a novel ion substituted-Hydroxyapatite (HAp) material in contact with HeLa cells. The work was performed to evaluate the inhibitory effects of various concentrations of HAp on ion transfer mechanisms in HeLa cells. The materials (n = 2: HAp1 and H...

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Autores principales:	Bernard Owusu Asimeng, Elvis Kwason Tiburu, Elsie Effah Kaufmann, Lily Paemka, Claude Fiifi Hayford, Samuel Essien-Baidoo, Obed Korshie Dzikunu, Prince Atsu Anani
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	cyclic voltammetry hydroxyapatite antiproliferation material Engineering (General). Civil engineering (General) TA1-2040
Acceso en línea:	https://doaj.org/article/198efcc0d1184df9934da3dd53791485
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spelling	oai:doaj.org-article:198efcc0d1184df9934da3dd537914852021-11-04T15:51:55ZElectrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential2331-191610.1080/23311916.2019.1614756https://doaj.org/article/198efcc0d1184df9934da3dd537914852019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/23311916.2019.1614756https://doaj.org/toc/2331-1916This study reports the electrochemical activities of a novel ion substituted-Hydroxyapatite (HAp) material in contact with HeLa cells. The work was performed to evaluate the inhibitory effects of various concentrations of HAp on ion transfer mechanisms in HeLa cells. The materials (n = 2: HAp1 and HAp3) were prepared at different stirring times from Achatina achatina snail shells and phosphate-containing solution. The structure of the materials and the trace elements concentration were evaluated using x-ray diffractometry and infrared spectrometry as well as atomic absorption spectroscopy. Electrochemical studies conducted on the cells after 30 min of exposure to the materials demonstrated different responses as elucidated by cyclic voltammetry. The voltammograms revealed HAp1 to be non-redox whereas HAp3 was redox active. Minimal concentrations of HAp1 showed high anodic peak current when compared to the HeLa cells alone, indicating a hyperpolarization of the cells. The peak current gradually reduced as the concentration of HAp1 was increased, and then followed by a sudden rise suggesting inhibition of the cell action potential. HAp3 showed a wavy pattern of the anodic peak current when the material concentration was varied. Peak currents of $$0.92 \pm 0.03 $$ nA and $$0.57 \pm 0.01$$ nA were recorded for HAp1 and HAp3, respectively at the highest concentration of 5 µL. The results suggest that different inhibitory mechanisms are at play on the voltage-gated ion channels of the cells, indicating the possibility of using the materials to achieve different cancer proliferation inhibition.Bernard Owusu AsimengElvis Kwason TiburuElsie Effah KaufmannLily PaemkaClaude Fiifi HayfordSamuel Essien-BaidooObed Korshie DzikunuPrince Atsu AnaniTaylor & Francis Grouparticlecyclic voltammetryhydroxyapatiteantiproliferation materialEngineering (General). Civil engineering (General)TA1-2040ENCogent Engineering, Vol 6, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyclic voltammetry hydroxyapatite antiproliferation material Engineering (General). Civil engineering (General) TA1-2040
spellingShingle	cyclic voltammetry hydroxyapatite antiproliferation material Engineering (General). Civil engineering (General) TA1-2040 Bernard Owusu Asimeng Elvis Kwason Tiburu Elsie Effah Kaufmann Lily Paemka Claude Fiifi Hayford Samuel Essien-Baidoo Obed Korshie Dzikunu Prince Atsu Anani Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential
description	This study reports the electrochemical activities of a novel ion substituted-Hydroxyapatite (HAp) material in contact with HeLa cells. The work was performed to evaluate the inhibitory effects of various concentrations of HAp on ion transfer mechanisms in HeLa cells. The materials (n = 2: HAp1 and HAp3) were prepared at different stirring times from Achatina achatina snail shells and phosphate-containing solution. The structure of the materials and the trace elements concentration were evaluated using x-ray diffractometry and infrared spectrometry as well as atomic absorption spectroscopy. Electrochemical studies conducted on the cells after 30 min of exposure to the materials demonstrated different responses as elucidated by cyclic voltammetry. The voltammograms revealed HAp1 to be non-redox whereas HAp3 was redox active. Minimal concentrations of HAp1 showed high anodic peak current when compared to the HeLa cells alone, indicating a hyperpolarization of the cells. The peak current gradually reduced as the concentration of HAp1 was increased, and then followed by a sudden rise suggesting inhibition of the cell action potential. HAp3 showed a wavy pattern of the anodic peak current when the material concentration was varied. Peak currents of $$0.92 \pm 0.03 $$ nA and $$0.57 \pm 0.01$$ nA were recorded for HAp1 and HAp3, respectively at the highest concentration of 5 µL. The results suggest that different inhibitory mechanisms are at play on the voltage-gated ion channels of the cells, indicating the possibility of using the materials to achieve different cancer proliferation inhibition.
format	article
author	Bernard Owusu Asimeng Elvis Kwason Tiburu Elsie Effah Kaufmann Lily Paemka Claude Fiifi Hayford Samuel Essien-Baidoo Obed Korshie Dzikunu Prince Atsu Anani
author_facet	Bernard Owusu Asimeng Elvis Kwason Tiburu Elsie Effah Kaufmann Lily Paemka Claude Fiifi Hayford Samuel Essien-Baidoo Obed Korshie Dzikunu Prince Atsu Anani
author_sort	Bernard Owusu Asimeng
title	Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential
title_short	Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential
title_full	Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential
title_fullStr	Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential
title_full_unstemmed	Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential
title_sort	electrochemical evaluation of ion substituted-hydroxyapatite on hela cells plasma membrane potential
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/198efcc0d1184df9934da3dd53791485
work_keys_str_mv	AT bernardowusuasimeng electrochemicalevaluationofionsubstitutedhydroxyapatiteonhelacellsplasmamembranepotential AT elviskwasontiburu electrochemicalevaluationofionsubstitutedhydroxyapatiteonhelacellsplasmamembranepotential AT elsieeffahkaufmann electrochemicalevaluationofionsubstitutedhydroxyapatiteonhelacellsplasmamembranepotential AT lilypaemka electrochemicalevaluationofionsubstitutedhydroxyapatiteonhelacellsplasmamembranepotential AT claudefiifihayford electrochemicalevaluationofionsubstitutedhydroxyapatiteonhelacellsplasmamembranepotential AT samuelessienbaidoo electrochemicalevaluationofionsubstitutedhydroxyapatiteonhelacellsplasmamembranepotential AT obedkorshiedzikunu electrochemicalevaluationofionsubstitutedhydroxyapatiteonhelacellsplasmamembranepotential AT princeatsuanani electrochemicalevaluationofionsubstitutedhydroxyapatiteonhelacellsplasmamembranepotential
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Electrochemical evaluation of ion substituted-hydroxyapatite on HeLa cells plasma membrane potential

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