An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb

<p>In order to develop a protocol for the effective micropropagation of the important medicinal plant Withania coagulans (Stocks) Dunal, the effects of different concentrations and combinations of growth regulators on the nodal explants in two independent experiments were investigated. For sho...

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Autores principales:	Pari DEHVARI-NAGAN, Hosein ABBASPOUR, Mohammad Hasan ASARE, Sara SAADATMAND
Formato:	article
Lenguaje:	EN SL
Publicado:	University of Ljubljana, Biotechnical Faculty 2021
Materias:	withania coagulans micropropagation in vitro nodal explant phloroglucinol Agriculture S
Acceso en línea:	https://doaj.org/article/19d933981aa541ecb65563f803762e0b
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spelling	oai:doaj.org-article:19d933981aa541ecb65563f803762e0b2021-11-10T09:25:10ZAn efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb1581-91751854-194110.14720/aas.2021.117.2.1754https://doaj.org/article/19d933981aa541ecb65563f803762e0b2021-07-01T00:00:00Zhttp://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/1754https://doaj.org/toc/1581-9175https://doaj.org/toc/1854-1941<p>In order to develop a protocol for the effective micropropagation of the important medicinal plant Withania coagulans (Stocks) Dunal, the effects of different concentrations and combinations of growth regulators on the nodal explants in two independent experiments were investigated. For shooting, a MS medium fortified with different concentrations and combinations of IBA (0.01, 0.1 and 0.5 mg l-1), BA (0.5, 1 and 2 mg l-1), Kin (0.5 and 1 mg l-1), PG (0.5 mg l-1) and GA (0.5 mg l-1) was used and the highest shooting response, shoot number and shoot length were obtained in the MS + IBA (0.01 mg l-1) + BA (0.5 mg l-1) + PG (0.5 mg l-1) + GA (0.5 mg l-1) treatment. In the second experiment, the effect of MS supplemented with different combinations and concentrations of IBA (0.1, 0.5, 1 and 2 mg l-1), NAA (0.1 and 1 mg l-1) and PG (1 mg l-1) on rooting of the nodal explants was investigated, which showed that the highest rooting response (%) was observed in the MS fortified with NAA (0.1 mg l-1), NAA (1 mg l-1), NAA (0.1 mg l-1) + PG (1 mg l-1), and NAA (1 mg l-1) + PG (1 mg l-1) treatments, as well as the highest number of roots at NAA (0.1 mg l-1) and the highest root length at IBA (1 mg l-1). Our findings highlight a complete micropropagation method for W. coagulans from the nodal explant that can make a significant contribution to the development of W. coagulans material for medical applications.</p>Pari DEHVARI-NAGANHosein ABBASPOURMohammad Hasan ASARESara SAADATMANDUniversity of Ljubljana, Biotechnical Facultyarticlewithania coagulansmicropropagationin vitronodal explantphloroglucinolAgricultureSENSLActa Agriculturae Slovenica, Vol 117, Iss 2, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN SL
topic	withania coagulans micropropagation in vitro nodal explant phloroglucinol Agriculture S
spellingShingle	withania coagulans micropropagation in vitro nodal explant phloroglucinol Agriculture S Pari DEHVARI-NAGAN Hosein ABBASPOUR Mohammad Hasan ASARE Sara SAADATMAND An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb
description	<p>In order to develop a protocol for the effective micropropagation of the important medicinal plant Withania coagulans (Stocks) Dunal, the effects of different concentrations and combinations of growth regulators on the nodal explants in two independent experiments were investigated. For shooting, a MS medium fortified with different concentrations and combinations of IBA (0.01, 0.1 and 0.5 mg l-1), BA (0.5, 1 and 2 mg l-1), Kin (0.5 and 1 mg l-1), PG (0.5 mg l-1) and GA (0.5 mg l-1) was used and the highest shooting response, shoot number and shoot length were obtained in the MS + IBA (0.01 mg l-1) + BA (0.5 mg l-1) + PG (0.5 mg l-1) + GA (0.5 mg l-1) treatment. In the second experiment, the effect of MS supplemented with different combinations and concentrations of IBA (0.1, 0.5, 1 and 2 mg l-1), NAA (0.1 and 1 mg l-1) and PG (1 mg l-1) on rooting of the nodal explants was investigated, which showed that the highest rooting response (%) was observed in the MS fortified with NAA (0.1 mg l-1), NAA (1 mg l-1), NAA (0.1 mg l-1) + PG (1 mg l-1), and NAA (1 mg l-1) + PG (1 mg l-1) treatments, as well as the highest number of roots at NAA (0.1 mg l-1) and the highest root length at IBA (1 mg l-1). Our findings highlight a complete micropropagation method for W. coagulans from the nodal explant that can make a significant contribution to the development of W. coagulans material for medical applications.</p>
format	article
author	Pari DEHVARI-NAGAN Hosein ABBASPOUR Mohammad Hasan ASARE Sara SAADATMAND
author_facet	Pari DEHVARI-NAGAN Hosein ABBASPOUR Mohammad Hasan ASARE Sara SAADATMAND
author_sort	Pari DEHVARI-NAGAN
title	An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb
title_short	An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb
title_full	An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb
title_fullStr	An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb
title_full_unstemmed	An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb
title_sort	efficient protocol for in vitro regeneration from the nodal explants of withania coagulans (stocks) dunal: a valuable medicinal herb
publisher	University of Ljubljana, Biotechnical Faculty
publishDate	2021
url	https://doaj.org/article/19d933981aa541ecb65563f803762e0b
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An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb

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