An Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs

ABSTRACT This technical report describes the development of an aptamer for sensing azole antifungal drugs during therapeutic drug monitoring. Modified synthetic evolution of ligands through exponential enrichment (SELEX) was used to discover a DNA aptamer recognizing azole class antifungal drugs. Th...

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Autores principales:	Gregory R. Wiedman, Yanan Zhao, Arkady Mustaev, Jinglei Ping, Ramya Vishnubhotla, A. T. Charlie Johnson, David S. Perlin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	antifungals aptamers graphene biosensors Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1a0982c96d9845778937c111a453bd6f
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spelling	oai:doaj.org-article:1a0982c96d9845778937c111a453bd6f2021-11-15T15:22:04ZAn Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs10.1128/mSphere.00274-172379-5042https://doaj.org/article/1a0982c96d9845778937c111a453bd6f2017-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00274-17https://doaj.org/toc/2379-5042ABSTRACT This technical report describes the development of an aptamer for sensing azole antifungal drugs during therapeutic drug monitoring. Modified synthetic evolution of ligands through exponential enrichment (SELEX) was used to discover a DNA aptamer recognizing azole class antifungal drugs. This aptamer undergoes a secondary structural change upon binding to its target molecule, as shown through fluorescence anisotropy-based binding measurements. Experiments using circular dichroism spectroscopy revealed a unique G-quadruplex structure that was essential and specific for binding to the azole antifungal target. Aptamer-functionalized graphene field effect transistor (GFET) devices were created and used to measure the strength of binding of azole antifungals to this surface. In total, this aptamer and the supporting sensing platform provide a valuable tool for therapeutic drug monitoring of patients with invasive fungal infections. IMPORTANCE We have developed the first aptamer directed toward the azole class of antifungal drugs and a functional biosensor for these drugs. This aptamer has a unique secondary structure that allows it to bind to highly hydrophobic drugs. The aptamer works as a capture component of a graphene field effect transistor device. These devices can provide a quick and easy assay for determining drug concentrations. These will be useful for therapeutic drug monitoring of azole antifungal drugs, which is necessary to deal with the complex drug dosage profiles.Gregory R. WiedmanYanan ZhaoArkady MustaevJinglei PingRamya VishnubhotlaA. T. Charlie JohnsonDavid S. PerlinAmerican Society for MicrobiologyarticleantifungalsaptamersgraphenebiosensorsMicrobiologyQR1-502ENmSphere, Vol 2, Iss 4 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	antifungals aptamers graphene biosensors Microbiology QR1-502
spellingShingle	antifungals aptamers graphene biosensors Microbiology QR1-502 Gregory R. Wiedman Yanan Zhao Arkady Mustaev Jinglei Ping Ramya Vishnubhotla A. T. Charlie Johnson David S. Perlin An Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs
description	ABSTRACT This technical report describes the development of an aptamer for sensing azole antifungal drugs during therapeutic drug monitoring. Modified synthetic evolution of ligands through exponential enrichment (SELEX) was used to discover a DNA aptamer recognizing azole class antifungal drugs. This aptamer undergoes a secondary structural change upon binding to its target molecule, as shown through fluorescence anisotropy-based binding measurements. Experiments using circular dichroism spectroscopy revealed a unique G-quadruplex structure that was essential and specific for binding to the azole antifungal target. Aptamer-functionalized graphene field effect transistor (GFET) devices were created and used to measure the strength of binding of azole antifungals to this surface. In total, this aptamer and the supporting sensing platform provide a valuable tool for therapeutic drug monitoring of patients with invasive fungal infections. IMPORTANCE We have developed the first aptamer directed toward the azole class of antifungal drugs and a functional biosensor for these drugs. This aptamer has a unique secondary structure that allows it to bind to highly hydrophobic drugs. The aptamer works as a capture component of a graphene field effect transistor device. These devices can provide a quick and easy assay for determining drug concentrations. These will be useful for therapeutic drug monitoring of azole antifungal drugs, which is necessary to deal with the complex drug dosage profiles.
format	article
author	Gregory R. Wiedman Yanan Zhao Arkady Mustaev Jinglei Ping Ramya Vishnubhotla A. T. Charlie Johnson David S. Perlin
author_facet	Gregory R. Wiedman Yanan Zhao Arkady Mustaev Jinglei Ping Ramya Vishnubhotla A. T. Charlie Johnson David S. Perlin
author_sort	Gregory R. Wiedman
title	An Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs
title_short	An Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs
title_full	An Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs
title_fullStr	An Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs
title_full_unstemmed	An Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs
title_sort	aptamer-based biosensor for the azole class of antifungal drugs
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/1a0982c96d9845778937c111a453bd6f
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An Aptamer-Based Biosensor for the Azole Class of Antifungal Drugs

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