Accessing depth-resolved high spatial frequency content from the optical coherence tomography signal

Abstract Optical coherence tomography (OCT) is a rapidly evolving technology with a broad range of applications, including biomedical imaging and diagnosis. Conventional intensity-based OCT provides depth-resolved imaging with a typical resolution and sensitivity to structural alterations of about 5...

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Autores principales:	Sergey Alexandrov, Anand Arangath, Yi Zhou, Mary Murphy, Niamh Duffy, Kai Neuhaus, Georgina Shaw, Ryan McAuley, Martin Leahy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19a61c8f3f8543f3bf6d9c6ed4d2176c
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spelling	oai:doaj.org-article:19a61c8f3f8543f3bf6d9c6ed4d2176c2021-12-02T15:09:16ZAccessing depth-resolved high spatial frequency content from the optical coherence tomography signal10.1038/s41598-021-96619-72045-2322https://doaj.org/article/19a61c8f3f8543f3bf6d9c6ed4d2176c2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96619-7https://doaj.org/toc/2045-2322Abstract Optical coherence tomography (OCT) is a rapidly evolving technology with a broad range of applications, including biomedical imaging and diagnosis. Conventional intensity-based OCT provides depth-resolved imaging with a typical resolution and sensitivity to structural alterations of about 5–10 microns. It would be desirable for functional biological imaging to detect smaller features in tissues due to the nature of pathological processes. In this article, we perform the analysis of the spatial frequency content of the OCT signal based on scattering theory. We demonstrate that the OCT signal, even at limited spectral bandwidth, contains information about high spatial frequencies present in the object which relates to the small, sub-wavelength size structures. Experimental single frame imaging of phantoms with well-known sub-micron internal structures confirms the theory. Examples of visualization of the nanoscale structural changes within mesenchymal stem cells (MSC), which are invisible using conventional OCT, are also shown. Presented results provide a theoretical and experimental basis for the extraction of high spatial frequency information to substantially improve the sensitivity of OCT to structural alterations at clinically relevant depths.Sergey AlexandrovAnand ArangathYi ZhouMary MurphyNiamh DuffyKai NeuhausGeorgina ShawRyan McAuleyMartin LeahyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sergey Alexandrov Anand Arangath Yi Zhou Mary Murphy Niamh Duffy Kai Neuhaus Georgina Shaw Ryan McAuley Martin Leahy Accessing depth-resolved high spatial frequency content from the optical coherence tomography signal
description	Abstract Optical coherence tomography (OCT) is a rapidly evolving technology with a broad range of applications, including biomedical imaging and diagnosis. Conventional intensity-based OCT provides depth-resolved imaging with a typical resolution and sensitivity to structural alterations of about 5–10 microns. It would be desirable for functional biological imaging to detect smaller features in tissues due to the nature of pathological processes. In this article, we perform the analysis of the spatial frequency content of the OCT signal based on scattering theory. We demonstrate that the OCT signal, even at limited spectral bandwidth, contains information about high spatial frequencies present in the object which relates to the small, sub-wavelength size structures. Experimental single frame imaging of phantoms with well-known sub-micron internal structures confirms the theory. Examples of visualization of the nanoscale structural changes within mesenchymal stem cells (MSC), which are invisible using conventional OCT, are also shown. Presented results provide a theoretical and experimental basis for the extraction of high spatial frequency information to substantially improve the sensitivity of OCT to structural alterations at clinically relevant depths.
format	article
author	Sergey Alexandrov Anand Arangath Yi Zhou Mary Murphy Niamh Duffy Kai Neuhaus Georgina Shaw Ryan McAuley Martin Leahy
author_facet	Sergey Alexandrov Anand Arangath Yi Zhou Mary Murphy Niamh Duffy Kai Neuhaus Georgina Shaw Ryan McAuley Martin Leahy
author_sort	Sergey Alexandrov
title	Accessing depth-resolved high spatial frequency content from the optical coherence tomography signal
title_short	Accessing depth-resolved high spatial frequency content from the optical coherence tomography signal
title_full	Accessing depth-resolved high spatial frequency content from the optical coherence tomography signal
title_fullStr	Accessing depth-resolved high spatial frequency content from the optical coherence tomography signal
title_full_unstemmed	Accessing depth-resolved high spatial frequency content from the optical coherence tomography signal
title_sort	accessing depth-resolved high spatial frequency content from the optical coherence tomography signal
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/19a61c8f3f8543f3bf6d9c6ed4d2176c
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Accessing depth-resolved high spatial frequency content from the optical coherence tomography signal

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