EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS

Objective: To study the effect of cisplatin administration on oxidative stress induced hepatotoxicity in male Sprague Dawley rats. Study Design: Randomized controlled trial. Place and Duration of Study: Study was conducted at department of Physiology, Army Medical College, Rawalpindi. Duration...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sajid Ali, Muhammad Alamgir Khan, Amina Rasul, nadia Latif, Kamil Asghar Imam, Sumayya Bashir
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2019
Materias:	cisplatin hepatotoxicity Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/199da79f86d443c9966fa0c19b9514f3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:199da79f86d443c9966fa0c19b9514f3
record_format	dspace
spelling	oai:doaj.org-article:199da79f86d443c9966fa0c19b9514f32021-11-12T02:39:16ZEVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS0030-96482411-8842https://doaj.org/article/199da79f86d443c9966fa0c19b9514f32019-06-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/3016/2254https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To study the effect of cisplatin administration on oxidative stress induced hepatotoxicity in male Sprague Dawley rats. Study Design: Randomized controlled trial. Place and Duration of Study: Study was conducted at department of Physiology, Army Medical College, Rawalpindi. Duration of study was 18 months from Oct 2014 to Apr 2016. Material and Methods: The trial was performed on sixty male Sprague Dawley rats which were distributed randomly into two groups of 30 rats each. Group I received placebo whereas group II received intraperitoneal cisplatin 2mg/kg body weight two times a week for the period of 4 weeks. After successful treatment, animals were sacrificed and terminal blood sample was collected and used for estimation of serum AST, ALT, albumin and 8-isoprostane. Dissection of rats were done and liver tissue sampled. Tissue homogenate was prepared from liver sample which was used for estimation of total glutathione levels. Results: In group I, 8-isoprostane was 18.31 ± 3.35 pg/ml, total glutathione was 4.29 ± 0.42 μmol/L, ALT was 36.93 ± 4.72 IU/L, AST was 124.2 ± 12.75 IU/L and Albumin was 4.11 ± 0.26 g/dl whereas in group II, 8-isoprostane was 67.9 ± 8.14 pg/ml, total glutathione was 1.92 ± 0.28 μmol/L, ALT was 87.17 ± 6.47 IU/L, AST was 357.7 ± 19.37 IU/L and Albumin was 2.12 ± 0.25 g/dl. Levels of serum 8-isoprostane, ALT and AST were significantly raised (p<0.001) whereas serum albumin and total glutathione in liver tissue were found significantly low (p<0.001) in group II as compared to group I. Conclusion: Cisplatin treatment causes hepatotoxicity by increased production of reactive oxygen species in male Sprague Dawley rats.Sajid AliMuhammad Alamgir KhanAmina Rasulnadia LatifKamil Asghar ImamSumayya BashirArmy Medical College RawalpindiarticlecisplatinhepatotoxicityMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 69, Iss 3, Pp 500-504 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cisplatin hepatotoxicity Medicine R Medicine (General) R5-920
spellingShingle	cisplatin hepatotoxicity Medicine R Medicine (General) R5-920 Sajid Ali Muhammad Alamgir Khan Amina Rasul nadia Latif Kamil Asghar Imam Sumayya Bashir EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS
description	Objective: To study the effect of cisplatin administration on oxidative stress induced hepatotoxicity in male Sprague Dawley rats. Study Design: Randomized controlled trial. Place and Duration of Study: Study was conducted at department of Physiology, Army Medical College, Rawalpindi. Duration of study was 18 months from Oct 2014 to Apr 2016. Material and Methods: The trial was performed on sixty male Sprague Dawley rats which were distributed randomly into two groups of 30 rats each. Group I received placebo whereas group II received intraperitoneal cisplatin 2mg/kg body weight two times a week for the period of 4 weeks. After successful treatment, animals were sacrificed and terminal blood sample was collected and used for estimation of serum AST, ALT, albumin and 8-isoprostane. Dissection of rats were done and liver tissue sampled. Tissue homogenate was prepared from liver sample which was used for estimation of total glutathione levels. Results: In group I, 8-isoprostane was 18.31 ± 3.35 pg/ml, total glutathione was 4.29 ± 0.42 μmol/L, ALT was 36.93 ± 4.72 IU/L, AST was 124.2 ± 12.75 IU/L and Albumin was 4.11 ± 0.26 g/dl whereas in group II, 8-isoprostane was 67.9 ± 8.14 pg/ml, total glutathione was 1.92 ± 0.28 μmol/L, ALT was 87.17 ± 6.47 IU/L, AST was 357.7 ± 19.37 IU/L and Albumin was 2.12 ± 0.25 g/dl. Levels of serum 8-isoprostane, ALT and AST were significantly raised (p<0.001) whereas serum albumin and total glutathione in liver tissue were found significantly low (p<0.001) in group II as compared to group I. Conclusion: Cisplatin treatment causes hepatotoxicity by increased production of reactive oxygen species in male Sprague Dawley rats.
format	article
author	Sajid Ali Muhammad Alamgir Khan Amina Rasul nadia Latif Kamil Asghar Imam Sumayya Bashir
author_facet	Sajid Ali Muhammad Alamgir Khan Amina Rasul nadia Latif Kamil Asghar Imam Sumayya Bashir
author_sort	Sajid Ali
title	EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS
title_short	EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS
title_full	EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS
title_fullStr	EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS
title_full_unstemmed	EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS
title_sort	evaluation of oxidative stress induced hepatotoxicity produced by cisplatin in male sprague dawley rats
publisher	Army Medical College Rawalpindi
publishDate	2019
url	https://doaj.org/article/199da79f86d443c9966fa0c19b9514f3
work_keys_str_mv	AT sajidali evaluationofoxidativestressinducedhepatotoxicityproducedbycisplatininmalespraguedawleyrats AT muhammadalamgirkhan evaluationofoxidativestressinducedhepatotoxicityproducedbycisplatininmalespraguedawleyrats AT aminarasul evaluationofoxidativestressinducedhepatotoxicityproducedbycisplatininmalespraguedawleyrats AT nadialatif evaluationofoxidativestressinducedhepatotoxicityproducedbycisplatininmalespraguedawleyrats AT kamilasgharimam evaluationofoxidativestressinducedhepatotoxicityproducedbycisplatininmalespraguedawleyrats AT sumayyabashir evaluationofoxidativestressinducedhepatotoxicityproducedbycisplatininmalespraguedawleyrats
_version_	1718431274555670528

EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS

Ejemplares similares