Partial Disruption of Translational and Posttranslational Machinery Reshapes Growth Rates of <named-content content-type="genus-species">Bartonella birtlesii</named-content>

ABSTRACT Specialization of bacteria in a new niche is associated with genome repertoire changes, and speciation in bacterial specialists is associated with genome reduction. Here, we tested a signature-tagged mutant library of 3,456 Bartonella birtlesii clones to detect mutants that could grow rapid...

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Autores principales:	Jean Marc Rolain, Muriel Vayssier-Taussat, Watcharee Saisongkorh, Vicky Merhej, Gregory Gimenez, Catherine Robert, Danielle Le Rhun, Christoph Dehio, Didier Raoult
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1a09dd9faee8469eb18f2a3917ab1cb5
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spelling	oai:doaj.org-article:1a09dd9faee8469eb18f2a3917ab1cb52021-11-15T15:40:27ZPartial Disruption of Translational and Posttranslational Machinery Reshapes Growth Rates of <named-content content-type="genus-species">Bartonella birtlesii</named-content>10.1128/mBio.00115-132150-7511https://doaj.org/article/1a09dd9faee8469eb18f2a3917ab1cb52013-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00115-13https://doaj.org/toc/2150-7511ABSTRACT Specialization of bacteria in a new niche is associated with genome repertoire changes, and speciation in bacterial specialists is associated with genome reduction. Here, we tested a signature-tagged mutant library of 3,456 Bartonella birtlesii clones to detect mutants that could grow rapidly in vitro. Overall, we found 124 mutants that grew faster than the parental wild-type strain in vitro. We sequenced the genomes of the four mutants with the most rapid growth (formed visible colonies in only 1 to 2 days compared with 5 days for the wild type) and compared them to the parental isolate genome. We found that the number of disrupted genes associated with translation in the 124 rapid-growth clones was significantly higher than the number of genes involved in translation in the full genome (P < 10−6). Analysis of transposon integration in the genome of the four most rapidly growing clones revealed that one clone lacked one of the two wild-type RNA ribosomal operons. Finally, one of the four clones did not induce bacteremia in our mouse model, whereas infection with the other three resulted in a significantly lower bacterial count in blood than that with the wild-type strain. IMPORTANCE Here, we show that specialization in a specific niche could be caused by the disruption of critical genes. Most of these genes were involved in translation, and we show that evolution of obligate parasitism bacteria was specifically associated with disruption of translation system-encoding genes.Jean Marc RolainMuriel Vayssier-TaussatWatcharee SaisongkorhVicky MerhejGregory GimenezCatherine RobertDanielle Le RhunChristoph DehioDidier RaoultAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 2 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Jean Marc Rolain Muriel Vayssier-Taussat Watcharee Saisongkorh Vicky Merhej Gregory Gimenez Catherine Robert Danielle Le Rhun Christoph Dehio Didier Raoult Partial Disruption of Translational and Posttranslational Machinery Reshapes Growth Rates of <named-content content-type="genus-species">Bartonella birtlesii</named-content>
description	ABSTRACT Specialization of bacteria in a new niche is associated with genome repertoire changes, and speciation in bacterial specialists is associated with genome reduction. Here, we tested a signature-tagged mutant library of 3,456 Bartonella birtlesii clones to detect mutants that could grow rapidly in vitro. Overall, we found 124 mutants that grew faster than the parental wild-type strain in vitro. We sequenced the genomes of the four mutants with the most rapid growth (formed visible colonies in only 1 to 2 days compared with 5 days for the wild type) and compared them to the parental isolate genome. We found that the number of disrupted genes associated with translation in the 124 rapid-growth clones was significantly higher than the number of genes involved in translation in the full genome (P < 10−6). Analysis of transposon integration in the genome of the four most rapidly growing clones revealed that one clone lacked one of the two wild-type RNA ribosomal operons. Finally, one of the four clones did not induce bacteremia in our mouse model, whereas infection with the other three resulted in a significantly lower bacterial count in blood than that with the wild-type strain. IMPORTANCE Here, we show that specialization in a specific niche could be caused by the disruption of critical genes. Most of these genes were involved in translation, and we show that evolution of obligate parasitism bacteria was specifically associated with disruption of translation system-encoding genes.
format	article
author	Jean Marc Rolain Muriel Vayssier-Taussat Watcharee Saisongkorh Vicky Merhej Gregory Gimenez Catherine Robert Danielle Le Rhun Christoph Dehio Didier Raoult
author_facet	Jean Marc Rolain Muriel Vayssier-Taussat Watcharee Saisongkorh Vicky Merhej Gregory Gimenez Catherine Robert Danielle Le Rhun Christoph Dehio Didier Raoult
author_sort	Jean Marc Rolain
title	Partial Disruption of Translational and Posttranslational Machinery Reshapes Growth Rates of <named-content content-type="genus-species">Bartonella birtlesii</named-content>
title_short	Partial Disruption of Translational and Posttranslational Machinery Reshapes Growth Rates of <named-content content-type="genus-species">Bartonella birtlesii</named-content>
title_full	Partial Disruption of Translational and Posttranslational Machinery Reshapes Growth Rates of <named-content content-type="genus-species">Bartonella birtlesii</named-content>
title_fullStr	Partial Disruption of Translational and Posttranslational Machinery Reshapes Growth Rates of <named-content content-type="genus-species">Bartonella birtlesii</named-content>
title_full_unstemmed	Partial Disruption of Translational and Posttranslational Machinery Reshapes Growth Rates of <named-content content-type="genus-species">Bartonella birtlesii</named-content>
title_sort	partial disruption of translational and posttranslational machinery reshapes growth rates of <named-content content-type="genus-species">bartonella birtlesii</named-content>
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/1a09dd9faee8469eb18f2a3917ab1cb5
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Partial Disruption of Translational and Posttranslational Machinery Reshapes Growth Rates of <named-content content-type="genus-species">Bartonella birtlesii</named-content>

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