Transcriptome analysis of arterial and venous circulating miRNAs during hypertension

Abstract Most current circulating miRNA biomarkers are derived from peripheral venous blood, whereas miRNA deregulation in arterial blood in disease conditions has been largely ignored. To explore whether peripheral venous blood miRNAs could represent a bona fide specific miRNA deregulation pattern,...

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Autores principales:	Ling Jin, Min Li, Hao Wang, Zhongnan Yin, Li Chen, Yang Zhou, Yongzheng Han, Qinghua Cui, Yuan Zhou, Lixiang Xue
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19ac83dd3fdb46f58894e1e304aa7140
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spelling	oai:doaj.org-article:19ac83dd3fdb46f58894e1e304aa71402021-12-02T14:11:32ZTranscriptome analysis of arterial and venous circulating miRNAs during hypertension10.1038/s41598-021-82979-72045-2322https://doaj.org/article/19ac83dd3fdb46f58894e1e304aa71402021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82979-7https://doaj.org/toc/2045-2322Abstract Most current circulating miRNA biomarkers are derived from peripheral venous blood, whereas miRNA deregulation in arterial blood in disease conditions has been largely ignored. To explore whether peripheral venous blood miRNAs could represent a bona fide specific miRNA deregulation pattern, we selected hypertension, a disease that is particularly associated with vessels, as the model. Circulating miRNA profiles of arterial and venous blood from spontaneously hypertensive (SHR) rats and their corresponding controls (i.e., WKY rats) were investigated by next-generation miRNA sequencing. Little miRNAs were observed between arterial and venous circulating miRNAs in WKY rats. Interestingly, this number was enhanced in SHR hypertensive rats. Bioinformatical analysis of disease association, enriched target genes and the regulatory transcription factors of these differentially expressed miRNAs implied a potential functional link with cardiovascular disease-related functions. Comparisons between arterial and venous miRNAs in hypertension-versus-control conditions also revealed prominent disease association of circulating miRNAs and their target genes in arteries but not in veins. Moreover, a young non-hypertensive animal model in SHR background (i.e. JSHR) was used as a second control for SHR. Additional transcriptomic analysis and droplet digital PCR validation of arterial and venous deregulated miRNAs among SHR and its two controls (WKY, JSHR) revealed a noticeable consensus of artery-deregulated miRNAs in hypertension and two novel arterial circulating signatures (miR-455-3p and miR-140-3p) of hypertension. These results suggest the necessity of re-evaluating the efficacy of certain venous miRNAs identified in previous studies as potential biomarkers in cardiovascular diseases or a wider disease spectrum.Ling JinMin LiHao WangZhongnan YinLi ChenYang ZhouYongzheng HanQinghua CuiYuan ZhouLixiang XueNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ling Jin Min Li Hao Wang Zhongnan Yin Li Chen Yang Zhou Yongzheng Han Qinghua Cui Yuan Zhou Lixiang Xue Transcriptome analysis of arterial and venous circulating miRNAs during hypertension
description	Abstract Most current circulating miRNA biomarkers are derived from peripheral venous blood, whereas miRNA deregulation in arterial blood in disease conditions has been largely ignored. To explore whether peripheral venous blood miRNAs could represent a bona fide specific miRNA deregulation pattern, we selected hypertension, a disease that is particularly associated with vessels, as the model. Circulating miRNA profiles of arterial and venous blood from spontaneously hypertensive (SHR) rats and their corresponding controls (i.e., WKY rats) were investigated by next-generation miRNA sequencing. Little miRNAs were observed between arterial and venous circulating miRNAs in WKY rats. Interestingly, this number was enhanced in SHR hypertensive rats. Bioinformatical analysis of disease association, enriched target genes and the regulatory transcription factors of these differentially expressed miRNAs implied a potential functional link with cardiovascular disease-related functions. Comparisons between arterial and venous miRNAs in hypertension-versus-control conditions also revealed prominent disease association of circulating miRNAs and their target genes in arteries but not in veins. Moreover, a young non-hypertensive animal model in SHR background (i.e. JSHR) was used as a second control for SHR. Additional transcriptomic analysis and droplet digital PCR validation of arterial and venous deregulated miRNAs among SHR and its two controls (WKY, JSHR) revealed a noticeable consensus of artery-deregulated miRNAs in hypertension and two novel arterial circulating signatures (miR-455-3p and miR-140-3p) of hypertension. These results suggest the necessity of re-evaluating the efficacy of certain venous miRNAs identified in previous studies as potential biomarkers in cardiovascular diseases or a wider disease spectrum.
format	article
author	Ling Jin Min Li Hao Wang Zhongnan Yin Li Chen Yang Zhou Yongzheng Han Qinghua Cui Yuan Zhou Lixiang Xue
author_facet	Ling Jin Min Li Hao Wang Zhongnan Yin Li Chen Yang Zhou Yongzheng Han Qinghua Cui Yuan Zhou Lixiang Xue
author_sort	Ling Jin
title	Transcriptome analysis of arterial and venous circulating miRNAs during hypertension
title_short	Transcriptome analysis of arterial and venous circulating miRNAs during hypertension
title_full	Transcriptome analysis of arterial and venous circulating miRNAs during hypertension
title_fullStr	Transcriptome analysis of arterial and venous circulating miRNAs during hypertension
title_full_unstemmed	Transcriptome analysis of arterial and venous circulating miRNAs during hypertension
title_sort	transcriptome analysis of arterial and venous circulating mirnas during hypertension
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/19ac83dd3fdb46f58894e1e304aa7140
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Transcriptome analysis of arterial and venous circulating miRNAs during hypertension

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