Characterization of M Cells in Tear Duct-Associated Lymphoid Tissue of Mice: A Potential Role in Immunosurveillance on the Ocular Surface

The ocular mucosal tissues are exposed to potentially harmful foreign antigens in the air and tear fluid. The tear duct-associated lymphoid tissue (TALT) may contribute to immune surveillance in the eye region. Follicle-associated epithelium (FAE) of TALTs is classified as stratified squamous epithe...

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Autores principales:	Yuki Oya, Shunsuke Kimura, Yutaka Nakamura, Narumi Ishihara, Shunsuke Takano, Ryo Morita, Mayumi Endo, Koji Hase
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	tear duct-associated lymphoid tissue microfold cell RANKL osteoprotegerin IgA Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/19ab8026053f41499e14a1e6ffaf4d9a
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spelling	oai:doaj.org-article:19ab8026053f41499e14a1e6ffaf4d9a2021-11-22T06:30:04ZCharacterization of M Cells in Tear Duct-Associated Lymphoid Tissue of Mice: A Potential Role in Immunosurveillance on the Ocular Surface1664-322410.3389/fimmu.2021.779709https://doaj.org/article/19ab8026053f41499e14a1e6ffaf4d9a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.779709/fullhttps://doaj.org/toc/1664-3224The ocular mucosal tissues are exposed to potentially harmful foreign antigens in the air and tear fluid. The tear duct-associated lymphoid tissue (TALT) may contribute to immune surveillance in the eye region. Follicle-associated epithelium (FAE) of TALTs is classified as stratified squamous epithelium and consists of squamous epithelial cells arranged in layers on the basement membrane. In contrast, most mucosa-associated lymphoid tissue is covered by a monolayer of epithelium containing microfold (M) cells. Therefore, antigen uptake and the presence of M cells in TALT are not fully understood. The present study found that a small population of FAE cells in the TALT expressed intestinal M-cell markers, namely Sox8, Tnfaip2, GP2, and OPG. This cell population was identified as functional M cells because of their uptake capacity of luminal nanoparticles. In addition, RANKL, which is essential for M-cell differentiation, was expressed by stroma-like cells at the subepithelial region and its receptor RANK by the FAE in the TALT. The administration of RANKL markedly increased the number of Sox8+ M cells. In contrast, deficiency in OPG, an endogenous inhibitor of RANKL, increased the number of M cells in the TALT. These data demonstrate that the RANKL-RANK axis is essential for M-cell differentiation in the TALT. Furthermore, immunization via eye drops elicited the production of antigen-specific antibodies in tears, which was enhanced by RANKL administration. Thus, TALT M cells play an important role in the immunosurveillance of the eye region.Yuki OyaShunsuke KimuraShunsuke KimuraYutaka NakamuraNarumi IshiharaShunsuke TakanoRyo MoritaMayumi EndoKoji HaseKoji HaseFrontiers Media S.A.articletear duct-associated lymphoid tissuemicrofold cellRANKLosteoprotegerinIgAImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tear duct-associated lymphoid tissue microfold cell RANKL osteoprotegerin IgA Immunologic diseases. Allergy RC581-607
spellingShingle	tear duct-associated lymphoid tissue microfold cell RANKL osteoprotegerin IgA Immunologic diseases. Allergy RC581-607 Yuki Oya Shunsuke Kimura Shunsuke Kimura Yutaka Nakamura Narumi Ishihara Shunsuke Takano Ryo Morita Mayumi Endo Koji Hase Koji Hase Characterization of M Cells in Tear Duct-Associated Lymphoid Tissue of Mice: A Potential Role in Immunosurveillance on the Ocular Surface
description	The ocular mucosal tissues are exposed to potentially harmful foreign antigens in the air and tear fluid. The tear duct-associated lymphoid tissue (TALT) may contribute to immune surveillance in the eye region. Follicle-associated epithelium (FAE) of TALTs is classified as stratified squamous epithelium and consists of squamous epithelial cells arranged in layers on the basement membrane. In contrast, most mucosa-associated lymphoid tissue is covered by a monolayer of epithelium containing microfold (M) cells. Therefore, antigen uptake and the presence of M cells in TALT are not fully understood. The present study found that a small population of FAE cells in the TALT expressed intestinal M-cell markers, namely Sox8, Tnfaip2, GP2, and OPG. This cell population was identified as functional M cells because of their uptake capacity of luminal nanoparticles. In addition, RANKL, which is essential for M-cell differentiation, was expressed by stroma-like cells at the subepithelial region and its receptor RANK by the FAE in the TALT. The administration of RANKL markedly increased the number of Sox8+ M cells. In contrast, deficiency in OPG, an endogenous inhibitor of RANKL, increased the number of M cells in the TALT. These data demonstrate that the RANKL-RANK axis is essential for M-cell differentiation in the TALT. Furthermore, immunization via eye drops elicited the production of antigen-specific antibodies in tears, which was enhanced by RANKL administration. Thus, TALT M cells play an important role in the immunosurveillance of the eye region.
format	article
author	Yuki Oya Shunsuke Kimura Shunsuke Kimura Yutaka Nakamura Narumi Ishihara Shunsuke Takano Ryo Morita Mayumi Endo Koji Hase Koji Hase
author_facet	Yuki Oya Shunsuke Kimura Shunsuke Kimura Yutaka Nakamura Narumi Ishihara Shunsuke Takano Ryo Morita Mayumi Endo Koji Hase Koji Hase
author_sort	Yuki Oya
title	Characterization of M Cells in Tear Duct-Associated Lymphoid Tissue of Mice: A Potential Role in Immunosurveillance on the Ocular Surface
title_short	Characterization of M Cells in Tear Duct-Associated Lymphoid Tissue of Mice: A Potential Role in Immunosurveillance on the Ocular Surface
title_full	Characterization of M Cells in Tear Duct-Associated Lymphoid Tissue of Mice: A Potential Role in Immunosurveillance on the Ocular Surface
title_fullStr	Characterization of M Cells in Tear Duct-Associated Lymphoid Tissue of Mice: A Potential Role in Immunosurveillance on the Ocular Surface
title_full_unstemmed	Characterization of M Cells in Tear Duct-Associated Lymphoid Tissue of Mice: A Potential Role in Immunosurveillance on the Ocular Surface
title_sort	characterization of m cells in tear duct-associated lymphoid tissue of mice: a potential role in immunosurveillance on the ocular surface
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/19ab8026053f41499e14a1e6ffaf4d9a
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Characterization of M Cells in Tear Duct-Associated Lymphoid Tissue of Mice: A Potential Role in Immunosurveillance on the Ocular Surface

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