Colour-assisted variation in elytral ICP-OES-based ionomics in an aposematic beetle

Abstract Very little is known about how the elemental composition (ionome) of an insect cuticle varies as a result of different colouration. Using inductively-coupled plasma optical emission spectrometry (ICP-OES), we established ionomic profiles in microsamples of two adjacent regions of an insect...

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Autores principales:	Grzegorz Orłowski, Przemysław Niedzielski, Jerzy Karg, Jędrzej Proch
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19bfef8447a443eaa8412acb1583571a
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spelling	oai:doaj.org-article:19bfef8447a443eaa8412acb1583571a2021-12-02T13:33:59ZColour-assisted variation in elytral ICP-OES-based ionomics in an aposematic beetle10.1038/s41598-020-79329-42045-2322https://doaj.org/article/19bfef8447a443eaa8412acb1583571a2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79329-4https://doaj.org/toc/2045-2322Abstract Very little is known about how the elemental composition (ionome) of an insect cuticle varies as a result of different colouration. Using inductively-coupled plasma optical emission spectrometry (ICP-OES), we established ionomic profiles in microsamples of two adjacent regions of an insect cuticle with a contrasting colour pattern, namely, the black and orange regions of the elytra of the aposematic burying beetle Nicrophorus vespillo. The analysis revealed 53 elements (ranging in atomic weight from Na to Bi) occurring above the detection limit. The frequency of detectability of individual elements varied strongly, and only ten elements (Ba, Cu, Fe, K, Mg, Mn, P, Rb, Sb and Zn) were present in concentrations exceeding the detection limit in all the samples. The sum of concentrations of all elements in the orange regions of the elytra was 9% lower than in the black ones. The opposite distribution was displayed by the rare earth elements (REEs), the sum of which was 17% lower in the black elytral regions than in the orange ones. The concentrations of six elements were significantly higher in the black than in the orange regions: Al (by 97%), Cu (41%), Mn (14%), Na (46%), Se (97%) and W (47%). The concentrations of essential elements measured in both the black and orange regions exhibited very considerable variance: Ca (σ2 = 1834; 1882, respectively), K (145; 82) P (97; 76), Na (84; 53), Mg (24; 26) and Ba (9; 13). This, in part, could be attributed to individual differences, e.g. those resulting from the consumption of animal carcasses of different quality/chemical composition, but interference between elements and the consequent lowering of measurement quality are also possible. We highlight the fact that deeper insight into the basic relationship between insect colouration and variation in elemental composition requires micro-sampling of the homogeneous layers of an exoskeleton.Grzegorz OrłowskiPrzemysław NiedzielskiJerzy KargJędrzej ProchNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Grzegorz Orłowski Przemysław Niedzielski Jerzy Karg Jędrzej Proch Colour-assisted variation in elytral ICP-OES-based ionomics in an aposematic beetle
description	Abstract Very little is known about how the elemental composition (ionome) of an insect cuticle varies as a result of different colouration. Using inductively-coupled plasma optical emission spectrometry (ICP-OES), we established ionomic profiles in microsamples of two adjacent regions of an insect cuticle with a contrasting colour pattern, namely, the black and orange regions of the elytra of the aposematic burying beetle Nicrophorus vespillo. The analysis revealed 53 elements (ranging in atomic weight from Na to Bi) occurring above the detection limit. The frequency of detectability of individual elements varied strongly, and only ten elements (Ba, Cu, Fe, K, Mg, Mn, P, Rb, Sb and Zn) were present in concentrations exceeding the detection limit in all the samples. The sum of concentrations of all elements in the orange regions of the elytra was 9% lower than in the black ones. The opposite distribution was displayed by the rare earth elements (REEs), the sum of which was 17% lower in the black elytral regions than in the orange ones. The concentrations of six elements were significantly higher in the black than in the orange regions: Al (by 97%), Cu (41%), Mn (14%), Na (46%), Se (97%) and W (47%). The concentrations of essential elements measured in both the black and orange regions exhibited very considerable variance: Ca (σ2 = 1834; 1882, respectively), K (145; 82) P (97; 76), Na (84; 53), Mg (24; 26) and Ba (9; 13). This, in part, could be attributed to individual differences, e.g. those resulting from the consumption of animal carcasses of different quality/chemical composition, but interference between elements and the consequent lowering of measurement quality are also possible. We highlight the fact that deeper insight into the basic relationship between insect colouration and variation in elemental composition requires micro-sampling of the homogeneous layers of an exoskeleton.
format	article
author	Grzegorz Orłowski Przemysław Niedzielski Jerzy Karg Jędrzej Proch
author_facet	Grzegorz Orłowski Przemysław Niedzielski Jerzy Karg Jędrzej Proch
author_sort	Grzegorz Orłowski
title	Colour-assisted variation in elytral ICP-OES-based ionomics in an aposematic beetle
title_short	Colour-assisted variation in elytral ICP-OES-based ionomics in an aposematic beetle
title_full	Colour-assisted variation in elytral ICP-OES-based ionomics in an aposematic beetle
title_fullStr	Colour-assisted variation in elytral ICP-OES-based ionomics in an aposematic beetle
title_full_unstemmed	Colour-assisted variation in elytral ICP-OES-based ionomics in an aposematic beetle
title_sort	colour-assisted variation in elytral icp-oes-based ionomics in an aposematic beetle
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/19bfef8447a443eaa8412acb1583571a
work_keys_str_mv	AT grzegorzorłowski colourassistedvariationinelytralicpoesbasedionomicsinanaposematicbeetle AT przemysławniedzielski colourassistedvariationinelytralicpoesbasedionomicsinanaposematicbeetle AT jerzykarg colourassistedvariationinelytralicpoesbasedionomicsinanaposematicbeetle AT jedrzejproch colourassistedvariationinelytralicpoesbasedionomicsinanaposematicbeetle
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Colour-assisted variation in elytral ICP-OES-based ionomics in an aposematic beetle

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