Segmental Additive Tissue Engineering

Abstract Segmental bone defects caused by trauma and disease represent a major clinical problem worldwide. Current treatment options are limited and often associated with poor outcomes and severe complications. Bone engineering is a promising alternative solution, but a number of technical challenge...

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Autores principales:	Martina Sladkova, Rawan Alawadhi, Rawan Jaragh Alhaddad, Asmaa Esmael, Shoug Alansari, Munerah Saad, Jenan Mulla Yousef, Lulwa Alqaoud, Giuseppe Maria de Peppo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a0af48e5e4f4ac2957fd8fbf2c31d58
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spelling	oai:doaj.org-article:1a0af48e5e4f4ac2957fd8fbf2c31d582021-12-02T15:07:57ZSegmental Additive Tissue Engineering10.1038/s41598-018-29270-42045-2322https://doaj.org/article/1a0af48e5e4f4ac2957fd8fbf2c31d582018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29270-4https://doaj.org/toc/2045-2322Abstract Segmental bone defects caused by trauma and disease represent a major clinical problem worldwide. Current treatment options are limited and often associated with poor outcomes and severe complications. Bone engineering is a promising alternative solution, but a number of technical challenges must be addressed to allow for effective and reproducible construction of segmental grafts that meet the size and geometrical requirements needed for individual patients and routine clinical applications. It is important to devise engineering strategies and standard operating procedures that make it possible to scale up the size of bone-engineered grafts, minimize process and product variability, and facilitate technology transfer and implementation. To address these issues, we have combined traditional and modular tissue engineering approaches in a strategy referred to as Segmental Additive Tissue Engineering (SATE). To demonstrate this approach, a digital reconstruction of a rabbit femoral defect was partitioned transversally to the longitudinal axis into segments (modules) with discoidal geometry and defined thickness to enable protocol standardization and effective tissue formation in vitro. Bone grafts corresponding to each segment were then engineered using biomimetic scaffolds seeded with human induced pluripotent stem cell-derived mesodermal progenitors (iPSC-MPs) and a novel perfusion bioreactor with universal design. The SATE strategy enables the effective and reproducible engineering of segmental bone grafts for personalized skeletal reconstruction, and will facilitate technology transfer and implementation of a tissue engineering approach to segmental bone defect therapy.Martina SladkovaRawan AlawadhiRawan Jaragh AlhaddadAsmaa EsmaelShoug AlansariMunerah SaadJenan Mulla YousefLulwa AlqaoudGiuseppe Maria de PeppoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martina Sladkova Rawan Alawadhi Rawan Jaragh Alhaddad Asmaa Esmael Shoug Alansari Munerah Saad Jenan Mulla Yousef Lulwa Alqaoud Giuseppe Maria de Peppo Segmental Additive Tissue Engineering
description	Abstract Segmental bone defects caused by trauma and disease represent a major clinical problem worldwide. Current treatment options are limited and often associated with poor outcomes and severe complications. Bone engineering is a promising alternative solution, but a number of technical challenges must be addressed to allow for effective and reproducible construction of segmental grafts that meet the size and geometrical requirements needed for individual patients and routine clinical applications. It is important to devise engineering strategies and standard operating procedures that make it possible to scale up the size of bone-engineered grafts, minimize process and product variability, and facilitate technology transfer and implementation. To address these issues, we have combined traditional and modular tissue engineering approaches in a strategy referred to as Segmental Additive Tissue Engineering (SATE). To demonstrate this approach, a digital reconstruction of a rabbit femoral defect was partitioned transversally to the longitudinal axis into segments (modules) with discoidal geometry and defined thickness to enable protocol standardization and effective tissue formation in vitro. Bone grafts corresponding to each segment were then engineered using biomimetic scaffolds seeded with human induced pluripotent stem cell-derived mesodermal progenitors (iPSC-MPs) and a novel perfusion bioreactor with universal design. The SATE strategy enables the effective and reproducible engineering of segmental bone grafts for personalized skeletal reconstruction, and will facilitate technology transfer and implementation of a tissue engineering approach to segmental bone defect therapy.
format	article
author	Martina Sladkova Rawan Alawadhi Rawan Jaragh Alhaddad Asmaa Esmael Shoug Alansari Munerah Saad Jenan Mulla Yousef Lulwa Alqaoud Giuseppe Maria de Peppo
author_facet	Martina Sladkova Rawan Alawadhi Rawan Jaragh Alhaddad Asmaa Esmael Shoug Alansari Munerah Saad Jenan Mulla Yousef Lulwa Alqaoud Giuseppe Maria de Peppo
author_sort	Martina Sladkova
title	Segmental Additive Tissue Engineering
title_short	Segmental Additive Tissue Engineering
title_full	Segmental Additive Tissue Engineering
title_fullStr	Segmental Additive Tissue Engineering
title_full_unstemmed	Segmental Additive Tissue Engineering
title_sort	segmental additive tissue engineering
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1a0af48e5e4f4ac2957fd8fbf2c31d58
work_keys_str_mv	AT martinasladkova segmentaladditivetissueengineering AT rawanalawadhi segmentaladditivetissueengineering AT rawanjaraghalhaddad segmentaladditivetissueengineering AT asmaaesmael segmentaladditivetissueengineering AT shougalansari segmentaladditivetissueengineering AT munerahsaad segmentaladditivetissueengineering AT jenanmullayousef segmentaladditivetissueengineering AT lulwaalqaoud segmentaladditivetissueengineering AT giuseppemariadepeppo segmentaladditivetissueengineering
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Segmental Additive Tissue Engineering

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