Plant Extract of <i>Limonium gmelinii</i> Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion

There are numerous publications demonstrating that plant polyphenols can reduce oxidative stress and inflammatory processes in the brain. In the present study we have investigated the neuroprotective effect of plant extract isolated from the roots of <i>L. gmelinii</i> since it contains...

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Autores principales:	Tulendy Nurkenov, Andrey Tsoy, Farkhad Olzhayev, Elvira Abzhanova, Anel Turgambayeva, Aizhan Zhussupova, Bharathi Avula, Samir Ross, Aigerim Aituarova, Dariya Kassymova, Galiya Zhusupova, Tamara Shalakhmetova, Tursonjan Tokay, James C. Lee, Sholpan Askarova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antioxidants neurons astrocytes CECs <i>L. gmelinii</i> plant polyphenols Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/1a0033f83fe346a1b2bf9a6759a83f36
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spelling	oai:doaj.org-article:1a0033f83fe346a1b2bf9a6759a83f362021-11-25T16:28:57ZPlant Extract of <i>Limonium gmelinii</i> Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion10.3390/antiox101118142076-3921https://doaj.org/article/1a0033f83fe346a1b2bf9a6759a83f362021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1814https://doaj.org/toc/2076-3921There are numerous publications demonstrating that plant polyphenols can reduce oxidative stress and inflammatory processes in the brain. In the present study we have investigated the neuroprotective effect of plant extract isolated from the roots of <i>L. gmelinii</i> since it contains a rich source of polyphenols and other biologically active compounds. We have applied an oxidative and inflammatory model induced by NMDA, H<sub>2</sub>O<sub>2</sub>, and TNF-α in human primary neurons and astrocytes, and mouse cerebral endothelial cell (CECs) line in vitro. The levels of ROS generation, NADPH oxidase activation, P-selectin expression, and activity of ERK1/2 were evaluated by quantitative immunofluorescence analysis, confocal microscopy, and MAPK assay. In vivo, sensorimotor functions in rats with middle cerebral artery occlusion (MCAO) were assessed. In neurons NMDA induced overproduction of ROS, in astrocytes TNF-α initiated ROS generation, NADPH oxidase activation, and phosphorylation of ERK1/2. In CECs, the exposure by TNF-α induced oxidative stress and triggered the accumulation of P-selectin on the surface of the cells. In turn, pre-treatment of the cells with the extract of <i>L. gmelinii</i> suppressed oxidative stress in all cell types and pro-inflammatory responses in astrocytes and CECs. In vivo, the treatment with <i>L. gmelinii</i> extract improved motor activity in rats with MCAO.Tulendy NurkenovAndrey TsoyFarkhad OlzhayevElvira AbzhanovaAnel TurgambayevaAizhan ZhussupovaBharathi AvulaSamir RossAigerim AituarovaDariya KassymovaGaliya ZhusupovaTamara ShalakhmetovaTursonjan TokayJames C. LeeSholpan AskarovaMDPI AGarticleantioxidantsneuronsastrocytesCECs<i>L. gmelinii</i>plant polyphenolsTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1814, p 1814 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidants neurons astrocytes CECs <i>L. gmelinii</i> plant polyphenols Therapeutics. Pharmacology RM1-950
spellingShingle	antioxidants neurons astrocytes CECs <i>L. gmelinii</i> plant polyphenols Therapeutics. Pharmacology RM1-950 Tulendy Nurkenov Andrey Tsoy Farkhad Olzhayev Elvira Abzhanova Anel Turgambayeva Aizhan Zhussupova Bharathi Avula Samir Ross Aigerim Aituarova Dariya Kassymova Galiya Zhusupova Tamara Shalakhmetova Tursonjan Tokay James C. Lee Sholpan Askarova Plant Extract of <i>Limonium gmelinii</i> Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion
description	There are numerous publications demonstrating that plant polyphenols can reduce oxidative stress and inflammatory processes in the brain. In the present study we have investigated the neuroprotective effect of plant extract isolated from the roots of <i>L. gmelinii</i> since it contains a rich source of polyphenols and other biologically active compounds. We have applied an oxidative and inflammatory model induced by NMDA, H<sub>2</sub>O<sub>2</sub>, and TNF-α in human primary neurons and astrocytes, and mouse cerebral endothelial cell (CECs) line in vitro. The levels of ROS generation, NADPH oxidase activation, P-selectin expression, and activity of ERK1/2 were evaluated by quantitative immunofluorescence analysis, confocal microscopy, and MAPK assay. In vivo, sensorimotor functions in rats with middle cerebral artery occlusion (MCAO) were assessed. In neurons NMDA induced overproduction of ROS, in astrocytes TNF-α initiated ROS generation, NADPH oxidase activation, and phosphorylation of ERK1/2. In CECs, the exposure by TNF-α induced oxidative stress and triggered the accumulation of P-selectin on the surface of the cells. In turn, pre-treatment of the cells with the extract of <i>L. gmelinii</i> suppressed oxidative stress in all cell types and pro-inflammatory responses in astrocytes and CECs. In vivo, the treatment with <i>L. gmelinii</i> extract improved motor activity in rats with MCAO.
format	article
author	Tulendy Nurkenov Andrey Tsoy Farkhad Olzhayev Elvira Abzhanova Anel Turgambayeva Aizhan Zhussupova Bharathi Avula Samir Ross Aigerim Aituarova Dariya Kassymova Galiya Zhusupova Tamara Shalakhmetova Tursonjan Tokay James C. Lee Sholpan Askarova
author_facet	Tulendy Nurkenov Andrey Tsoy Farkhad Olzhayev Elvira Abzhanova Anel Turgambayeva Aizhan Zhussupova Bharathi Avula Samir Ross Aigerim Aituarova Dariya Kassymova Galiya Zhusupova Tamara Shalakhmetova Tursonjan Tokay James C. Lee Sholpan Askarova
author_sort	Tulendy Nurkenov
title	Plant Extract of <i>Limonium gmelinii</i> Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion
title_short	Plant Extract of <i>Limonium gmelinii</i> Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion
title_full	Plant Extract of <i>Limonium gmelinii</i> Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion
title_fullStr	Plant Extract of <i>Limonium gmelinii</i> Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion
title_full_unstemmed	Plant Extract of <i>Limonium gmelinii</i> Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion
title_sort	plant extract of <i>limonium gmelinii</i> attenuates oxidative responses in neurons, astrocytes, and cerebral endothelial cells in vitro and improves motor functions of rats after middle cerebral artery occlusion
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1a0033f83fe346a1b2bf9a6759a83f36
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Plant Extract of <i>Limonium gmelinii</i> Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion

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