EFFECT OF ERYTHROPOIETIN ON CYTOKINE PRODUCTION BY STEM CELLS
Erythropoietin (EPO) is mainly used to stimulate erythropoiesis. Its cytoprotective effects upon other cells of the human body and animals were recently shown, in particular, anti-apoptotic effect was observed. EPO effect upon the cells is mediated by interaction with erythropoietin receptor, with a...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | RU |
| Publicado: |
SPb RAACI
2019
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/19bc9f2ff9ff4520828495d0baef087d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:19bc9f2ff9ff4520828495d0baef087d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:19bc9f2ff9ff4520828495d0baef087d2021-11-18T08:03:49ZEFFECT OF ERYTHROPOIETIN ON CYTOKINE PRODUCTION BY STEM CELLS1563-06252313-741X10.15789/1563-0625-2019-5-861-868https://doaj.org/article/19bc9f2ff9ff4520828495d0baef087d2019-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1723https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XErythropoietin (EPO) is mainly used to stimulate erythropoiesis. Its cytoprotective effects upon other cells of the human body and animals were recently shown, in particular, anti-apoptotic effect was observed. EPO effect upon the cells is mediated by interaction with erythropoietin receptor, with a complex forming a heterodimeric bond with β-common chain (CD131). In the present work, we studied the changes in erythropoietin receptor expression, and production spectrum of biologically active molecules in bone marrow mononuclear cells (BM-MNC) of patients with coronary heart disease. The flow cytometric assays showed that short-term incubation of BM-MNC with erythropoietin caused increased expression of the erythropoietin receptors on hematopoietic stem cells and tended to reduce the number of endothelial progenitor cells carrying the erythropoietin receptors. Solid-phase enzyme immunoassay in conditioned media from BM-MNC revealed that long-term (72 hours) exposure of BM-MNC to erythropoietin promoted increased production of IL-1β, PDGF-AB, and Epo, if compared to the basal production level (p < 0.05). Short-term incubation of BM-MNC with erythropoietin (60 minutes) caused a significant increase in the IL-1β, PDGF-AB and CXCL-12 / SDF-1α production levels, as well as significant reduction in the IL-10 production levels compared to the basal levels (p < 0.05).A. P. LykovM. A. SurovtsevaO. V. PoveshchenkoN. A. BondarenkoI. I. KimA. M. ChernyvskiA. V. FomichevSPb RAACIarticlebone marrow stem cellserythropoietincytokineischemic heart diseaseImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 5, Pp 861-868 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
RU |
| topic |
bone marrow stem cells erythropoietin cytokine ischemic heart disease Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
bone marrow stem cells erythropoietin cytokine ischemic heart disease Immunologic diseases. Allergy RC581-607 A. P. Lykov M. A. Surovtseva O. V. Poveshchenko N. A. Bondarenko I. I. Kim A. M. Chernyvski A. V. Fomichev EFFECT OF ERYTHROPOIETIN ON CYTOKINE PRODUCTION BY STEM CELLS |
| description |
Erythropoietin (EPO) is mainly used to stimulate erythropoiesis. Its cytoprotective effects upon other cells of the human body and animals were recently shown, in particular, anti-apoptotic effect was observed. EPO effect upon the cells is mediated by interaction with erythropoietin receptor, with a complex forming a heterodimeric bond with β-common chain (CD131). In the present work, we studied the changes in erythropoietin receptor expression, and production spectrum of biologically active molecules in bone marrow mononuclear cells (BM-MNC) of patients with coronary heart disease. The flow cytometric assays showed that short-term incubation of BM-MNC with erythropoietin caused increased expression of the erythropoietin receptors on hematopoietic stem cells and tended to reduce the number of endothelial progenitor cells carrying the erythropoietin receptors. Solid-phase enzyme immunoassay in conditioned media from BM-MNC revealed that long-term (72 hours) exposure of BM-MNC to erythropoietin promoted increased production of IL-1β, PDGF-AB, and Epo, if compared to the basal production level (p < 0.05). Short-term incubation of BM-MNC with erythropoietin (60 minutes) caused a significant increase in the IL-1β, PDGF-AB and CXCL-12 / SDF-1α production levels, as well as significant reduction in the IL-10 production levels compared to the basal levels (p < 0.05). |
| format |
article |
| author |
A. P. Lykov M. A. Surovtseva O. V. Poveshchenko N. A. Bondarenko I. I. Kim A. M. Chernyvski A. V. Fomichev |
| author_facet |
A. P. Lykov M. A. Surovtseva O. V. Poveshchenko N. A. Bondarenko I. I. Kim A. M. Chernyvski A. V. Fomichev |
| author_sort |
A. P. Lykov |
| title |
EFFECT OF ERYTHROPOIETIN ON CYTOKINE PRODUCTION BY STEM CELLS |
| title_short |
EFFECT OF ERYTHROPOIETIN ON CYTOKINE PRODUCTION BY STEM CELLS |
| title_full |
EFFECT OF ERYTHROPOIETIN ON CYTOKINE PRODUCTION BY STEM CELLS |
| title_fullStr |
EFFECT OF ERYTHROPOIETIN ON CYTOKINE PRODUCTION BY STEM CELLS |
| title_full_unstemmed |
EFFECT OF ERYTHROPOIETIN ON CYTOKINE PRODUCTION BY STEM CELLS |
| title_sort |
effect of erythropoietin on cytokine production by stem cells |
| publisher |
SPb RAACI |
| publishDate |
2019 |
| url |
https://doaj.org/article/19bc9f2ff9ff4520828495d0baef087d |
| work_keys_str_mv |
AT aplykov effectoferythropoietinoncytokineproductionbystemcells AT masurovtseva effectoferythropoietinoncytokineproductionbystemcells AT ovpoveshchenko effectoferythropoietinoncytokineproductionbystemcells AT nabondarenko effectoferythropoietinoncytokineproductionbystemcells AT iikim effectoferythropoietinoncytokineproductionbystemcells AT amchernyvski effectoferythropoietinoncytokineproductionbystemcells AT avfomichev effectoferythropoietinoncytokineproductionbystemcells |
| _version_ |
1718422335777669120 |