Characterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber

Long non-coding RNAs (lncRNAs) have been reported to play critical roles during pathogen infection and innate immune response in mammals. Such observation inspired us to explore the expression profiles and functions of lncRNAs in invertebrates upon bacterial infection. Here, the lncRNAs of sea cucum...

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Autores principales:	Siyuan Zhang, Yina Shao, Chenghua Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Apostichopus japonicas lncRNA Vibrio splendidus immune response miRNA sponge Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/19a132101ad248b0a74404dc98308be2
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id	oai:doaj.org-article:19a132101ad248b0a74404dc98308be2
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spelling	oai:doaj.org-article:19a132101ad248b0a74404dc98308be22021-11-16T07:00:36ZCharacterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber1664-322410.3389/fimmu.2021.792040https://doaj.org/article/19a132101ad248b0a74404dc98308be22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.792040/fullhttps://doaj.org/toc/1664-3224Long non-coding RNAs (lncRNAs) have been reported to play critical roles during pathogen infection and innate immune response in mammals. Such observation inspired us to explore the expression profiles and functions of lncRNAs in invertebrates upon bacterial infection. Here, the lncRNAs of sea cucumber (Apostichopus japonicus) involved in Vibrio splendidus infection were characterized. RNA-seq obtained 2897 differentially expressed lncRNAs from Vibrio splendidus infected coelomocytes of sea cucumbers. The potential functions of the significant differentially expressed lncRNAs were related to immunity and metabolic process based on the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Moreover, we identify a lncRNA (XLOC_028509), which is downregulated with Vibrio splendidus challenged, further study indicated that XLOC_028509 adsorb miR-2008 and miR-31 as competing endogenous RNAs (ceRNAs) through base complementarity, which in turn decreased the amount of miRNAs (microRNAs) bound to the 3’UTRs (untranslated regions) of mRNAs to reduce their inhibition of target gene translation. These data demonstrated that the lncRNAs of invertebrates might be important regulators in pathogen-host interactions by sponging miRNAs.Siyuan ZhangSiyuan ZhangYina ShaoYina ShaoChenghua LiChenghua LiChenghua LiFrontiers Media S.A.articleApostichopus japonicaslncRNAVibrio splendidusimmune responsemiRNA spongeImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apostichopus japonicas lncRNA Vibrio splendidus immune response miRNA sponge Immunologic diseases. Allergy RC581-607
spellingShingle	Apostichopus japonicas lncRNA Vibrio splendidus immune response miRNA sponge Immunologic diseases. Allergy RC581-607 Siyuan Zhang Siyuan Zhang Yina Shao Yina Shao Chenghua Li Chenghua Li Chenghua Li Characterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber
description	Long non-coding RNAs (lncRNAs) have been reported to play critical roles during pathogen infection and innate immune response in mammals. Such observation inspired us to explore the expression profiles and functions of lncRNAs in invertebrates upon bacterial infection. Here, the lncRNAs of sea cucumber (Apostichopus japonicus) involved in Vibrio splendidus infection were characterized. RNA-seq obtained 2897 differentially expressed lncRNAs from Vibrio splendidus infected coelomocytes of sea cucumbers. The potential functions of the significant differentially expressed lncRNAs were related to immunity and metabolic process based on the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Moreover, we identify a lncRNA (XLOC_028509), which is downregulated with Vibrio splendidus challenged, further study indicated that XLOC_028509 adsorb miR-2008 and miR-31 as competing endogenous RNAs (ceRNAs) through base complementarity, which in turn decreased the amount of miRNAs (microRNAs) bound to the 3’UTRs (untranslated regions) of mRNAs to reduce their inhibition of target gene translation. These data demonstrated that the lncRNAs of invertebrates might be important regulators in pathogen-host interactions by sponging miRNAs.
format	article
author	Siyuan Zhang Siyuan Zhang Yina Shao Yina Shao Chenghua Li Chenghua Li Chenghua Li
author_facet	Siyuan Zhang Siyuan Zhang Yina Shao Yina Shao Chenghua Li Chenghua Li Chenghua Li
author_sort	Siyuan Zhang
title	Characterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber
title_short	Characterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber
title_full	Characterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber
title_fullStr	Characterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber
title_full_unstemmed	Characterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber
title_sort	characterization of host lncrnas in response to vibrio splendidus infection and function as efficient mirna sponges in sea cucumber
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/19a132101ad248b0a74404dc98308be2
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Characterization of Host lncRNAs in Response to Vibrio splendidus Infection and Function as Efficient miRNA Sponges in Sea Cucumber

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