Annexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.

Annexin A2 (ANXA2) is a protein implicated in diverse cellular functions, including exocytosis, DNA synthesis and cell proliferation. It was recently proposed to be involved in RNA metabolism because it was shown to associate with some cellular mRNA. Here, we identified ANXA2 as a RNA binding protei...

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Autores principales:	Hoyun Kwak, Min Woo Park, Sunjoo Jeong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19f83dc332574aeda2ffb99ffda48a50
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spelling	oai:doaj.org-article:19f83dc332574aeda2ffb99ffda48a502021-11-18T06:47:01ZAnnexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.1932-620310.1371/journal.pone.0024067https://doaj.org/article/19f83dc332574aeda2ffb99ffda48a502011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21918681/?tool=EBIhttps://doaj.org/toc/1932-6203Annexin A2 (ANXA2) is a protein implicated in diverse cellular functions, including exocytosis, DNA synthesis and cell proliferation. It was recently proposed to be involved in RNA metabolism because it was shown to associate with some cellular mRNA. Here, we identified ANXA2 as a RNA binding protein (RBP) that binds IBV (Infectious Bronchitis Virus) pseudoknot RNA. We first confirmed the binding of ANXA2 to IBV pseudoknot RNA by ultraviolet crosslinking and showed its binding to RNA pseudoknot with ANXA2 protein in vitro and in the cells. Since the RNA pseudoknot located in the frameshifting region of IBV was used as bait for cellular RBPs, we tested whether ANXA2 could regulate the frameshfting of IBV pseudoknot RNA by dual luciferase assay. Overexpression of ANXA2 significantly reduced the frameshifting efficiency from IBV pseudoknot RNA and knockdown of the protein strikingly increased the frameshifting efficiency. The results suggest that ANXA2 is a cellular RBP that can modulate the frameshifting efficiency of viral RNA, enabling it to act as an anti-viral cellular protein, and hinting at roles in RNA metabolism for other cellular mRNAs.Hoyun KwakMin Woo ParkSunjoo JeongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e24067 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hoyun Kwak Min Woo Park Sunjoo Jeong Annexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.
description	Annexin A2 (ANXA2) is a protein implicated in diverse cellular functions, including exocytosis, DNA synthesis and cell proliferation. It was recently proposed to be involved in RNA metabolism because it was shown to associate with some cellular mRNA. Here, we identified ANXA2 as a RNA binding protein (RBP) that binds IBV (Infectious Bronchitis Virus) pseudoknot RNA. We first confirmed the binding of ANXA2 to IBV pseudoknot RNA by ultraviolet crosslinking and showed its binding to RNA pseudoknot with ANXA2 protein in vitro and in the cells. Since the RNA pseudoknot located in the frameshifting region of IBV was used as bait for cellular RBPs, we tested whether ANXA2 could regulate the frameshfting of IBV pseudoknot RNA by dual luciferase assay. Overexpression of ANXA2 significantly reduced the frameshifting efficiency from IBV pseudoknot RNA and knockdown of the protein strikingly increased the frameshifting efficiency. The results suggest that ANXA2 is a cellular RBP that can modulate the frameshifting efficiency of viral RNA, enabling it to act as an anti-viral cellular protein, and hinting at roles in RNA metabolism for other cellular mRNAs.
format	article
author	Hoyun Kwak Min Woo Park Sunjoo Jeong
author_facet	Hoyun Kwak Min Woo Park Sunjoo Jeong
author_sort	Hoyun Kwak
title	Annexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.
title_short	Annexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.
title_full	Annexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.
title_fullStr	Annexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.
title_full_unstemmed	Annexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.
title_sort	annexin a2 binds rna and reduces the frameshifting efficiency of infectious bronchitis virus.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/19f83dc332574aeda2ffb99ffda48a50
work_keys_str_mv	AT hoyunkwak annexina2bindsrnaandreducestheframeshiftingefficiencyofinfectiousbronchitisvirus AT minwoopark annexina2bindsrnaandreducestheframeshiftingefficiencyofinfectiousbronchitisvirus AT sunjoojeong annexina2bindsrnaandreducestheframeshiftingefficiencyofinfectiousbronchitisvirus
_version_	1718424400607313920

Annexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.

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