DEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes

Abstract DEHP is a plasticizer which has been used in plastic products of everyday use for decades. Studies in mice and murine cell culture models identified DEHP as an endocrine disruptor that may also act as an obesogen. As this is of high concern in respect of the worldwide obesity epidemic, our...

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Autores principales:	Kristina Schaedlich, Scarlett Gebauer, Luise Hunger, Laura-Sophie Beier, Holger M. Koch, Martin Wabitsch, Bernd Fischer, Jana Ernst
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19e4938e1a2d4761a4caf9e55f68baa7
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spelling	oai:doaj.org-article:19e4938e1a2d4761a4caf9e55f68baa72021-12-02T12:32:35ZDEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes10.1038/s41598-018-21800-42045-2322https://doaj.org/article/19e4938e1a2d4761a4caf9e55f68baa72018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21800-4https://doaj.org/toc/2045-2322Abstract DEHP is a plasticizer which has been used in plastic products of everyday use for decades. Studies in mice and murine cell culture models identified DEHP as an endocrine disruptor that may also act as an obesogen. As this is of high concern in respect of the worldwide obesity epidemic, our aim is the translation of these findings into a human model system. On the basis of DOHaD, we investigated the influence of an environmentally relevant dose of DEHP [50 µg/ml] on adipogenesis in the human cell culture model SGBS. Pre-adipocytes were exposed to DEHP and differentiated into mature adipocytes. At different stages of differentiation, markers of adipogenesis like GLUT4, FABP4, LPL and PPARs, and of signaling pathways like AMPK/ACC2, JAK/STAT and MAPK were analyzed. Functional markers like adipokine secretion and triglyceride content as well as ROS production were measured in mature adipocytes. We found significantly lower expression levels of adipogenic markers, a reduction in lipid accumulation, higher leptin- and reduced adiponectin levels in the supernatant of treated adipocytes. Moreover, ROS production was significantly elevated after DEHP-exposure. In conclusion, DEHP led to lower grade of adipogenic differentiation in human SGBS-adipocytes under the chosen conditions.Kristina SchaedlichScarlett GebauerLuise HungerLaura-Sophie BeierHolger M. KochMartin WabitschBernd FischerJana ErnstNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristina Schaedlich Scarlett Gebauer Luise Hunger Laura-Sophie Beier Holger M. Koch Martin Wabitsch Bernd Fischer Jana Ernst DEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes
description	Abstract DEHP is a plasticizer which has been used in plastic products of everyday use for decades. Studies in mice and murine cell culture models identified DEHP as an endocrine disruptor that may also act as an obesogen. As this is of high concern in respect of the worldwide obesity epidemic, our aim is the translation of these findings into a human model system. On the basis of DOHaD, we investigated the influence of an environmentally relevant dose of DEHP [50 µg/ml] on adipogenesis in the human cell culture model SGBS. Pre-adipocytes were exposed to DEHP and differentiated into mature adipocytes. At different stages of differentiation, markers of adipogenesis like GLUT4, FABP4, LPL and PPARs, and of signaling pathways like AMPK/ACC2, JAK/STAT and MAPK were analyzed. Functional markers like adipokine secretion and triglyceride content as well as ROS production were measured in mature adipocytes. We found significantly lower expression levels of adipogenic markers, a reduction in lipid accumulation, higher leptin- and reduced adiponectin levels in the supernatant of treated adipocytes. Moreover, ROS production was significantly elevated after DEHP-exposure. In conclusion, DEHP led to lower grade of adipogenic differentiation in human SGBS-adipocytes under the chosen conditions.
format	article
author	Kristina Schaedlich Scarlett Gebauer Luise Hunger Laura-Sophie Beier Holger M. Koch Martin Wabitsch Bernd Fischer Jana Ernst
author_facet	Kristina Schaedlich Scarlett Gebauer Luise Hunger Laura-Sophie Beier Holger M. Koch Martin Wabitsch Bernd Fischer Jana Ernst
author_sort	Kristina Schaedlich
title	DEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes
title_short	DEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes
title_full	DEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes
title_fullStr	DEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes
title_full_unstemmed	DEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes
title_sort	dehp deregulates adipokine levels and impairs fatty acid storage in human sgbs-adipocytes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/19e4938e1a2d4761a4caf9e55f68baa7
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DEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes

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