Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging

Abstract Current in vivo neuroimaging techniques provide limited field of view or spatial resolution and often require exogenous contrast. These limitations prohibit detailed structural imaging across wide fields of view and hinder intraoperative tumor margin detection. Here we present a novel neuro...

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Autores principales:	Derek Yecies, Orly Liba, Elliott D. SoRelle, Rebecca Dutta, Edwin Yuan, Hannes Vogel, Gerald A. Grant, Adam de la Zerda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/199130ba38364d31b2e1f561ecf45af7
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spelling	oai:doaj.org-article:199130ba38364d31b2e1f561ecf45af72021-12-02T15:08:32ZSpeckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging10.1038/s41598-019-45902-92045-2322https://doaj.org/article/199130ba38364d31b2e1f561ecf45af72019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45902-9https://doaj.org/toc/2045-2322Abstract Current in vivo neuroimaging techniques provide limited field of view or spatial resolution and often require exogenous contrast. These limitations prohibit detailed structural imaging across wide fields of view and hinder intraoperative tumor margin detection. Here we present a novel neuroimaging technique, speckle-modulating optical coherence tomography (SM-OCT), which allows us to image the brains of live mice and ex vivo human samples with unprecedented resolution and wide field of view using only endogenous contrast. The increased visibility provided by speckle elimination reveals white matter fascicles and cortical layer architecture in brains of live mice. To our knowledge, the data reported herein represents the highest resolution imaging of murine white matter structure achieved in vivo across a wide field of view of several millimeters. When applied to an orthotopic murine glioblastoma xenograft model, SM-OCT readily identifies brain tumor margins with resolution of approximately 10 μm. SM-OCT of ex vivo human temporal lobe tissue reveals fine structures including cortical layers and myelinated axons. Finally, when applied to an ex vivo sample of a low-grade glioma resection margin, SM-OCT is able to resolve the brain tumor margin. Based on these findings, SM-OCT represents a novel approach for intraoperative tumor margin detection and in vivo neuroimaging.Derek YeciesOrly LibaElliott D. SoRelleRebecca DuttaEdwin YuanHannes VogelGerald A. GrantAdam de la ZerdaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Derek Yecies Orly Liba Elliott D. SoRelle Rebecca Dutta Edwin Yuan Hannes Vogel Gerald A. Grant Adam de la Zerda Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging
description	Abstract Current in vivo neuroimaging techniques provide limited field of view or spatial resolution and often require exogenous contrast. These limitations prohibit detailed structural imaging across wide fields of view and hinder intraoperative tumor margin detection. Here we present a novel neuroimaging technique, speckle-modulating optical coherence tomography (SM-OCT), which allows us to image the brains of live mice and ex vivo human samples with unprecedented resolution and wide field of view using only endogenous contrast. The increased visibility provided by speckle elimination reveals white matter fascicles and cortical layer architecture in brains of live mice. To our knowledge, the data reported herein represents the highest resolution imaging of murine white matter structure achieved in vivo across a wide field of view of several millimeters. When applied to an orthotopic murine glioblastoma xenograft model, SM-OCT readily identifies brain tumor margins with resolution of approximately 10 μm. SM-OCT of ex vivo human temporal lobe tissue reveals fine structures including cortical layers and myelinated axons. Finally, when applied to an ex vivo sample of a low-grade glioma resection margin, SM-OCT is able to resolve the brain tumor margin. Based on these findings, SM-OCT represents a novel approach for intraoperative tumor margin detection and in vivo neuroimaging.
format	article
author	Derek Yecies Orly Liba Elliott D. SoRelle Rebecca Dutta Edwin Yuan Hannes Vogel Gerald A. Grant Adam de la Zerda
author_facet	Derek Yecies Orly Liba Elliott D. SoRelle Rebecca Dutta Edwin Yuan Hannes Vogel Gerald A. Grant Adam de la Zerda
author_sort	Derek Yecies
title	Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging
title_short	Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging
title_full	Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging
title_fullStr	Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging
title_full_unstemmed	Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging
title_sort	speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/199130ba38364d31b2e1f561ecf45af7
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Speckle modulation enables high-resolution wide-field human brain tumor margin detection and in vivo murine neuroimaging

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