Microbial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration

Abstract Mining of mineral resources produces substantial volumes of crushed rock based wastes that are characterised by poor physical structure and hydrology, unstable geochemistry and potentially toxic chemical conditions. Recycling of these substrates is desirable and can be achieved by blending...

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Autores principales:	Deepak Kumaresan, Adam T Cross, Benjamin Moreira-Grez, Khalil Kariman, Paul Nevill, Jason Stevens, Richard J N Allcock, Anthony G O’Donnell, Kingsley W Dixon, Andrew S Whiteley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a099952c39f4fdeae96f1d57671b9be
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spelling	oai:doaj.org-article:1a099952c39f4fdeae96f1d57671b9be2021-12-02T16:06:05ZMicrobial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration10.1038/s41598-017-00650-62045-2322https://doaj.org/article/1a099952c39f4fdeae96f1d57671b9be2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00650-6https://doaj.org/toc/2045-2322Abstract Mining of mineral resources produces substantial volumes of crushed rock based wastes that are characterised by poor physical structure and hydrology, unstable geochemistry and potentially toxic chemical conditions. Recycling of these substrates is desirable and can be achieved by blending waste with native soil to form a ‘novel substrate’ which may be used in future landscape restoration. However, these post-mining substrate based ‘soils’ are likely to contain significant abiotic constraints for both plant and microbial growth. Effective use of these novel substrates for ecosystem restoration will depend on the efficacy of stored topsoil as a potential microbial inoculum as well as the subsequent generation of key microbial soil functions originally apparent in local pristine sites. Here, using both marker gene and shotgun metagenome sequencing, we show that topsoil storage and the blending of soil and waste substrates to form planting substrates gives rise to variable bacterial and archaeal phylogenetic composition but a high degree of metabolic conservation at the community metagenome level. Our data indicates that whilst low phylogenetic conservation is apparent across substrate blends we observe high functional redundancy in relation to key soil microbial pathways, allowing the potential for functional recovery of key belowground pathways under targeted management.Deepak KumaresanAdam T CrossBenjamin Moreira-GrezKhalil KarimanPaul NevillJason StevensRichard J N AllcockAnthony G O’DonnellKingsley W DixonAndrew S WhiteleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Deepak Kumaresan Adam T Cross Benjamin Moreira-Grez Khalil Kariman Paul Nevill Jason Stevens Richard J N Allcock Anthony G O’Donnell Kingsley W Dixon Andrew S Whiteley Microbial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration
description	Abstract Mining of mineral resources produces substantial volumes of crushed rock based wastes that are characterised by poor physical structure and hydrology, unstable geochemistry and potentially toxic chemical conditions. Recycling of these substrates is desirable and can be achieved by blending waste with native soil to form a ‘novel substrate’ which may be used in future landscape restoration. However, these post-mining substrate based ‘soils’ are likely to contain significant abiotic constraints for both plant and microbial growth. Effective use of these novel substrates for ecosystem restoration will depend on the efficacy of stored topsoil as a potential microbial inoculum as well as the subsequent generation of key microbial soil functions originally apparent in local pristine sites. Here, using both marker gene and shotgun metagenome sequencing, we show that topsoil storage and the blending of soil and waste substrates to form planting substrates gives rise to variable bacterial and archaeal phylogenetic composition but a high degree of metabolic conservation at the community metagenome level. Our data indicates that whilst low phylogenetic conservation is apparent across substrate blends we observe high functional redundancy in relation to key soil microbial pathways, allowing the potential for functional recovery of key belowground pathways under targeted management.
format	article
author	Deepak Kumaresan Adam T Cross Benjamin Moreira-Grez Khalil Kariman Paul Nevill Jason Stevens Richard J N Allcock Anthony G O’Donnell Kingsley W Dixon Andrew S Whiteley
author_facet	Deepak Kumaresan Adam T Cross Benjamin Moreira-Grez Khalil Kariman Paul Nevill Jason Stevens Richard J N Allcock Anthony G O’Donnell Kingsley W Dixon Andrew S Whiteley
author_sort	Deepak Kumaresan
title	Microbial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration
title_short	Microbial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration
title_full	Microbial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration
title_fullStr	Microbial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration
title_full_unstemmed	Microbial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration
title_sort	microbial functional capacity is preserved within engineered soil formulations used in mine site restoration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1a099952c39f4fdeae96f1d57671b9be
work_keys_str_mv	AT deepakkumaresan microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration AT adamtcross microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration AT benjaminmoreiragrez microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration AT khalilkariman microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration AT paulnevill microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration AT jasonstevens microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration AT richardjnallcock microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration AT anthonygodonnell microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration AT kingsleywdixon microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration AT andrewswhiteley microbialfunctionalcapacityispreservedwithinengineeredsoilformulationsusedinminesiterestoration
_version_	1718385073138434048

Microbial Functional Capacity Is Preserved Within Engineered Soil Formulations Used In Mine Site Restoration

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