Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth

Abstract. Fatmawati U, Meryandini A, Nawangsih AA, Wahyudi AT. 2019. Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas 20: 2970-2977. Actinomycetes which colonized plant rhizosphere has a vital role in improving plant growth b...

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Autores principales:	UMI FATMAWATI, ANJA MERYANDINI, ABDJAD ASIH NAWANGSIH, Aris Tri Wahyudi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	actinomycetes, germination, iaa production, phosphate solubilizing, soybean rhizosphere Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/199d37d98b2e4a9d99192f663eedba3e
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spelling	oai:doaj.org-article:199d37d98b2e4a9d99192f663eedba3e2021-11-21T21:54:58ZScreening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth1412-033X2085-472210.13057/biodiv/d201027https://doaj.org/article/199d37d98b2e4a9d99192f663eedba3e2019-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4398https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Fatmawati U, Meryandini A, Nawangsih AA, Wahyudi AT. 2019. Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas 20: 2970-2977. Actinomycetes which colonized plant rhizosphere has a vital role in improving plant growth by producing Indole-3-Acetic Acid (IAA). The aim of this study was to evaluate the potency of actinomycetes isolated from soybean rhizosphere as promoter agents for plant growth in vitro. Fifty actinomycetes isolates were successfully isolated from soybean rhizosphere. Based on the colorimetric methods, 35 isolates can produce IAA in various concentration, in the range of 0.46-30.6 mg/L. Seed germination assay using Ragdoll methods revealed that 26 isolates significantly promoted germination parameters, including the hypocotyl and the radicular length, the number of the lateral roots, and dry weight of the plant. Also, 14 from 26 isolates showed phosphate solubilizing activity in different phosphate-solubilizing index ranging from 1.25-2.62. Eight isolates were able to grow in N-free medium, indicating that these isolates have the ability in fixing nitrogen. Out of 23 from 26 isolates were detected to produce siderophore. All the tested isolates show chitinase production except ASR 55. Based on the observed parameters, it showed that there are four potential isolates (ASR 46, ASR 58, ASR 75 and ASR 76) as promising plant-growth promoters, phosphate solubilizer, nitrogen fixer, siderophore and chitinase producer. Based on the result of 16S rRNA sequence analysis, four potential isolates were identified as Streptomyces spp. in different taxa of strains and species.UMI FATMAWATIANJA MERYANDINIABDJAD ASIH NAWANGSIHAris Tri WahyudiMBI & UNS Soloarticleactinomycetes, germination, iaa production, phosphate solubilizing, soybean rhizosphereBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	actinomycetes, germination, iaa production, phosphate solubilizing, soybean rhizosphere Biology (General) QH301-705.5
spellingShingle	actinomycetes, germination, iaa production, phosphate solubilizing, soybean rhizosphere Biology (General) QH301-705.5 UMI FATMAWATI ANJA MERYANDINI ABDJAD ASIH NAWANGSIH Aris Tri Wahyudi Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth
description	Abstract. Fatmawati U, Meryandini A, Nawangsih AA, Wahyudi AT. 2019. Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas 20: 2970-2977. Actinomycetes which colonized plant rhizosphere has a vital role in improving plant growth by producing Indole-3-Acetic Acid (IAA). The aim of this study was to evaluate the potency of actinomycetes isolated from soybean rhizosphere as promoter agents for plant growth in vitro. Fifty actinomycetes isolates were successfully isolated from soybean rhizosphere. Based on the colorimetric methods, 35 isolates can produce IAA in various concentration, in the range of 0.46-30.6 mg/L. Seed germination assay using Ragdoll methods revealed that 26 isolates significantly promoted germination parameters, including the hypocotyl and the radicular length, the number of the lateral roots, and dry weight of the plant. Also, 14 from 26 isolates showed phosphate solubilizing activity in different phosphate-solubilizing index ranging from 1.25-2.62. Eight isolates were able to grow in N-free medium, indicating that these isolates have the ability in fixing nitrogen. Out of 23 from 26 isolates were detected to produce siderophore. All the tested isolates show chitinase production except ASR 55. Based on the observed parameters, it showed that there are four potential isolates (ASR 46, ASR 58, ASR 75 and ASR 76) as promising plant-growth promoters, phosphate solubilizer, nitrogen fixer, siderophore and chitinase producer. Based on the result of 16S rRNA sequence analysis, four potential isolates were identified as Streptomyces spp. in different taxa of strains and species.
format	article
author	UMI FATMAWATI ANJA MERYANDINI ABDJAD ASIH NAWANGSIH Aris Tri Wahyudi
author_facet	UMI FATMAWATI ANJA MERYANDINI ABDJAD ASIH NAWANGSIH Aris Tri Wahyudi
author_sort	UMI FATMAWATI
title	Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth
title_short	Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth
title_full	Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth
title_fullStr	Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth
title_full_unstemmed	Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth
title_sort	screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/199d37d98b2e4a9d99192f663eedba3e
work_keys_str_mv	AT umifatmawati screeningandcharacterizationofactinomycetesisolatedfromsoybeanrhizosphereforpromotingplantgrowth AT anjameryandini screeningandcharacterizationofactinomycetesisolatedfromsoybeanrhizosphereforpromotingplantgrowth AT abdjadasihnawangsih screeningandcharacterizationofactinomycetesisolatedfromsoybeanrhizosphereforpromotingplantgrowth AT aristriwahyudi screeningandcharacterizationofactinomycetesisolatedfromsoybeanrhizosphereforpromotingplantgrowth
_version_	1718418627916464128

Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth

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