Populus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis
Abstract During symbiosis, organisms use a range of metabolic and protein-based signals to communicate. Of these protein signals, one class is defined as ‘effectors’, i.e., small secreted proteins (SSPs) that cause phenotypical and physiological changes in another organism. To date, protein-based ef...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/19d3b94196694c16b3ffc44d6ff995b9 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:19d3b94196694c16b3ffc44d6ff995b9 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:19d3b94196694c16b3ffc44d6ff995b92021-12-02T11:41:10ZPopulus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis10.1038/s41598-017-00400-82045-2322https://doaj.org/article/19d3b94196694c16b3ffc44d6ff995b92017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00400-8https://doaj.org/toc/2045-2322Abstract During symbiosis, organisms use a range of metabolic and protein-based signals to communicate. Of these protein signals, one class is defined as ‘effectors’, i.e., small secreted proteins (SSPs) that cause phenotypical and physiological changes in another organism. To date, protein-based effectors have been described in aphids, nematodes, fungi and bacteria. Using RNA sequencing of Populus trichocarpa roots in mutualistic symbiosis with the ectomycorrhizal fungus Laccaria bicolor, we sought to determine if host plants also contain genes encoding effector-like proteins. We identified 417 plant-encoded putative SSPs that were significantly regulated during this interaction, including 161 SSPs specific to P. trichocarpa and 15 SSPs exhibiting expansion in Populus and closely related lineages. We demonstrate that a subset of these SSPs can enter L. bicolor hyphae, localize to the nucleus and affect hyphal growth and morphology. We conclude that plants encode proteins that appear to function as effector proteins that may regulate symbiotic associations.Jonathan M. PlettHengfu YinRitesh MewalalRongbin HuTing LiPriya RanjanSara JawdyHenrique C. De PaoliGeorge ButlerTessa Maureen Burch-SmithHao-Bo GuoChun Ju ChenAnnegret KohlerIan C. AndersonJessy L. LabbéFrancis MartinGerald A. TuskanXiaohan YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Jonathan M. Plett Hengfu Yin Ritesh Mewalal Rongbin Hu Ting Li Priya Ranjan Sara Jawdy Henrique C. De Paoli George Butler Tessa Maureen Burch-Smith Hao-Bo Guo Chun Ju Chen Annegret Kohler Ian C. Anderson Jessy L. Labbé Francis Martin Gerald A. Tuskan Xiaohan Yang Populus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis |
| description |
Abstract During symbiosis, organisms use a range of metabolic and protein-based signals to communicate. Of these protein signals, one class is defined as ‘effectors’, i.e., small secreted proteins (SSPs) that cause phenotypical and physiological changes in another organism. To date, protein-based effectors have been described in aphids, nematodes, fungi and bacteria. Using RNA sequencing of Populus trichocarpa roots in mutualistic symbiosis with the ectomycorrhizal fungus Laccaria bicolor, we sought to determine if host plants also contain genes encoding effector-like proteins. We identified 417 plant-encoded putative SSPs that were significantly regulated during this interaction, including 161 SSPs specific to P. trichocarpa and 15 SSPs exhibiting expansion in Populus and closely related lineages. We demonstrate that a subset of these SSPs can enter L. bicolor hyphae, localize to the nucleus and affect hyphal growth and morphology. We conclude that plants encode proteins that appear to function as effector proteins that may regulate symbiotic associations. |
| format |
article |
| author |
Jonathan M. Plett Hengfu Yin Ritesh Mewalal Rongbin Hu Ting Li Priya Ranjan Sara Jawdy Henrique C. De Paoli George Butler Tessa Maureen Burch-Smith Hao-Bo Guo Chun Ju Chen Annegret Kohler Ian C. Anderson Jessy L. Labbé Francis Martin Gerald A. Tuskan Xiaohan Yang |
| author_facet |
Jonathan M. Plett Hengfu Yin Ritesh Mewalal Rongbin Hu Ting Li Priya Ranjan Sara Jawdy Henrique C. De Paoli George Butler Tessa Maureen Burch-Smith Hao-Bo Guo Chun Ju Chen Annegret Kohler Ian C. Anderson Jessy L. Labbé Francis Martin Gerald A. Tuskan Xiaohan Yang |
| author_sort |
Jonathan M. Plett |
| title |
Populus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis |
| title_short |
Populus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis |
| title_full |
Populus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis |
| title_fullStr |
Populus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis |
| title_full_unstemmed |
Populus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis |
| title_sort |
populus trichocarpa encodes small, effector-like secreted proteins that are highly induced during mutualistic symbiosis |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/19d3b94196694c16b3ffc44d6ff995b9 |
| work_keys_str_mv |
AT jonathanmplett populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT hengfuyin populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT riteshmewalal populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT rongbinhu populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT tingli populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT priyaranjan populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT sarajawdy populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT henriquecdepaoli populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT georgebutler populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT tessamaureenburchsmith populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT haoboguo populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT chunjuchen populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT annegretkohler populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT iancanderson populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT jessyllabbe populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT francismartin populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT geraldatuskan populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis AT xiaohanyang populustrichocarpaencodessmalleffectorlikesecretedproteinsthatarehighlyinducedduringmutualisticsymbiosis |
| _version_ |
1718395447387619328 |