Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.

Corky split vein caused by boron (B) deficiency in 'Newhall' Navel Orange was studied in the present research. The boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. Morphologic and anatomical surveys at four representative phases of corky split veins showed t...

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Autores principales:	Cheng-Quan Yang, Yong-Zhong Liu, Ji-Cui An, Shuang Li, Long-Fei Jin, Gao-Feng Zhou, Qing-Jiang Wei, Hui-Qing Yan, Nan-Nan Wang, Li-Na Fu, Xiao Liu, Xiao-Mei Hu, Ting-Shuai Yan, Shu-Ang Peng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19bba77164204c439e4de7834c6a3add
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spelling	oai:doaj.org-article:19bba77164204c439e4de7834c6a3add2021-11-18T07:42:54ZDigital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.1932-620310.1371/journal.pone.0065737https://doaj.org/article/19bba77164204c439e4de7834c6a3add2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23755275/?tool=EBIhttps://doaj.org/toc/1932-6203Corky split vein caused by boron (B) deficiency in 'Newhall' Navel Orange was studied in the present research. The boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. Morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. Digital gene expression (DGE) analysis was performed based on the Illumina HiSeq™ 2000 platform, which was applied to analyze the gene expression profilings of corky split veins at four morphologic phases. Over 5.3 million clean reads per library were successfully mapped to the reference database and more than 22897 mapped genes per library were simultaneously obtained. Analysis of the differentially expressed genes (DEGs) revealed that the expressions of genes associated with cytokinin signal transduction, cell division, vascular development, lignin biosynthesis and photosynthesis in corky split veins were all affected. The expressions of WOL and ARR12 involved in the cytokinin signal transduction pathway were up-regulated at 1(st) phase of corky split vein development. Furthermore, the expressions of some cell cycle genes, CYCs and CDKB, and vascular development genes, WOX4 and VND7, were up-regulated at the following 2(nd) and 3(rd) phases. These findings indicated that the cytokinin signal transduction pathway may play a role in initiating symptom observed in our study.Cheng-Quan YangYong-Zhong LiuJi-Cui AnShuang LiLong-Fei JinGao-Feng ZhouQing-Jiang WeiHui-Qing YanNan-Nan WangLi-Na FuXiao LiuXiao-Mei HuTing-Shuai YanShu-Ang PengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65737 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cheng-Quan Yang Yong-Zhong Liu Ji-Cui An Shuang Li Long-Fei Jin Gao-Feng Zhou Qing-Jiang Wei Hui-Qing Yan Nan-Nan Wang Li-Na Fu Xiao Liu Xiao-Mei Hu Ting-Shuai Yan Shu-Ang Peng Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.
description	Corky split vein caused by boron (B) deficiency in 'Newhall' Navel Orange was studied in the present research. The boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. Morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. Digital gene expression (DGE) analysis was performed based on the Illumina HiSeq™ 2000 platform, which was applied to analyze the gene expression profilings of corky split veins at four morphologic phases. Over 5.3 million clean reads per library were successfully mapped to the reference database and more than 22897 mapped genes per library were simultaneously obtained. Analysis of the differentially expressed genes (DEGs) revealed that the expressions of genes associated with cytokinin signal transduction, cell division, vascular development, lignin biosynthesis and photosynthesis in corky split veins were all affected. The expressions of WOL and ARR12 involved in the cytokinin signal transduction pathway were up-regulated at 1(st) phase of corky split vein development. Furthermore, the expressions of some cell cycle genes, CYCs and CDKB, and vascular development genes, WOX4 and VND7, were up-regulated at the following 2(nd) and 3(rd) phases. These findings indicated that the cytokinin signal transduction pathway may play a role in initiating symptom observed in our study.
format	article
author	Cheng-Quan Yang Yong-Zhong Liu Ji-Cui An Shuang Li Long-Fei Jin Gao-Feng Zhou Qing-Jiang Wei Hui-Qing Yan Nan-Nan Wang Li-Na Fu Xiao Liu Xiao-Mei Hu Ting-Shuai Yan Shu-Ang Peng
author_facet	Cheng-Quan Yang Yong-Zhong Liu Ji-Cui An Shuang Li Long-Fei Jin Gao-Feng Zhou Qing-Jiang Wei Hui-Qing Yan Nan-Nan Wang Li-Na Fu Xiao Liu Xiao-Mei Hu Ting-Shuai Yan Shu-Ang Peng
author_sort	Cheng-Quan Yang
title	Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.
title_short	Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.
title_full	Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.
title_fullStr	Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.
title_full_unstemmed	Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.
title_sort	digital gene expression analysis of corky split vein caused by boron deficiency in 'newhall' navel orange (citrus sinensis osbeck) for selecting differentially expressed genes related to vascular hypertrophy.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/19bba77164204c439e4de7834c6a3add
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Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.

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