Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker

Abstract. Barus T, Halim R, Hartanti AT, Saputra PK. 2019. Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker. Biodiversitas 20: 847-852. The main microorganism for tempeh fermentation is Rhizopus microsporus. These days,...

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Autores principales:	TATI BARUS, RONALDO HALIM, ANASTASIA TATIK HARTANTI, PAULUS KEVIN SAPUTRA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/19ef2ed2a04c42608bfb6b36edaae28d
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spelling	oai:doaj.org-article:19ef2ed2a04c42608bfb6b36edaae28d2021-11-16T14:12:14ZGenetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker1412-033X2085-472210.13057/biodiv/d200331https://doaj.org/article/19ef2ed2a04c42608bfb6b36edaae28d2019-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3277https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Barus T, Halim R, Hartanti AT, Saputra PK. 2019. Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker. Biodiversitas 20: 847-852. The main microorganism for tempeh fermentation is Rhizopus microsporus. These days, many tempeh producers use commercial inoculum, such as ‘Raprima’ as resource of R. microsporus. As a result, the genetic diversity of R. microsporus that had been reported in Indonesia has diminished. Information about genetic diversity is needed as a basis to select R. microsporus as tempeh inoculum. This research aims to investigate the genetic diversity of R. microsporus from waru leaves based on Internal Transcribed Spacer (ITS) Sequence and Random Amplified Polymorphic DNA (RAPD) markers. A total of 25 R. microsporus were isolated from traditional inoculum waru leaves (Inoculum 1) and traditional inoculum other than waru leaves (Inoculum 2). Amplification of ITS sequence was done using universal primer pairs of ITS-4 and ITS-5. Amplification of RAPD markers was done using primers OPC-08, OPC-19, OPQ-6, R-108, OPA-09 and OPJ-20. ITS sequence was not sufficient to compare the similarities among R. microsporus. On the other hand, RAPD markers successfully compared the similarities among 25 R. microsporus. A total of 25 R. microsporus were divided into 9 clusters. R. microsporus from Inoculum 1 grouped into Cluster 1, Cluster 3 and Cluster 4-8. Inoculum 2 grouped into Cluster 2 and Cluster 9. R. microsporus from tempeh grouped into Cluster 4 and was different from Inoculum 1 and Inoculum 2, except for TB3.TATI BARUSRONALDO HALIMANASTASIA TATIK HARTANTIPAULUS KEVIN SAPUTRAMBI & UNS SoloarticleBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 3, Pp 847-852 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 TATI BARUS RONALDO HALIM ANASTASIA TATIK HARTANTI PAULUS KEVIN SAPUTRA Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker
description	Abstract. Barus T, Halim R, Hartanti AT, Saputra PK. 2019. Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker. Biodiversitas 20: 847-852. The main microorganism for tempeh fermentation is Rhizopus microsporus. These days, many tempeh producers use commercial inoculum, such as ‘Raprima’ as resource of R. microsporus. As a result, the genetic diversity of R. microsporus that had been reported in Indonesia has diminished. Information about genetic diversity is needed as a basis to select R. microsporus as tempeh inoculum. This research aims to investigate the genetic diversity of R. microsporus from waru leaves based on Internal Transcribed Spacer (ITS) Sequence and Random Amplified Polymorphic DNA (RAPD) markers. A total of 25 R. microsporus were isolated from traditional inoculum waru leaves (Inoculum 1) and traditional inoculum other than waru leaves (Inoculum 2). Amplification of ITS sequence was done using universal primer pairs of ITS-4 and ITS-5. Amplification of RAPD markers was done using primers OPC-08, OPC-19, OPQ-6, R-108, OPA-09 and OPJ-20. ITS sequence was not sufficient to compare the similarities among R. microsporus. On the other hand, RAPD markers successfully compared the similarities among 25 R. microsporus. A total of 25 R. microsporus were divided into 9 clusters. R. microsporus from Inoculum 1 grouped into Cluster 1, Cluster 3 and Cluster 4-8. Inoculum 2 grouped into Cluster 2 and Cluster 9. R. microsporus from tempeh grouped into Cluster 4 and was different from Inoculum 1 and Inoculum 2, except for TB3.
format	article
author	TATI BARUS RONALDO HALIM ANASTASIA TATIK HARTANTI PAULUS KEVIN SAPUTRA
author_facet	TATI BARUS RONALDO HALIM ANASTASIA TATIK HARTANTI PAULUS KEVIN SAPUTRA
author_sort	TATI BARUS
title	Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker
title_short	Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker
title_full	Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker
title_fullStr	Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker
title_full_unstemmed	Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker
title_sort	genetic diversity of rhizopus microsporus from traditional inoculum of tempeh in indonesia based on its sequences and rapd marker
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/19ef2ed2a04c42608bfb6b36edaae28d
work_keys_str_mv	AT tatibarus geneticdiversityofrhizopusmicrosporusfromtraditionalinoculumoftempehinindonesiabasedonitssequencesandrapdmarker AT ronaldohalim geneticdiversityofrhizopusmicrosporusfromtraditionalinoculumoftempehinindonesiabasedonitssequencesandrapdmarker AT anastasiatatikhartanti geneticdiversityofrhizopusmicrosporusfromtraditionalinoculumoftempehinindonesiabasedonitssequencesandrapdmarker AT pauluskevinsaputra geneticdiversityofrhizopusmicrosporusfromtraditionalinoculumoftempehinindonesiabasedonitssequencesandrapdmarker
_version_	1718426344969207808

Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker

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