Molecular approach to the identification of fish in the South China Sea.

<h4>Background</h4>DNA barcoding is one means of establishing a rapid, accurate, and cost-effective system for the identification of species. It involves the use of short, standard gene targets to create sequence profiles of known species against sequences of unknowns that can be matched...

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Autores principales:	Junbin Zhang, Robert Hanner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19a227802965443797e4cc6a2a7bc7a1
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spelling	oai:doaj.org-article:19a227802965443797e4cc6a2a7bc7a12021-11-18T07:27:46ZMolecular approach to the identification of fish in the South China Sea.1932-620310.1371/journal.pone.0030621https://doaj.org/article/19a227802965443797e4cc6a2a7bc7a12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363454/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>DNA barcoding is one means of establishing a rapid, accurate, and cost-effective system for the identification of species. It involves the use of short, standard gene targets to create sequence profiles of known species against sequences of unknowns that can be matched and subsequently identified. The Fish Barcode of Life (FISH-BOL) campaign has the primary goal of gathering DNA barcode records for all the world's fish species. As a contribution to FISH-BOL, we examined the degree to which DNA barcoding can discriminate marine fishes from the South China Sea.<h4>Methodology/principal findings</h4>DNA barcodes of cytochrome oxidase subunit I (COI) were characterized using 1336 specimens that belong to 242 species fishes from the South China Sea. All specimen provenance data (including digital specimen images and geospatial coordinates of collection localities) and collateral sequence information were assembled using Barcode of Life Data System (BOLD; www.barcodinglife.org). Small intraspecific and large interspecific differences create distinct genetic boundaries among most species. In addition, the efficiency of two mitochondrial genes, 16S rRNA (16S) and cytochrome b (cytb), and one nuclear ribosomal gene, 18S rRNA (18S), was also evaluated for a few select groups of species.<h4>Conclusions/significance</h4>The present study provides evidence for the effectiveness of DNA barcoding as a tool for monitoring marine biodiversity. Open access data of fishes from the South China Sea can benefit relative applications in ecology and taxonomy.Junbin ZhangRobert HannerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e30621 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Junbin Zhang Robert Hanner Molecular approach to the identification of fish in the South China Sea.
description	<h4>Background</h4>DNA barcoding is one means of establishing a rapid, accurate, and cost-effective system for the identification of species. It involves the use of short, standard gene targets to create sequence profiles of known species against sequences of unknowns that can be matched and subsequently identified. The Fish Barcode of Life (FISH-BOL) campaign has the primary goal of gathering DNA barcode records for all the world's fish species. As a contribution to FISH-BOL, we examined the degree to which DNA barcoding can discriminate marine fishes from the South China Sea.<h4>Methodology/principal findings</h4>DNA barcodes of cytochrome oxidase subunit I (COI) were characterized using 1336 specimens that belong to 242 species fishes from the South China Sea. All specimen provenance data (including digital specimen images and geospatial coordinates of collection localities) and collateral sequence information were assembled using Barcode of Life Data System (BOLD; www.barcodinglife.org). Small intraspecific and large interspecific differences create distinct genetic boundaries among most species. In addition, the efficiency of two mitochondrial genes, 16S rRNA (16S) and cytochrome b (cytb), and one nuclear ribosomal gene, 18S rRNA (18S), was also evaluated for a few select groups of species.<h4>Conclusions/significance</h4>The present study provides evidence for the effectiveness of DNA barcoding as a tool for monitoring marine biodiversity. Open access data of fishes from the South China Sea can benefit relative applications in ecology and taxonomy.
format	article
author	Junbin Zhang Robert Hanner
author_facet	Junbin Zhang Robert Hanner
author_sort	Junbin Zhang
title	Molecular approach to the identification of fish in the South China Sea.
title_short	Molecular approach to the identification of fish in the South China Sea.
title_full	Molecular approach to the identification of fish in the South China Sea.
title_fullStr	Molecular approach to the identification of fish in the South China Sea.
title_full_unstemmed	Molecular approach to the identification of fish in the South China Sea.
title_sort	molecular approach to the identification of fish in the south china sea.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/19a227802965443797e4cc6a2a7bc7a1
work_keys_str_mv	AT junbinzhang molecularapproachtotheidentificationoffishinthesouthchinasea AT roberthanner molecularapproachtotheidentificationoffishinthesouthchinasea
_version_	1718423393505640448

Molecular approach to the identification of fish in the South China Sea.

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