Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe

Jun Zhou*, Yi-Ting Tsai*, Hong Weng, Ewin N Tang, Ashwin Nair, Digant P Dav&eacute;, Liping Tang, Department of Bioengineering, University of Texas at Arlington, Arlington, TX *Both authors contributed equally to this workAbstract: Neutrophils play an important role in implant-mediated infla...

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Autores principales:	Zhou J, Nair A, Dav&eacute; DP, Tang L, Tang EN, Weng H, Tsai Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/19ca7ede94ad4e508193b86760f5f5f6
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spelling	oai:doaj.org-article:19ca7ede94ad4e508193b86760f5f5f62021-12-02T02:44:04ZReal-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe1176-91141178-2013https://doaj.org/article/19ca7ede94ad4e508193b86760f5f5f62012-05-01T00:00:00Zhttp://www.dovepress.com/real-time-detection-of-implant-associated-neutrophil-responses-using-a-a9814https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jun Zhou, Yi-Ting Tsai, Hong Weng, Ewin N Tang, Ashwin Nair, Digant P Dav&eacute;, Liping Tang, Department of Bioengineering, University of Texas at Arlington, Arlington, TX *Both authors contributed equally to this workAbstract: Neutrophils play an important role in implant-mediated inflammation and infection. Unfortunately, current methods which monitor neutrophil activity, including enzyme measurements and histological evaluation, require many animals and cannot be used to accurately depict the dynamic cellular responses. To understand the neutrophil interactions around implant-mediated inflammation and infection it is critical to develop methods which can monitor in vivo cellular activity in real time. In this study, formyl peptide receptor (FPR)-targeting near-infrared nanoprobes were fabricated. This was accomplished by conjugating near-infrared dye with specific peptides having a high affinity to the FPRs present on activated neutrophils. The ability of FPR-targeting nanoprobes to detect and quantify activated neutrophils was assessed both in vitro and in vivo. As expected, FPR-targeting nanoprobes preferentially accumulated on activated neutrophils in vitro. Following transplantation, FPR-targeting nanoprobes preferentially accumulated at the biomaterial implantation site. Equally important, a strong relationship was observed between the extent of fluorescence intensity in vivo and the number of recruited neutrophils at the implantation site. Furthermore, FPR-targeting nanoprobes may be used to detect and quantify the number of neutrophils responding to a catheter-associated infection. The results show that FPR-targeting nanoprobes may serve as a powerful tool to monitor and measure the extent of neutrophil responses to biomaterial implants in vivo.Keywords: in vivo imaging, nanoprobe, neutrophils, inflammation, biocompatibilityZhou JNair ADav&eacute; DPTang LTang ENWeng HTsai YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2057-2068 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhou J Nair A Dav&eacute; DP Tang L Tang EN Weng H Tsai Y Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe
description	Jun Zhou, Yi-Ting Tsai, Hong Weng, Ewin N Tang, Ashwin Nair, Digant P Dav&eacute;, Liping Tang, Department of Bioengineering, University of Texas at Arlington, Arlington, TX *Both authors contributed equally to this workAbstract: Neutrophils play an important role in implant-mediated inflammation and infection. Unfortunately, current methods which monitor neutrophil activity, including enzyme measurements and histological evaluation, require many animals and cannot be used to accurately depict the dynamic cellular responses. To understand the neutrophil interactions around implant-mediated inflammation and infection it is critical to develop methods which can monitor in vivo cellular activity in real time. In this study, formyl peptide receptor (FPR)-targeting near-infrared nanoprobes were fabricated. This was accomplished by conjugating near-infrared dye with specific peptides having a high affinity to the FPRs present on activated neutrophils. The ability of FPR-targeting nanoprobes to detect and quantify activated neutrophils was assessed both in vitro and in vivo. As expected, FPR-targeting nanoprobes preferentially accumulated on activated neutrophils in vitro. Following transplantation, FPR-targeting nanoprobes preferentially accumulated at the biomaterial implantation site. Equally important, a strong relationship was observed between the extent of fluorescence intensity in vivo and the number of recruited neutrophils at the implantation site. Furthermore, FPR-targeting nanoprobes may be used to detect and quantify the number of neutrophils responding to a catheter-associated infection. The results show that FPR-targeting nanoprobes may serve as a powerful tool to monitor and measure the extent of neutrophil responses to biomaterial implants in vivo.Keywords: in vivo imaging, nanoprobe, neutrophils, inflammation, biocompatibility
format	article
author	Zhou J Nair A Dav&eacute; DP Tang L Tang EN Weng H Tsai Y
author_facet	Zhou J Nair A Dav&eacute; DP Tang L Tang EN Weng H Tsai Y
author_sort	Zhou J
title	Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe
title_short	Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe
title_full	Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe
title_fullStr	Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe
title_full_unstemmed	Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe
title_sort	real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting nir nanoprobe
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/19ca7ede94ad4e508193b86760f5f5f6
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Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe

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