Time course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan
Abstract The accurate and prompt diagnosis of SARS-CoV-2 infection is required for the control and treatment of the coronavirus infection disease 2019 (COVID-19). In this study, we aimed to investigate the time courses of the anti-severe acute corona respiratory syndrome coronavirus 2 (SARS-CoV-2) I...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/19da7f61f2594cf98ae23a55b9929544 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:19da7f61f2594cf98ae23a55b9929544 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:19da7f61f2594cf98ae23a55b99295442021-12-02T10:44:08ZTime course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan10.1038/s41598-021-82428-52045-2322https://doaj.org/article/19da7f61f2594cf98ae23a55b99295442021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82428-5https://doaj.org/toc/2045-2322Abstract The accurate and prompt diagnosis of SARS-CoV-2 infection is required for the control and treatment of the coronavirus infection disease 2019 (COVID-19). In this study, we aimed to investigate the time courses of the anti-severe acute corona respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM and IgG titers and to evaluate the sensitivity and specificity of such tests according to the specific day after the onset of COVID-19 among a patient population in Japan. We measured the titers of SARS-CoV-2 IgM and IgG in sera from 105 subjects, including 26 symptomatic COVID-19 patients, using chemiluminescent immunoassay (CLIA) methods utilizing magnetic beads coated with SARS-CoV-2 nucleocapsid protein and spike protein. The results of a ROC analysis suggested the possibility that the cutoff values in Japan might be lower than the manufacturer’s reported cutoff (10 AU/mL): 1 AU/mL for IgM and 5 AU/mL for IgG. The sensitivity of the test before Day 8 after symptom onset was less than 50%; at Days 9–10, however, we obtained a much higher sensitivity of 81.8% for both IgM and IgG. At 15 days or later after symptom onset, the SARS-CoV-2 IgG test had a sensitivity of 100%. These results suggest that if the number of days since disease onset is taken into consideration, these antibody tests could be very useful for the diagnosis of COVID-19 and similar diseases.Yuki NakanoMakoto KuranoYoshifumi MoritaTakuya ShimuraRin YokoyamaChungen QianFuzhen XiaFan HeYoshiro KishiJun OkadaNaoyuki YoshikawaYutaka NaguraHitoshi OkazakiKyoji MoriyaYasuyuki SetoTatsuhiko KodamaYutaka YatomiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Yuki Nakano Makoto Kurano Yoshifumi Morita Takuya Shimura Rin Yokoyama Chungen Qian Fuzhen Xia Fan He Yoshiro Kishi Jun Okada Naoyuki Yoshikawa Yutaka Nagura Hitoshi Okazaki Kyoji Moriya Yasuyuki Seto Tatsuhiko Kodama Yutaka Yatomi Time course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan |
| description |
Abstract The accurate and prompt diagnosis of SARS-CoV-2 infection is required for the control and treatment of the coronavirus infection disease 2019 (COVID-19). In this study, we aimed to investigate the time courses of the anti-severe acute corona respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM and IgG titers and to evaluate the sensitivity and specificity of such tests according to the specific day after the onset of COVID-19 among a patient population in Japan. We measured the titers of SARS-CoV-2 IgM and IgG in sera from 105 subjects, including 26 symptomatic COVID-19 patients, using chemiluminescent immunoassay (CLIA) methods utilizing magnetic beads coated with SARS-CoV-2 nucleocapsid protein and spike protein. The results of a ROC analysis suggested the possibility that the cutoff values in Japan might be lower than the manufacturer’s reported cutoff (10 AU/mL): 1 AU/mL for IgM and 5 AU/mL for IgG. The sensitivity of the test before Day 8 after symptom onset was less than 50%; at Days 9–10, however, we obtained a much higher sensitivity of 81.8% for both IgM and IgG. At 15 days or later after symptom onset, the SARS-CoV-2 IgG test had a sensitivity of 100%. These results suggest that if the number of days since disease onset is taken into consideration, these antibody tests could be very useful for the diagnosis of COVID-19 and similar diseases. |
| format |
article |
| author |
Yuki Nakano Makoto Kurano Yoshifumi Morita Takuya Shimura Rin Yokoyama Chungen Qian Fuzhen Xia Fan He Yoshiro Kishi Jun Okada Naoyuki Yoshikawa Yutaka Nagura Hitoshi Okazaki Kyoji Moriya Yasuyuki Seto Tatsuhiko Kodama Yutaka Yatomi |
| author_facet |
Yuki Nakano Makoto Kurano Yoshifumi Morita Takuya Shimura Rin Yokoyama Chungen Qian Fuzhen Xia Fan He Yoshiro Kishi Jun Okada Naoyuki Yoshikawa Yutaka Nagura Hitoshi Okazaki Kyoji Moriya Yasuyuki Seto Tatsuhiko Kodama Yutaka Yatomi |
| author_sort |
Yuki Nakano |
| title |
Time course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan |
| title_short |
Time course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan |
| title_full |
Time course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan |
| title_fullStr |
Time course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan |
| title_full_unstemmed |
Time course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan |
| title_sort |
time course of the sensitivity and specificity of anti-sars-cov-2 igm and igg antibodies for symptomatic covid-19 in japan |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/19da7f61f2594cf98ae23a55b9929544 |
| work_keys_str_mv |
AT yukinakano timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT makotokurano timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT yoshifumimorita timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT takuyashimura timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT rinyokoyama timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT chungenqian timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT fuzhenxia timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT fanhe timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT yoshirokishi timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT junokada timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT naoyukiyoshikawa timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT yutakanagura timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT hitoshiokazaki timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT kyojimoriya timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT yasuyukiseto timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT tatsuhikokodama timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan AT yutakayatomi timecourseofthesensitivityandspecificityofantisarscov2igmandiggantibodiesforsymptomaticcovid19injapan |
| _version_ |
1718396811927879680 |