The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease

Lactic acid (LA) is short-chain fatty acid, such as butyric acid and propionic acid, that is produced as a metabolite of lactic acid bacteria, including periodontopathic bacteria. These short-chain fatty acids have positive effects on human health but can also have negative effects, such as the prom...

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Autores principales:	Taichi Ishikawa, Daisuke Sasaki, Ryo Aizawa, Matsuo Yamamoto, Takashi Yaegashi, Tarou Irié, Minoru Sasaki
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell cycle junctional epithelium lactic acid periodontal disease short-chain fatty acids Medicine R
Acceso en línea:	https://doaj.org/article/19db7b3c362c487583d9b0d1515d0392
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spelling	oai:doaj.org-article:19db7b3c362c487583d9b0d1515d03922021-11-25T18:38:48ZThe Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease10.3390/pathogens101115072076-0817https://doaj.org/article/19db7b3c362c487583d9b0d1515d03922021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1507https://doaj.org/toc/2076-0817Lactic acid (LA) is short-chain fatty acid, such as butyric acid and propionic acid, that is produced as a metabolite of lactic acid bacteria, including periodontopathic bacteria. These short-chain fatty acids have positive effects on human health but can also have negative effects, such as the promotion of periodontal disease (PD), which is caused by periodontal pathogens present in the gingival sulcus. PD is characterized by apical migration of junctional epithelium, deepening of pockets, and alveolar bone loss. Thus, the junctional epithelial cells that form the bottom of the gingival sulcus are extremely important in investigating the pathophysiology of PD. The aim of this study was to investigate the effect of LA on wound healing, cell growth, cell cycle kinetics, and gene expression of cultured junctional epithelium cells. The results showed that stimulation with 10 mM LA slowed wound healing of the junctional epithelial cell layer and arrested the cell cycle in the G0/G1 (early cell cycle) phase, thereby inhibiting cell growth. However, cell destruction was not observed. LA also enhanced mRNA expression of integrin α5, interleukin (IL)-6, IL-8, intercellular adhesion molecule-1, and receptor activator of nuclear factor kappa-B ligand. The results of this study suggest that stimulation of junctional epithelial cells with high concentrations of LA could exacerbate PD, similarly to butyric acid and propionic acid.Taichi IshikawaDaisuke SasakiRyo AizawaMatsuo YamamotoTakashi YaegashiTarou IriéMinoru SasakiMDPI AGarticlecell cyclejunctional epitheliumlactic acidperiodontal diseaseshort-chain fatty acidsMedicineRENPathogens, Vol 10, Iss 1507, p 1507 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell cycle junctional epithelium lactic acid periodontal disease short-chain fatty acids Medicine R
spellingShingle	cell cycle junctional epithelium lactic acid periodontal disease short-chain fatty acids Medicine R Taichi Ishikawa Daisuke Sasaki Ryo Aizawa Matsuo Yamamoto Takashi Yaegashi Tarou Irié Minoru Sasaki The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease
description	Lactic acid (LA) is short-chain fatty acid, such as butyric acid and propionic acid, that is produced as a metabolite of lactic acid bacteria, including periodontopathic bacteria. These short-chain fatty acids have positive effects on human health but can also have negative effects, such as the promotion of periodontal disease (PD), which is caused by periodontal pathogens present in the gingival sulcus. PD is characterized by apical migration of junctional epithelium, deepening of pockets, and alveolar bone loss. Thus, the junctional epithelial cells that form the bottom of the gingival sulcus are extremely important in investigating the pathophysiology of PD. The aim of this study was to investigate the effect of LA on wound healing, cell growth, cell cycle kinetics, and gene expression of cultured junctional epithelium cells. The results showed that stimulation with 10 mM LA slowed wound healing of the junctional epithelial cell layer and arrested the cell cycle in the G0/G1 (early cell cycle) phase, thereby inhibiting cell growth. However, cell destruction was not observed. LA also enhanced mRNA expression of integrin α5, interleukin (IL)-6, IL-8, intercellular adhesion molecule-1, and receptor activator of nuclear factor kappa-B ligand. The results of this study suggest that stimulation of junctional epithelial cells with high concentrations of LA could exacerbate PD, similarly to butyric acid and propionic acid.
format	article
author	Taichi Ishikawa Daisuke Sasaki Ryo Aizawa Matsuo Yamamoto Takashi Yaegashi Tarou Irié Minoru Sasaki
author_facet	Taichi Ishikawa Daisuke Sasaki Ryo Aizawa Matsuo Yamamoto Takashi Yaegashi Tarou Irié Minoru Sasaki
author_sort	Taichi Ishikawa
title	The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease
title_short	The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease
title_full	The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease
title_fullStr	The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease
title_full_unstemmed	The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease
title_sort	role of lactic acid on wound healing, cell growth, cell cycle kinetics, and gene expression of cultured junctional epithelium cells in the pathophysiology of periodontal disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/19db7b3c362c487583d9b0d1515d0392
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The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease

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