The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model

Isothiocyanates (ITCs) show strong activity against numerous human tumors. Five structurally diverse ITCs were tested in vivo using the zebrafish embryos 6 and 48 h post-fertilization (hpf). The survival rate, hatching time, and gross morphological changes were assessed 24, 48, and 72 h after treatm...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Magdalena Rudzinska-Radecka, Łukasz Janczewski, Anna Gajda, Marlena Godlewska, Malgorzata Chmielewska-Krzesinska, Krzysztof Wasowicz, Piotr Podlasz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	zebrafish isothiocyanates glioblastoma cervical cancer breast cancer toxicology Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/19e6c9ce6874471dbd8f986aae26146d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:19e6c9ce6874471dbd8f986aae26146d
record_format	dspace
spelling	oai:doaj.org-article:19e6c9ce6874471dbd8f986aae26146d2021-11-25T17:12:59ZThe Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model10.3390/cells101132192073-4409https://doaj.org/article/19e6c9ce6874471dbd8f986aae26146d2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3219https://doaj.org/toc/2073-4409Isothiocyanates (ITCs) show strong activity against numerous human tumors. Five structurally diverse ITCs were tested in vivo using the zebrafish embryos 6 and 48 h post-fertilization (hpf). The survival rate, hatching time, and gross morphological changes were assessed 24, 48, and 72 h after treatment with all compounds in various doses (1–10 µM). As a result, we selected a phosphonate analog of sulforaphane (P-ITC; 1–3 µM) as a non-toxic treatment for zebrafish embryos, both 6 and 48 hpf. Furthermore, the in vivo anti-cancerogenic studies with selected 3 µM P-ITC were performed using a set of cell lines derived from the brain (U87), cervical (HeLa), and breast (MDA-MB-231) tumors. For the experiment, cells were labeled using red fluorescence dye Dil (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine, 10 μg/mL) and injected into the hindbrain ventricle, yolk sac region and Cuvier duct of zebrafish embryos. The tumor size measurement after 48 h of treatment demonstrated the significant inhibition of cancer cell growth in all tested cases by P-ITC compared to the non-treated controls. Our studies provided evidence for P-ITC anti-cancerogenic properties with versatile activity against different cancer types. Additionally, P-ITC demonstrated the safety of use in the living organism at various stages of embryogenesis.Magdalena Rudzinska-RadeckaŁukasz JanczewskiAnna GajdaMarlena GodlewskaMalgorzata Chmielewska-KrzesinskaKrzysztof WasowiczPiotr PodlaszMDPI AGarticlezebrafishisothiocyanatesglioblastomacervical cancerbreast cancertoxicologyBiology (General)QH301-705.5ENCells, Vol 10, Iss 3219, p 3219 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zebrafish isothiocyanates glioblastoma cervical cancer breast cancer toxicology Biology (General) QH301-705.5
spellingShingle	zebrafish isothiocyanates glioblastoma cervical cancer breast cancer toxicology Biology (General) QH301-705.5 Magdalena Rudzinska-Radecka Łukasz Janczewski Anna Gajda Marlena Godlewska Malgorzata Chmielewska-Krzesinska Krzysztof Wasowicz Piotr Podlasz The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model
description	Isothiocyanates (ITCs) show strong activity against numerous human tumors. Five structurally diverse ITCs were tested in vivo using the zebrafish embryos 6 and 48 h post-fertilization (hpf). The survival rate, hatching time, and gross morphological changes were assessed 24, 48, and 72 h after treatment with all compounds in various doses (1–10 µM). As a result, we selected a phosphonate analog of sulforaphane (P-ITC; 1–3 µM) as a non-toxic treatment for zebrafish embryos, both 6 and 48 hpf. Furthermore, the in vivo anti-cancerogenic studies with selected 3 µM P-ITC were performed using a set of cell lines derived from the brain (U87), cervical (HeLa), and breast (MDA-MB-231) tumors. For the experiment, cells were labeled using red fluorescence dye Dil (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine, 10 μg/mL) and injected into the hindbrain ventricle, yolk sac region and Cuvier duct of zebrafish embryos. The tumor size measurement after 48 h of treatment demonstrated the significant inhibition of cancer cell growth in all tested cases by P-ITC compared to the non-treated controls. Our studies provided evidence for P-ITC anti-cancerogenic properties with versatile activity against different cancer types. Additionally, P-ITC demonstrated the safety of use in the living organism at various stages of embryogenesis.
format	article
author	Magdalena Rudzinska-Radecka Łukasz Janczewski Anna Gajda Marlena Godlewska Malgorzata Chmielewska-Krzesinska Krzysztof Wasowicz Piotr Podlasz
author_facet	Magdalena Rudzinska-Radecka Łukasz Janczewski Anna Gajda Marlena Godlewska Malgorzata Chmielewska-Krzesinska Krzysztof Wasowicz Piotr Podlasz
author_sort	Magdalena Rudzinska-Radecka
title	The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model
title_short	The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model
title_full	The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model
title_fullStr	The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model
title_full_unstemmed	The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model
title_sort	anti-tumoral potential of phosphonate analog of sulforaphane in zebrafish xenograft model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/19e6c9ce6874471dbd8f986aae26146d
work_keys_str_mv	AT magdalenarudzinskaradecka theantitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT łukaszjanczewski theantitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT annagajda theantitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT marlenagodlewska theantitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT malgorzatachmielewskakrzesinska theantitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT krzysztofwasowicz theantitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT piotrpodlasz theantitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT magdalenarudzinskaradecka antitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT łukaszjanczewski antitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT annagajda antitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT marlenagodlewska antitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT malgorzatachmielewskakrzesinska antitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT krzysztofwasowicz antitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel AT piotrpodlasz antitumoralpotentialofphosphonateanalogofsulforaphaneinzebrafishxenograftmodel
_version_	1718412574082465792

The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model

Ejemplares similares