Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis

Intestinal goblet cells maintain the protective epithelial barrier through mucus secretion and yet sample lumenal substances for immune processing through formation of goblet cell associated antigen passages (GAPs). The cellular biology of GAPs and how these divergent processes are balanced and regu...

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Autores principales:	Jenny K Gustafsson, Jazmyne E Davis, Tracy Rappai, Keely G McDonald, Devesha H Kulkarni, Kathryn A Knoop, Simon P Hogan, James AJ Fitzpatrick, Wayne I Lencer, Rodney D Newberry
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	intestine goblet cells antigen sampling Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/19bdc7ef1aaa471e92f3cef5b5aedbdf
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spelling	oai:doaj.org-article:19bdc7ef1aaa471e92f3cef5b5aedbdf2021-11-16T17:29:43ZIntestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis10.7554/eLife.672922050-084Xe67292https://doaj.org/article/19bdc7ef1aaa471e92f3cef5b5aedbdf2021-10-01T00:00:00Zhttps://elifesciences.org/articles/67292https://doaj.org/toc/2050-084XIntestinal goblet cells maintain the protective epithelial barrier through mucus secretion and yet sample lumenal substances for immune processing through formation of goblet cell associated antigen passages (GAPs). The cellular biology of GAPs and how these divergent processes are balanced and regulated by goblet cells remains unknown. Using high-resolution light and electron microscopy, we found that in mice, GAPs were formed by an acetylcholine (ACh)-dependent endocytic event remarkable for delivery of fluid-phase cargo retrograde into the trans-golgi network and across the cell by transcytosis – in addition to the expected transport of fluid-phase cargo by endosomes to multi-vesicular bodies and lysosomes. While ACh also induced goblet cells to secrete mucins, ACh-induced GAP formation and mucin secretion were functionally independent and mediated by different receptors and signaling pathways, enabling goblet cells to differentially regulate these processes to accommodate the dynamically changing demands of the mucosal environment for barrier maintenance and sampling of lumenal substances.Jenny K GustafssonJazmyne E DavisTracy RappaiKeely G McDonaldDevesha H KulkarniKathryn A KnoopSimon P HoganJames AJ FitzpatrickWayne I LencerRodney D NewberryeLife Sciences Publications Ltdarticleintestinegoblet cellsantigen samplingMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	intestine goblet cells antigen sampling Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	intestine goblet cells antigen sampling Medicine R Science Q Biology (General) QH301-705.5 Jenny K Gustafsson Jazmyne E Davis Tracy Rappai Keely G McDonald Devesha H Kulkarni Kathryn A Knoop Simon P Hogan James AJ Fitzpatrick Wayne I Lencer Rodney D Newberry Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis
description	Intestinal goblet cells maintain the protective epithelial barrier through mucus secretion and yet sample lumenal substances for immune processing through formation of goblet cell associated antigen passages (GAPs). The cellular biology of GAPs and how these divergent processes are balanced and regulated by goblet cells remains unknown. Using high-resolution light and electron microscopy, we found that in mice, GAPs were formed by an acetylcholine (ACh)-dependent endocytic event remarkable for delivery of fluid-phase cargo retrograde into the trans-golgi network and across the cell by transcytosis – in addition to the expected transport of fluid-phase cargo by endosomes to multi-vesicular bodies and lysosomes. While ACh also induced goblet cells to secrete mucins, ACh-induced GAP formation and mucin secretion were functionally independent and mediated by different receptors and signaling pathways, enabling goblet cells to differentially regulate these processes to accommodate the dynamically changing demands of the mucosal environment for barrier maintenance and sampling of lumenal substances.
format	article
author	Jenny K Gustafsson Jazmyne E Davis Tracy Rappai Keely G McDonald Devesha H Kulkarni Kathryn A Knoop Simon P Hogan James AJ Fitzpatrick Wayne I Lencer Rodney D Newberry
author_facet	Jenny K Gustafsson Jazmyne E Davis Tracy Rappai Keely G McDonald Devesha H Kulkarni Kathryn A Knoop Simon P Hogan James AJ Fitzpatrick Wayne I Lencer Rodney D Newberry
author_sort	Jenny K Gustafsson
title	Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis
title_short	Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis
title_full	Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis
title_fullStr	Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis
title_full_unstemmed	Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis
title_sort	intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/19bdc7ef1aaa471e92f3cef5b5aedbdf
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Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis

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