Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing

Abstract Cells’ ability to sense mechanical cues in their environment is crucial for fundamental cellular processes, leading defects in mechanosensing to be linked to many diseases. The actin cross-linking protein Filamin has an important role in the conversion of mechanical forces into biochemical...

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Autores principales:	Jonne Seppälä, Rafael C. Bernardi, Tatu J. K. Haataja, Maarit Hellman, Olli T. Pentikäinen, Klaus Schulten, Perttu Permi, Jari Ylänne, Ulla Pentikäinen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19baf53cadc947c297dabc4e0149c6e8
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spelling	oai:doaj.org-article:19baf53cadc947c297dabc4e0149c6e82021-12-02T12:32:01ZSkeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing10.1038/s41598-017-04441-x2045-2322https://doaj.org/article/19baf53cadc947c297dabc4e0149c6e82017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04441-xhttps://doaj.org/toc/2045-2322Abstract Cells’ ability to sense mechanical cues in their environment is crucial for fundamental cellular processes, leading defects in mechanosensing to be linked to many diseases. The actin cross-linking protein Filamin has an important role in the conversion of mechanical forces into biochemical signals. Here, we reveal how mutations in Filamin genes known to cause Larsen syndrome and Frontometaphyseal dysplasia can affect the structure and therefore function of Filamin domains 16 and 17. Employing X-ray crystallography, the structure of these domains was first solved for the human Filamin B. The interaction seen between domains 16 and 17 is broken by shear force as revealed by steered molecular dynamics simulations. The effects of skeletal dysplasia associated mutations of the structure and mechanosensing properties of Filamin were studied by combining various experimental and theoretical techniques. The results showed that Larsen syndrome associated mutations destabilize or even unfold domain 17. Interestingly, those Filamin functions that are mediated via domain 17 interactions with other proteins are not necessarily affected as strongly interacting peptide binding to mutated domain 17 induces at least partial domain folding. Mutation associated to Frontometaphyseal dysplasia, in turn, transforms 16–17 fragment from compact to an elongated form destroying the force-regulated domain pair.Jonne SeppäläRafael C. BernardiTatu J. K. HaatajaMaarit HellmanOlli T. PentikäinenKlaus SchultenPerttu PermiJari YlänneUlla PentikäinenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonne Seppälä Rafael C. Bernardi Tatu J. K. Haataja Maarit Hellman Olli T. Pentikäinen Klaus Schulten Perttu Permi Jari Ylänne Ulla Pentikäinen Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing
description	Abstract Cells’ ability to sense mechanical cues in their environment is crucial for fundamental cellular processes, leading defects in mechanosensing to be linked to many diseases. The actin cross-linking protein Filamin has an important role in the conversion of mechanical forces into biochemical signals. Here, we reveal how mutations in Filamin genes known to cause Larsen syndrome and Frontometaphyseal dysplasia can affect the structure and therefore function of Filamin domains 16 and 17. Employing X-ray crystallography, the structure of these domains was first solved for the human Filamin B. The interaction seen between domains 16 and 17 is broken by shear force as revealed by steered molecular dynamics simulations. The effects of skeletal dysplasia associated mutations of the structure and mechanosensing properties of Filamin were studied by combining various experimental and theoretical techniques. The results showed that Larsen syndrome associated mutations destabilize or even unfold domain 17. Interestingly, those Filamin functions that are mediated via domain 17 interactions with other proteins are not necessarily affected as strongly interacting peptide binding to mutated domain 17 induces at least partial domain folding. Mutation associated to Frontometaphyseal dysplasia, in turn, transforms 16–17 fragment from compact to an elongated form destroying the force-regulated domain pair.
format	article
author	Jonne Seppälä Rafael C. Bernardi Tatu J. K. Haataja Maarit Hellman Olli T. Pentikäinen Klaus Schulten Perttu Permi Jari Ylänne Ulla Pentikäinen
author_facet	Jonne Seppälä Rafael C. Bernardi Tatu J. K. Haataja Maarit Hellman Olli T. Pentikäinen Klaus Schulten Perttu Permi Jari Ylänne Ulla Pentikäinen
author_sort	Jonne Seppälä
title	Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing
title_short	Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing
title_full	Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing
title_fullStr	Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing
title_full_unstemmed	Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing
title_sort	skeletal dysplasia mutations effect on human filamins’ structure and mechanosensing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/19baf53cadc947c297dabc4e0149c6e8
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Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing

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