Development temperature has persistent effects on muscle growth responses in gilthead sea bream.

Initially we characterised growth responses to altered nutritional input at the transcriptional and tissue levels in the fast skeletal muscle of juvenile gilthead sea bream. Fish reared at 21-22°C (range) were fed a commercial diet at 3% body mass d(-1) (non-satiation feeding, NSF) for 4 weeks, fast...

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Autores principales:	Daniel Garcia de la serrana, Vera L A Vieira, Karl B Andree, Maria Darias, Alicia Estévez, Enric Gisbert, Ian A Johnston
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a0a3cff4ca34d84bbe9e53b5991c48c
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spelling	oai:doaj.org-article:1a0a3cff4ca34d84bbe9e53b5991c48c2021-11-18T08:04:47ZDevelopment temperature has persistent effects on muscle growth responses in gilthead sea bream.1932-620310.1371/journal.pone.0051884https://doaj.org/article/1a0a3cff4ca34d84bbe9e53b5991c48c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23284803/?tool=EBIhttps://doaj.org/toc/1932-6203Initially we characterised growth responses to altered nutritional input at the transcriptional and tissue levels in the fast skeletal muscle of juvenile gilthead sea bream. Fish reared at 21-22°C (range) were fed a commercial diet at 3% body mass d(-1) (non-satiation feeding, NSF) for 4 weeks, fasted for 4d (F) and then fed to satiation (SF) for 21d. 13 out of 34 genes investigated showed consistent patterns of regulation between nutritional states. Fasting was associated with a 20-fold increase in MAFbx, and a 5-fold increase in Six1 and WASp expression, which returned to NSF levels within 16h of SF. Refeeding to satiation was associated with a rapid (<24 h) 12 to 17-fold increase in UNC45, Hsp70 and Hsp90α transcripts coding for molecular chaperones associated with unfolded protein response pathways. The growth factors FGF6 and IGF1 increased 6.0 and 4.5-fold within 16 h and 24 h of refeeding respectively. The average growth in diameter of fast muscle fibres was checked with fasting and significant fibre hypertrophy was only observed after 13d and 21d SF. To investigate developmental plasticity in growth responses we used the same experimental protocol with fish reared at either 17.5-18.5°C (range) (LT) or 21-22°C (range) (HT) to metamorphosis and then transferred to 21-22°C. There were persistent effects of development temperature on muscle growth patterns with 20% more fibres of lower average diameter in LT than HT group of similar body size. Altering the nutritional input to the muscle to stimulate growth revealed cryptic changes in the expression of UNC45 and Hsp90α with higher transcript abundance in the LT than HT groups, whereas there were no differences in the expression of MAFbx and Six1. It was concluded that myogenesis and gene expression patterns during growth are not fixed, but can be modified by temperature during the early stages of the life cycle.Daniel Garcia de la serranaVera L A VieiraKarl B AndreeMaria DariasAlicia EstévezAlicia EstévezEnric GisbertIan A JohnstonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51884 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Garcia de la serrana Vera L A Vieira Karl B Andree Maria Darias Alicia Estévez Alicia Estévez Enric Gisbert Ian A Johnston Development temperature has persistent effects on muscle growth responses in gilthead sea bream.
description	Initially we characterised growth responses to altered nutritional input at the transcriptional and tissue levels in the fast skeletal muscle of juvenile gilthead sea bream. Fish reared at 21-22°C (range) were fed a commercial diet at 3% body mass d(-1) (non-satiation feeding, NSF) for 4 weeks, fasted for 4d (F) and then fed to satiation (SF) for 21d. 13 out of 34 genes investigated showed consistent patterns of regulation between nutritional states. Fasting was associated with a 20-fold increase in MAFbx, and a 5-fold increase in Six1 and WASp expression, which returned to NSF levels within 16h of SF. Refeeding to satiation was associated with a rapid (<24 h) 12 to 17-fold increase in UNC45, Hsp70 and Hsp90α transcripts coding for molecular chaperones associated with unfolded protein response pathways. The growth factors FGF6 and IGF1 increased 6.0 and 4.5-fold within 16 h and 24 h of refeeding respectively. The average growth in diameter of fast muscle fibres was checked with fasting and significant fibre hypertrophy was only observed after 13d and 21d SF. To investigate developmental plasticity in growth responses we used the same experimental protocol with fish reared at either 17.5-18.5°C (range) (LT) or 21-22°C (range) (HT) to metamorphosis and then transferred to 21-22°C. There were persistent effects of development temperature on muscle growth patterns with 20% more fibres of lower average diameter in LT than HT group of similar body size. Altering the nutritional input to the muscle to stimulate growth revealed cryptic changes in the expression of UNC45 and Hsp90α with higher transcript abundance in the LT than HT groups, whereas there were no differences in the expression of MAFbx and Six1. It was concluded that myogenesis and gene expression patterns during growth are not fixed, but can be modified by temperature during the early stages of the life cycle.
format	article
author	Daniel Garcia de la serrana Vera L A Vieira Karl B Andree Maria Darias Alicia Estévez Alicia Estévez Enric Gisbert Ian A Johnston
author_facet	Daniel Garcia de la serrana Vera L A Vieira Karl B Andree Maria Darias Alicia Estévez Alicia Estévez Enric Gisbert Ian A Johnston
author_sort	Daniel Garcia de la serrana
title	Development temperature has persistent effects on muscle growth responses in gilthead sea bream.
title_short	Development temperature has persistent effects on muscle growth responses in gilthead sea bream.
title_full	Development temperature has persistent effects on muscle growth responses in gilthead sea bream.
title_fullStr	Development temperature has persistent effects on muscle growth responses in gilthead sea bream.
title_full_unstemmed	Development temperature has persistent effects on muscle growth responses in gilthead sea bream.
title_sort	development temperature has persistent effects on muscle growth responses in gilthead sea bream.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1a0a3cff4ca34d84bbe9e53b5991c48c
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Development temperature has persistent effects on muscle growth responses in gilthead sea bream.

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