Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.

Tuberculosis (TB), a contagious disease mainly caused by Mycobacterium tuberculosis (M. tb), Mycobacterium bovis (M. bovis), and Mycobacterium caprae (M. caprae), poses a major global threat to the health of humans and many species of animals. Developing an ante-mortem detection technique for differ...

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Autores principales:	Liang Sun, Yingyu Chen, Ping Yi, Li Yang, Yu Yan, Kailun Zhang, Qiaoying Zeng, Aizhen Guo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19aa318c7699477b97f67f77cdac7bc3
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spelling	oai:doaj.org-article:19aa318c7699477b97f67f77cdac7bc32021-12-02T20:17:15ZSerological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.1932-620310.1371/journal.pone.0257920https://doaj.org/article/19aa318c7699477b97f67f77cdac7bc32021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257920https://doaj.org/toc/1932-6203Tuberculosis (TB), a contagious disease mainly caused by Mycobacterium tuberculosis (M. tb), Mycobacterium bovis (M. bovis), and Mycobacterium caprae (M. caprae), poses a major global threat to the health of humans and many species of animals. Developing an ante-mortem detection technique for different species would be of significance in improving the surveillance employing a One Health strategy. To achieve this goal, a universal indirect ELISA was established for serologically detecting Mycobacterium tuberculosis complex infection for multiple live hosts by using a fusion protein of MPB70, MPB83, ESAT6, and CFP10 common in M. tb, M. bovis, and M. caprae as the coating antigen (MMEC) and HRP-labeled fusion protein A and G as a secondary antibody. After testing the known positive and negative sera, the receiver operating characteristic curves were constructed to decide the cut-off values. Then, the diagnostic sensitivity and specificity of MMEC/AG-iELISA were determined as 100.00% (95% CI: 96.90%, 100.00%) and 100.00% (95% CI: 98.44%, 100.00%) for M. bovis infection of cattle, 100.00% (95% CI: 95.00%, 100.00%) and 100.0% (95% CI: 96.80%, 100.00%) for M. bovis infection of sheep, 90.74% (95% CI: 80.09%, 95.98%) and 98.63% (95% CI: 95.14%, 99.76%) for M. bovis infection of cervids, 100.00% (95% CI: 15.81%, 100.00%) and 98.81% (95% CI: 93.54%, 99.97%) for M. bovis infection of monkeys, 100.00% (95% CI: 86.82%, 100.00%) and 94.85% (95% CI: 91.22%, 97.03%) for M. tb infection of humans. Furthermore, this MMEC/AG-iELISA likely detects M. caprae infection in roe deer. Thus this method has a promising application in serological TB surveillance for multiple animal species thereby providing evidence for taking further action in TB control.Liang SunYingyu ChenPing YiLi YangYu YanKailun ZhangQiaoying ZengAizhen GuoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0257920 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Liang Sun Yingyu Chen Ping Yi Li Yang Yu Yan Kailun Zhang Qiaoying Zeng Aizhen Guo Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.
description	Tuberculosis (TB), a contagious disease mainly caused by Mycobacterium tuberculosis (M. tb), Mycobacterium bovis (M. bovis), and Mycobacterium caprae (M. caprae), poses a major global threat to the health of humans and many species of animals. Developing an ante-mortem detection technique for different species would be of significance in improving the surveillance employing a One Health strategy. To achieve this goal, a universal indirect ELISA was established for serologically detecting Mycobacterium tuberculosis complex infection for multiple live hosts by using a fusion protein of MPB70, MPB83, ESAT6, and CFP10 common in M. tb, M. bovis, and M. caprae as the coating antigen (MMEC) and HRP-labeled fusion protein A and G as a secondary antibody. After testing the known positive and negative sera, the receiver operating characteristic curves were constructed to decide the cut-off values. Then, the diagnostic sensitivity and specificity of MMEC/AG-iELISA were determined as 100.00% (95% CI: 96.90%, 100.00%) and 100.00% (95% CI: 98.44%, 100.00%) for M. bovis infection of cattle, 100.00% (95% CI: 95.00%, 100.00%) and 100.0% (95% CI: 96.80%, 100.00%) for M. bovis infection of sheep, 90.74% (95% CI: 80.09%, 95.98%) and 98.63% (95% CI: 95.14%, 99.76%) for M. bovis infection of cervids, 100.00% (95% CI: 15.81%, 100.00%) and 98.81% (95% CI: 93.54%, 99.97%) for M. bovis infection of monkeys, 100.00% (95% CI: 86.82%, 100.00%) and 94.85% (95% CI: 91.22%, 97.03%) for M. tb infection of humans. Furthermore, this MMEC/AG-iELISA likely detects M. caprae infection in roe deer. Thus this method has a promising application in serological TB surveillance for multiple animal species thereby providing evidence for taking further action in TB control.
format	article
author	Liang Sun Yingyu Chen Ping Yi Li Yang Yu Yan Kailun Zhang Qiaoying Zeng Aizhen Guo
author_facet	Liang Sun Yingyu Chen Ping Yi Li Yang Yu Yan Kailun Zhang Qiaoying Zeng Aizhen Guo
author_sort	Liang Sun
title	Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.
title_short	Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.
title_full	Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.
title_fullStr	Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.
title_full_unstemmed	Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.
title_sort	serological detection of mycobacterium tuberculosis complex infection in multiple hosts by one universal elisa.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/19aa318c7699477b97f67f77cdac7bc3
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Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.

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