Preliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, Indonesia

Background and Aim: Coronavirus disease 2019 (COVID-19) is an acute infectious respiratory disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and has spread rapidly globally, resulting in a pandemic. In humans, the main routes of transmission are respiratory droplets and...

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Autores principales:	Hamong Suharsono, Ali Ghufron Mukti, Ketut Suryana, I. Wayan Masa Tenaya, Dilasdita Kartika Pradana, Guy Daly, Mochamad Panji Pujasakti
Formato:	article
Lenguaje:	EN
Publicado:	Veterinary World 2021
Materias:	pets severe acute respiratory syndrome coronavirus-2 viral detection Animal culture SF1-1100 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/19a93bfb9dc34d29ae798a382a988f30
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spelling	oai:doaj.org-article:19a93bfb9dc34d29ae798a382a988f302021-11-26T07:04:08ZPreliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, Indonesia10.14202/vetworld.2021.2979-29830972-89882231-0916https://doaj.org/article/19a93bfb9dc34d29ae798a382a988f302021-11-01T00:00:00Zhttp://www.veterinaryworld.org/Vol.14/November-2021/19.pdfhttps://doaj.org/toc/0972-8988https://doaj.org/toc/2231-0916Background and Aim: Coronavirus disease 2019 (COVID-19) is an acute infectious respiratory disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and has spread rapidly globally, resulting in a pandemic. In humans, the main routes of transmission are respiratory droplets and close contact with infected individuals or through contact with an object infected with the virus, followed by touching mouth, nose, or eyes. It is assumed that SARS-CoV-2 was originated in wild animals and was then transmitted to humans. Although some wildlife and domestic animals can be naturally or experimentally infected with the virus, the intermediate hosts that transmitted it to humans are still unknown. Understanding the dynamics of SARS-CoV-2 associated with possible zoonotic transmission of intermediate hosts is considered critical. Reportedly, cats or dogs living with COVID-19-positive humans tested positive for the disease, suggesting that the virus was transmitted to the animals from humans. Information regarding the epidemiological investigation and comprehensive studies is limited. Therefore, it is still unclear how high is the correlation of infection in humans and pet animals, especially those living together. The aim of this study was to investigate the possibility of SARS-CoV-2 infection in the pets of patients with COVID-19 who were hospitalized at the Wangaya hospital, Denpasar, Bali, Indonesia. Materials and Methods: A total of seven clinically asymptomatic pets (six dogs of different races and sexes and a cat [age, 360-2920 days]) were included in this study. These animals belonged to patients with confirmed SARS-CoV-2 infection from August to November 2020. Nasal swab and nasopharyngeal samples were collected from the pets individually under anesthetic condition and were collected 6-12 days after confirmed SARS-CoV-2 infection in owners and hospitalization at the Wangaya Hospital. The swab samples were then processed for RNA isolation and tested using reverse transcription-polymerase chain reaction (RT-PCR) for SARS-CoV-2, in accordance with the World Health Organization manual 2020. Results: RT-PCR results for all seven RNA samples, prepared from the swab samples, were negative. For the samples, all PCR products were below the threshold limit, suggesting no genetic material belonging to the samples tested. Conclusion: This was the first preliminary study of COVID-19 on pets in pandemic using RT-PCR. The study tested a very limited quantity of samples, and all of them were negative. However, the way in which the samples were prepared was considered appropriate. Therefore, in further studies, testing of more samples of pets of more individuals with confirmed SARS-CoV-2 infection is required.Hamong SuharsonoAli Ghufron MuktiKetut SuryanaI. Wayan Masa TenayaDilasdita Kartika PradanaGuy DalyMochamad Panji PujasaktiVeterinary Worldarticlepetssevere acute respiratory syndrome coronavirus-2viral detectionAnimal cultureSF1-1100Veterinary medicineSF600-1100ENVeterinary World, Vol 14, Iss 11, Pp 2979-2983 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pets severe acute respiratory syndrome coronavirus-2 viral detection Animal culture SF1-1100 Veterinary medicine SF600-1100
spellingShingle	pets severe acute respiratory syndrome coronavirus-2 viral detection Animal culture SF1-1100 Veterinary medicine SF600-1100 Hamong Suharsono Ali Ghufron Mukti Ketut Suryana I. Wayan Masa Tenaya Dilasdita Kartika Pradana Guy Daly Mochamad Panji Pujasakti Preliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, Indonesia
description	Background and Aim: Coronavirus disease 2019 (COVID-19) is an acute infectious respiratory disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and has spread rapidly globally, resulting in a pandemic. In humans, the main routes of transmission are respiratory droplets and close contact with infected individuals or through contact with an object infected with the virus, followed by touching mouth, nose, or eyes. It is assumed that SARS-CoV-2 was originated in wild animals and was then transmitted to humans. Although some wildlife and domestic animals can be naturally or experimentally infected with the virus, the intermediate hosts that transmitted it to humans are still unknown. Understanding the dynamics of SARS-CoV-2 associated with possible zoonotic transmission of intermediate hosts is considered critical. Reportedly, cats or dogs living with COVID-19-positive humans tested positive for the disease, suggesting that the virus was transmitted to the animals from humans. Information regarding the epidemiological investigation and comprehensive studies is limited. Therefore, it is still unclear how high is the correlation of infection in humans and pet animals, especially those living together. The aim of this study was to investigate the possibility of SARS-CoV-2 infection in the pets of patients with COVID-19 who were hospitalized at the Wangaya hospital, Denpasar, Bali, Indonesia. Materials and Methods: A total of seven clinically asymptomatic pets (six dogs of different races and sexes and a cat [age, 360-2920 days]) were included in this study. These animals belonged to patients with confirmed SARS-CoV-2 infection from August to November 2020. Nasal swab and nasopharyngeal samples were collected from the pets individually under anesthetic condition and were collected 6-12 days after confirmed SARS-CoV-2 infection in owners and hospitalization at the Wangaya Hospital. The swab samples were then processed for RNA isolation and tested using reverse transcription-polymerase chain reaction (RT-PCR) for SARS-CoV-2, in accordance with the World Health Organization manual 2020. Results: RT-PCR results for all seven RNA samples, prepared from the swab samples, were negative. For the samples, all PCR products were below the threshold limit, suggesting no genetic material belonging to the samples tested. Conclusion: This was the first preliminary study of COVID-19 on pets in pandemic using RT-PCR. The study tested a very limited quantity of samples, and all of them were negative. However, the way in which the samples were prepared was considered appropriate. Therefore, in further studies, testing of more samples of pets of more individuals with confirmed SARS-CoV-2 infection is required.
format	article
author	Hamong Suharsono Ali Ghufron Mukti Ketut Suryana I. Wayan Masa Tenaya Dilasdita Kartika Pradana Guy Daly Mochamad Panji Pujasakti
author_facet	Hamong Suharsono Ali Ghufron Mukti Ketut Suryana I. Wayan Masa Tenaya Dilasdita Kartika Pradana Guy Daly Mochamad Panji Pujasakti
author_sort	Hamong Suharsono
title	Preliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, Indonesia
title_short	Preliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, Indonesia
title_full	Preliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, Indonesia
title_fullStr	Preliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, Indonesia
title_full_unstemmed	Preliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, Indonesia
title_sort	preliminary study of coronavirus disease 2019 on pets in pandemic in denpasar, bali, indonesia
publisher	Veterinary World
publishDate	2021
url	https://doaj.org/article/19a93bfb9dc34d29ae798a382a988f30
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Preliminary study of coronavirus disease 2019 on pets in pandemic in Denpasar, Bali, Indonesia

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