Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori

Transcription factors (TFs) are key proteins that modulate gene transcription and thereby lead to changes in the gene expression profile and the subsequent alteration of cellular functions. In the silk gland (SG) of silkworm Bombyx mori, an important silk-producing insect, TFs are of vital importanc...

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Autores principales:	Yan Ma, Qiwei Sun, Lihua Huang, Qin Luo, Wenhui Zeng, Yao Ou, Jingwen Ma, Hanfu Xu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a0c614aa4ce4e5faf8b4ee5fa20bef3
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spelling	oai:doaj.org-article:1a0c614aa4ce4e5faf8b4ee5fa20bef32021-11-18T08:14:33ZGenome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori1932-6203https://doaj.org/article/1a0c614aa4ce4e5faf8b4ee5fa20bef32021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584736/?tool=EBIhttps://doaj.org/toc/1932-6203Transcription factors (TFs) are key proteins that modulate gene transcription and thereby lead to changes in the gene expression profile and the subsequent alteration of cellular functions. In the silk gland (SG) of silkworm Bombyx mori, an important silk-producing insect, TFs are of vital importance in the regulation of silk protein synthesis in this organ. However, which TFs exist and express in the SG remains largely unknown. Here, we report the large-scale identification of TFs in the SG based on available full-length transcript sequences and the most recent version of silkworm genome data. In total, 348 candidate TFs were identified by strict filtration and were classified into 56 TF families. Chromosomal distribution, motif composition, and phylogenetic relationship analyses revealed the typical characteristics of these TFs. In addition, the expression patterns of 348 TFs in various tissues of B. mori, especially the SG of fourth-molt (4LM) and day-3 and day-4 fifth-instar (5L3D and 5L4D) larvae, were investigated based on public RNA-seq data and gene microarray data, followed by spatiotemporal verification of TF expression levels by quantitative real-time PCR (qRT-PCR). This report describes the first comprehensive analysis of TFs in the B. mori SG. The results can serve as a baseline for further studies of the roles of TFs in the B. mori SG.Yan MaQiwei SunLihua HuangQin LuoWenhui ZengYao OuJingwen MaHanfu XuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Ma Qiwei Sun Lihua Huang Qin Luo Wenhui Zeng Yao Ou Jingwen Ma Hanfu Xu Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori
description	Transcription factors (TFs) are key proteins that modulate gene transcription and thereby lead to changes in the gene expression profile and the subsequent alteration of cellular functions. In the silk gland (SG) of silkworm Bombyx mori, an important silk-producing insect, TFs are of vital importance in the regulation of silk protein synthesis in this organ. However, which TFs exist and express in the SG remains largely unknown. Here, we report the large-scale identification of TFs in the SG based on available full-length transcript sequences and the most recent version of silkworm genome data. In total, 348 candidate TFs were identified by strict filtration and were classified into 56 TF families. Chromosomal distribution, motif composition, and phylogenetic relationship analyses revealed the typical characteristics of these TFs. In addition, the expression patterns of 348 TFs in various tissues of B. mori, especially the SG of fourth-molt (4LM) and day-3 and day-4 fifth-instar (5L3D and 5L4D) larvae, were investigated based on public RNA-seq data and gene microarray data, followed by spatiotemporal verification of TF expression levels by quantitative real-time PCR (qRT-PCR). This report describes the first comprehensive analysis of TFs in the B. mori SG. The results can serve as a baseline for further studies of the roles of TFs in the B. mori SG.
format	article
author	Yan Ma Qiwei Sun Lihua Huang Qin Luo Wenhui Zeng Yao Ou Jingwen Ma Hanfu Xu
author_facet	Yan Ma Qiwei Sun Lihua Huang Qin Luo Wenhui Zeng Yao Ou Jingwen Ma Hanfu Xu
author_sort	Yan Ma
title	Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori
title_short	Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori
title_full	Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori
title_fullStr	Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori
title_full_unstemmed	Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori
title_sort	genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, bombyx mori
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1a0c614aa4ce4e5faf8b4ee5fa20bef3
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Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori

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