High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction

Abstract In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the status of a model organism; nonetheless, a high-resolution structure of its Photosystem II is mi...

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Autores principales:	André T. Graça, Michael Hall, Karina Persson, Wolfgang P. Schröder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a0ef970c65a45f78e8c1d3e1ffa48a0
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spelling	oai:doaj.org-article:1a0ef970c65a45f78e8c1d3e1ffa48a02021-12-02T16:23:42ZHigh-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction10.1038/s41598-021-94914-x2045-2322https://doaj.org/article/1a0ef970c65a45f78e8c1d3e1ffa48a02021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94914-xhttps://doaj.org/toc/2045-2322Abstract In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the status of a model organism; nonetheless, a high-resolution structure of its Photosystem II is missing. We present the first Cryo-EM high-resolution structure of Arabidopsis PSII supercomplex with average resolution of 2.79 Å, an important model for future PSII studies. The digitonin extracted PSII complexes demonstrate the importance of: the LHG2630-lipid-headgroup in the trimerization of the light-harvesting complex II; the stabilization of the PsbJ subunit and the CP43-loop E by DGD520-lipid; the choice of detergent for the integrity of membrane protein complexes. Furthermore, our data shows at the anticipated Mn4CaO5-site a single metal ion density as a reminiscent early stage of Photosystem II photoactivation.André T. GraçaMichael HallKarina PerssonWolfgang P. SchröderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q André T. Graça Michael Hall Karina Persson Wolfgang P. Schröder High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction
description	Abstract In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the status of a model organism; nonetheless, a high-resolution structure of its Photosystem II is missing. We present the first Cryo-EM high-resolution structure of Arabidopsis PSII supercomplex with average resolution of 2.79 Å, an important model for future PSII studies. The digitonin extracted PSII complexes demonstrate the importance of: the LHG2630-lipid-headgroup in the trimerization of the light-harvesting complex II; the stabilization of the PsbJ subunit and the CP43-loop E by DGD520-lipid; the choice of detergent for the integrity of membrane protein complexes. Furthermore, our data shows at the anticipated Mn4CaO5-site a single metal ion density as a reminiscent early stage of Photosystem II photoactivation.
format	article
author	André T. Graça Michael Hall Karina Persson Wolfgang P. Schröder
author_facet	André T. Graça Michael Hall Karina Persson Wolfgang P. Schröder
author_sort	André T. Graça
title	High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction
title_short	High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction
title_full	High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction
title_fullStr	High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction
title_full_unstemmed	High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction
title_sort	high-resolution model of arabidopsis photosystem ii reveals the structural consequences of digitonin-extraction
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1a0ef970c65a45f78e8c1d3e1ffa48a0
work_keys_str_mv	AT andretgraca highresolutionmodelofarabidopsisphotosystemiirevealsthestructuralconsequencesofdigitoninextraction AT michaelhall highresolutionmodelofarabidopsisphotosystemiirevealsthestructuralconsequencesofdigitoninextraction AT karinapersson highresolutionmodelofarabidopsisphotosystemiirevealsthestructuralconsequencesofdigitoninextraction AT wolfgangpschroder highresolutionmodelofarabidopsisphotosystemiirevealsthestructuralconsequencesofdigitoninextraction
_version_	1718384096850214912

High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction

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