Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells

High-density lipoproteins (HDLs) play an important role in reverse cholesterol transport and present antioxidant properties, among others. In the central nervous system (CNS), there are HDLs, where these lipoproteins could influence brain health. Owing to the new evidence of HDL functionality remode...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elena Grao-Cruces, Maria C. Millan-Linares, Maria E. Martin-Rubio, Rocio Toscano, Sergio Barrientos-Trigo, Beatriz Bermudez, Sergio Montserrat-de la Paz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	obesity paraoxonase lipoproteins oxidation microglia neuroinflammation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/19ae9c854b2c4aee9db4f89175a2945e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:19ae9c854b2c4aee9db4f89175a2945e
record_format	dspace
spelling	oai:doaj.org-article:19ae9c854b2c4aee9db4f89175a2945e2021-11-25T16:51:20ZObesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells10.3390/biomedicines91117222227-9059https://doaj.org/article/19ae9c854b2c4aee9db4f89175a2945e2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1722https://doaj.org/toc/2227-9059High-density lipoproteins (HDLs) play an important role in reverse cholesterol transport and present antioxidant properties, among others. In the central nervous system (CNS), there are HDLs, where these lipoproteins could influence brain health. Owing to the new evidence of HDL functionality remodeling in obese patients, and the fact that obesity-associated metabolic disturbances is pro-inflammatory and pro-oxidant, the aim of this study was to investigate if HDL functions are depleted in obese patients and obesity-associated microenvironment. HDLs were isolated from normal-weight healthy (nwHDL) and obese men (obHDL). The oxHDL level was measured by malondialdehyde and 4-hydroxynoneal peroxided products. BV2 microglial cells were exposed to different concentrations of nwHDL and obHDL in different obesity-associated pro-inflammatory microenvironments. Our results showed that hyperleptinemia increased oxHDL levels. In addition, nwHDLs reduced pro-inflammatory cytokines’ release and M1 marker gene expression in BV2 microglial cells. Nevertheless, both nwHDL co-administered with LPS+leptin and obHDL promoted BV2 microglial activation and a higher pro-inflammatory cytokine production, thus confirming that obesity-associated metabolic disturbances reverse the antioxidant and anti-inflammatory properties of HDLs in microglial cells.Elena Grao-CrucesMaria C. Millan-LinaresMaria E. Martin-RubioRocio ToscanoSergio Barrientos-TrigoBeatriz BermudezSergio Montserrat-de la PazMDPI AGarticleobesityparaoxonaselipoproteinsoxidationmicroglianeuroinflammationBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1722, p 1722 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	obesity paraoxonase lipoproteins oxidation microglia neuroinflammation Biology (General) QH301-705.5
spellingShingle	obesity paraoxonase lipoproteins oxidation microglia neuroinflammation Biology (General) QH301-705.5 Elena Grao-Cruces Maria C. Millan-Linares Maria E. Martin-Rubio Rocio Toscano Sergio Barrientos-Trigo Beatriz Bermudez Sergio Montserrat-de la Paz Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells
description	High-density lipoproteins (HDLs) play an important role in reverse cholesterol transport and present antioxidant properties, among others. In the central nervous system (CNS), there are HDLs, where these lipoproteins could influence brain health. Owing to the new evidence of HDL functionality remodeling in obese patients, and the fact that obesity-associated metabolic disturbances is pro-inflammatory and pro-oxidant, the aim of this study was to investigate if HDL functions are depleted in obese patients and obesity-associated microenvironment. HDLs were isolated from normal-weight healthy (nwHDL) and obese men (obHDL). The oxHDL level was measured by malondialdehyde and 4-hydroxynoneal peroxided products. BV2 microglial cells were exposed to different concentrations of nwHDL and obHDL in different obesity-associated pro-inflammatory microenvironments. Our results showed that hyperleptinemia increased oxHDL levels. In addition, nwHDLs reduced pro-inflammatory cytokines’ release and M1 marker gene expression in BV2 microglial cells. Nevertheless, both nwHDL co-administered with LPS+leptin and obHDL promoted BV2 microglial activation and a higher pro-inflammatory cytokine production, thus confirming that obesity-associated metabolic disturbances reverse the antioxidant and anti-inflammatory properties of HDLs in microglial cells.
format	article
author	Elena Grao-Cruces Maria C. Millan-Linares Maria E. Martin-Rubio Rocio Toscano Sergio Barrientos-Trigo Beatriz Bermudez Sergio Montserrat-de la Paz
author_facet	Elena Grao-Cruces Maria C. Millan-Linares Maria E. Martin-Rubio Rocio Toscano Sergio Barrientos-Trigo Beatriz Bermudez Sergio Montserrat-de la Paz
author_sort	Elena Grao-Cruces
title	Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells
title_short	Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells
title_full	Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells
title_fullStr	Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells
title_full_unstemmed	Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells
title_sort	obesity-associated metabolic disturbances reverse the antioxidant and anti-inflammatory properties of high-density lipoproteins in microglial cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/19ae9c854b2c4aee9db4f89175a2945e
work_keys_str_mv	AT elenagraocruces obesityassociatedmetabolicdisturbancesreversetheantioxidantandantiinflammatorypropertiesofhighdensitylipoproteinsinmicroglialcells AT mariacmillanlinares obesityassociatedmetabolicdisturbancesreversetheantioxidantandantiinflammatorypropertiesofhighdensitylipoproteinsinmicroglialcells AT mariaemartinrubio obesityassociatedmetabolicdisturbancesreversetheantioxidantandantiinflammatorypropertiesofhighdensitylipoproteinsinmicroglialcells AT rociotoscano obesityassociatedmetabolicdisturbancesreversetheantioxidantandantiinflammatorypropertiesofhighdensitylipoproteinsinmicroglialcells AT sergiobarrientostrigo obesityassociatedmetabolicdisturbancesreversetheantioxidantandantiinflammatorypropertiesofhighdensitylipoproteinsinmicroglialcells AT beatrizbermudez obesityassociatedmetabolicdisturbancesreversetheantioxidantandantiinflammatorypropertiesofhighdensitylipoproteinsinmicroglialcells AT sergiomontserratdelapaz obesityassociatedmetabolicdisturbancesreversetheantioxidantandantiinflammatorypropertiesofhighdensitylipoproteinsinmicroglialcells
_version_	1718412879792701440

Obesity-Associated Metabolic Disturbances Reverse the Antioxidant and Anti-Inflammatory Properties of High-Density Lipoproteins in Microglial Cells

Ejemplares similares