Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)

Abstract Background Insulin-like growth factor 2 (IGF2) is one of three hormones that share high structural similarity to insulin. It is involved in several insulin-like growth-regulating and mitogenic activities. This study was conducted to genotype the coding regions of the IGF2 gene in Japanese q...

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Autores principales:	Dhafer A. Ali, Mohammed Baqur S. Al-Shuhaib, Golzar Farhadi, Fadhil R. Al-Kafajy, Tahreer M. Al-Thuwaini, Ali Esmailizadeh
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Eggs production Gene variation IGF2 Japanese quails Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/19f91cf240394bcaa39b41c40872b2bd
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spelling	oai:doaj.org-article:19f91cf240394bcaa39b41c40872b2bd2021-11-08T11:04:27ZDetection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)10.1186/s43141-021-00271-72090-5920https://doaj.org/article/19f91cf240394bcaa39b41c40872b2bd2021-11-01T00:00:00Zhttps://doi.org/10.1186/s43141-021-00271-7https://doaj.org/toc/2090-5920Abstract Background Insulin-like growth factor 2 (IGF2) is one of three hormones that share high structural similarity to insulin. It is involved in several insulin-like growth-regulating and mitogenic activities. This study was conducted to genotype the coding regions of the IGF2 gene in Japanese quail (Coturnix japonica) using PCR-SSCP-sequencing, and to assess the possible association of the polymorphism of these regions with the main egg production traits. A total of 240 female birds with an equal number of three Japanese quail populations (Brown or BR, Black or BL, and White or WT) were included in this study. Results All the genotyped regions exerted no heterogeneity in their sequences with one exception detected in the exon 2. In this locus, a novel single nucleotide polymorphism (SNP) was detected in which “A” was substituted with “G” at 81 position with a silent effect (p.F79=SNP) on IGF2 protein. Association analyses indicated a significant (P < 0.05) relation of this SNP with egg number (EN) and bird weight (BW) in the analyzed populations, in which the birds with AG genotype had lower EN and BW than those with AA genotype. The p.F79=SNP was largely detected in the WT line than the other two lines. Conclusion The detected p.F79=SNP has a highly negative effect on EN and BW in Japanese quail. Thus, the implementation of the variations of the IGF2 gene can be a useful marker in the marker-assisted selection of Japanese quail. This is the first report to describe IGF2 gene variations in Japanese quail, which strongly suggests raising the birds from the BR line with AA genotype when breeders desire to raise Japanese quail for large-scale egg production.Dhafer A. AliMohammed Baqur S. Al-ShuhaibGolzar FarhadiFadhil R. Al-KafajyTahreer M. Al-ThuwainiAli EsmailizadehSpringerOpenarticleEggs productionGene variationIGF2Japanese quailsBiotechnologyTP248.13-248.65GeneticsQH426-470ENJournal of Genetic Engineering and Biotechnology, Vol 19, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Eggs production Gene variation IGF2 Japanese quails Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Eggs production Gene variation IGF2 Japanese quails Biotechnology TP248.13-248.65 Genetics QH426-470 Dhafer A. Ali Mohammed Baqur S. Al-Shuhaib Golzar Farhadi Fadhil R. Al-Kafajy Tahreer M. Al-Thuwaini Ali Esmailizadeh Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)
description	Abstract Background Insulin-like growth factor 2 (IGF2) is one of three hormones that share high structural similarity to insulin. It is involved in several insulin-like growth-regulating and mitogenic activities. This study was conducted to genotype the coding regions of the IGF2 gene in Japanese quail (Coturnix japonica) using PCR-SSCP-sequencing, and to assess the possible association of the polymorphism of these regions with the main egg production traits. A total of 240 female birds with an equal number of three Japanese quail populations (Brown or BR, Black or BL, and White or WT) were included in this study. Results All the genotyped regions exerted no heterogeneity in their sequences with one exception detected in the exon 2. In this locus, a novel single nucleotide polymorphism (SNP) was detected in which “A” was substituted with “G” at 81 position with a silent effect (p.F79=SNP) on IGF2 protein. Association analyses indicated a significant (P < 0.05) relation of this SNP with egg number (EN) and bird weight (BW) in the analyzed populations, in which the birds with AG genotype had lower EN and BW than those with AA genotype. The p.F79=SNP was largely detected in the WT line than the other two lines. Conclusion The detected p.F79=SNP has a highly negative effect on EN and BW in Japanese quail. Thus, the implementation of the variations of the IGF2 gene can be a useful marker in the marker-assisted selection of Japanese quail. This is the first report to describe IGF2 gene variations in Japanese quail, which strongly suggests raising the birds from the BR line with AA genotype when breeders desire to raise Japanese quail for large-scale egg production.
format	article
author	Dhafer A. Ali Mohammed Baqur S. Al-Shuhaib Golzar Farhadi Fadhil R. Al-Kafajy Tahreer M. Al-Thuwaini Ali Esmailizadeh
author_facet	Dhafer A. Ali Mohammed Baqur S. Al-Shuhaib Golzar Farhadi Fadhil R. Al-Kafajy Tahreer M. Al-Thuwaini Ali Esmailizadeh
author_sort	Dhafer A. Ali
title	Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)
title_short	Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)
title_full	Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)
title_fullStr	Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)
title_full_unstemmed	Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)
title_sort	detection of a novel single nucleotide polymorphism in igf2 gene with a negative impact on egg production and body weight in japanese quail (coturnix japonica)
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/19f91cf240394bcaa39b41c40872b2bd
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Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)

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