Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule

Diagnostics: Dual selection improves circulating tumor cell assay Using two selection markers, instead of one, can improve the sensitivity of a blood test for circulating tumor cells (CTCs). Steven A. Soper from the University of Kansas in Lawrence, USA, and colleagues analyzed blood samples from pa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Małgorzata A. Witek, Rachel D. Aufforth, Hong Wang, Joyce W. Kamande, Joshua M. Jackson, Swathi R. Pullagurla, Mateusz L. Hupert, Jerry Usary, Weiya Z. Wysham, Dawud Hilliard, Stephanie Montgomery, Victoria Bae-Jump, Lisa A. Carey, Paola A. Gehrig, Matthew I. Milowsky, Charles M. Perou, John T. Soper, Young E. Whang, Jen Jen Yeh, George Martin, Steven A. Soper
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/19e812126fd14335a0eee3c2e1583845
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:19e812126fd14335a0eee3c2e1583845
record_format	dspace
spelling	oai:doaj.org-article:19e812126fd14335a0eee3c2e15838452021-12-02T14:22:13ZDiscrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule10.1038/s41698-017-0028-82397-768Xhttps://doaj.org/article/19e812126fd14335a0eee3c2e15838452017-07-01T00:00:00Zhttps://doi.org/10.1038/s41698-017-0028-8https://doaj.org/toc/2397-768XDiagnostics: Dual selection improves circulating tumor cell assay Using two selection markers, instead of one, can improve the sensitivity of a blood test for circulating tumor cells (CTCs). Steven A. Soper from the University of Kansas in Lawrence, USA, and colleagues analyzed blood samples from patients with cancers of the pancreas, colon, breast, ovaries and prostate, as well blood from healthy donors and those with benign disease. They ran each patient’s blood through two microfluidic devices, one chip targeting the established marker epithelial cell adhesion molecule (EpCAM) and another targeting a new marker, fibroblast activation protein alpha (FAPα). Doing so detected CTCs in nearly all the cancer patients—a substantial improvement over testing for EpCAM CTCs alone. Molecular and genetic analyses of the CTCs showed that those expressing EpCAM and FAPα are distinct subpopulations, and testing for both could prove valuable for clinical management.Małgorzata A. WitekRachel D. AufforthHong WangJoyce W. KamandeJoshua M. JacksonSwathi R. PullagurlaMateusz L. HupertJerry UsaryWeiya Z. WyshamDawud HilliardStephanie MontgomeryVictoria Bae-JumpLisa A. CareyPaola A. GehrigMatthew I. MilowskyCharles M. PerouJohn T. SoperYoung E. WhangJen Jen YehGeorge MartinSteven A. SoperNature PortfolioarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Precision Oncology, Vol 1, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Małgorzata A. Witek Rachel D. Aufforth Hong Wang Joyce W. Kamande Joshua M. Jackson Swathi R. Pullagurla Mateusz L. Hupert Jerry Usary Weiya Z. Wysham Dawud Hilliard Stephanie Montgomery Victoria Bae-Jump Lisa A. Carey Paola A. Gehrig Matthew I. Milowsky Charles M. Perou John T. Soper Young E. Whang Jen Jen Yeh George Martin Steven A. Soper Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
description	Diagnostics: Dual selection improves circulating tumor cell assay Using two selection markers, instead of one, can improve the sensitivity of a blood test for circulating tumor cells (CTCs). Steven A. Soper from the University of Kansas in Lawrence, USA, and colleagues analyzed blood samples from patients with cancers of the pancreas, colon, breast, ovaries and prostate, as well blood from healthy donors and those with benign disease. They ran each patient’s blood through two microfluidic devices, one chip targeting the established marker epithelial cell adhesion molecule (EpCAM) and another targeting a new marker, fibroblast activation protein alpha (FAPα). Doing so detected CTCs in nearly all the cancer patients—a substantial improvement over testing for EpCAM CTCs alone. Molecular and genetic analyses of the CTCs showed that those expressing EpCAM and FAPα are distinct subpopulations, and testing for both could prove valuable for clinical management.
format	article
author	Małgorzata A. Witek Rachel D. Aufforth Hong Wang Joyce W. Kamande Joshua M. Jackson Swathi R. Pullagurla Mateusz L. Hupert Jerry Usary Weiya Z. Wysham Dawud Hilliard Stephanie Montgomery Victoria Bae-Jump Lisa A. Carey Paola A. Gehrig Matthew I. Milowsky Charles M. Perou John T. Soper Young E. Whang Jen Jen Yeh George Martin Steven A. Soper
author_facet	Małgorzata A. Witek Rachel D. Aufforth Hong Wang Joyce W. Kamande Joshua M. Jackson Swathi R. Pullagurla Mateusz L. Hupert Jerry Usary Weiya Z. Wysham Dawud Hilliard Stephanie Montgomery Victoria Bae-Jump Lisa A. Carey Paola A. Gehrig Matthew I. Milowsky Charles M. Perou John T. Soper Young E. Whang Jen Jen Yeh George Martin Steven A. Soper
author_sort	Małgorzata A. Witek
title	Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
title_short	Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
title_full	Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
title_fullStr	Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
title_full_unstemmed	Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
title_sort	discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/19e812126fd14335a0eee3c2e1583845
work_keys_str_mv	AT małgorzataawitek discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT racheldaufforth discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT hongwang discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT joycewkamande discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT joshuamjackson discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT swathirpullagurla discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT mateuszlhupert discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT jerryusary discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT weiyazwysham discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT dawudhilliard discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT stephaniemontgomery discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT victoriabaejump discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT lisaacarey discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT paolaagehrig discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT matthewimilowsky discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT charlesmperou discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT johntsoper discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT youngewhang discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT jenjenyeh discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT georgemartin discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule AT stevenasoper discretemicrofluidicsfortheisolationofcirculatingtumorcellsubpopulationstargetingfibroblastactivationproteinalphaandepithelialcelladhesionmolecule
_version_	1718391471357296640

Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule

Ejemplares similares