A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic Bacterium Rhodothermus marinus

Rhodothermus marinus is a halophilic extreme thermophile, with potential as a model organism for studies of the structural basis of antibiotic resistance. In order to facilitate genetic studies of this organism, we have surveyed the antibiotic sensitivity spectrum of R. m...

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Autores principales:	Sophia Silvia, Samantha A. Donahue, Erin E. Killeavy, Gerwald Jogl, Steven T. Gregory
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Rhodothermus marinus</i> antibiotic-resistance mutation ribosome RNA polymerase halophile thermophile Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/1a18f87c42764ee38afae6120ef9ca77
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spelling	oai:doaj.org-article:1a18f87c42764ee38afae6120ef9ca772021-11-25T16:24:26ZA Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic Bacterium <i>Rhodothermus marinus</i>10.3390/antibiotics101113842079-6382https://doaj.org/article/1a18f87c42764ee38afae6120ef9ca772021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1384https://doaj.org/toc/2079-6382<i>Rhodothermus marinus</i> is a halophilic extreme thermophile, with potential as a model organism for studies of the structural basis of antibiotic resistance. In order to facilitate genetic studies of this organism, we have surveyed the antibiotic sensitivity spectrum of <i>R. marinus</i> and identified spontaneous antibiotic-resistant mutants. <i>R. marinus</i> is naturally insensitive to aminoglycosides, aminocylitols and tuberactinomycins that target the 30S ribosomal subunit, but is sensitive to all 50S ribosomal subunit-targeting antibiotics examined, including macrolides, lincosamides, streptogramin B, chloramphenicol, and thiostrepton. It is also sensitive to kirromycin and fusidic acid, which target protein synthesis factors. It is sensitive to rifampicin (RNA polymerase inhibitor) and to the fluoroquinolones ofloxacin and ciprofloxacin (DNA gyrase inhibitors), but insensitive to nalidixic acid. Drug-resistant mutants were identified using rifampicin, thiostrepton, erythromycin, spiramycin, tylosin, lincomycin, and chloramphenicol. The majority of these were found to have mutations that are similar or identical to those previously found in other species, while several novel mutations were identified. This study provides potential selectable markers for genetic manipulations and demonstrates the feasibility of using <i>R. marinus</i> as a model system for studies of ribosome and RNA polymerase structure, function, and evolution.Sophia SilviaSamantha A. DonahueErin E. KilleavyGerwald JoglSteven T. GregoryMDPI AGarticle<i>Rhodothermus marinus</i>antibiotic-resistance mutationribosomeRNA polymerasehalophilethermophileTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1384, p 1384 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Rhodothermus marinus</i> antibiotic-resistance mutation ribosome RNA polymerase halophile thermophile Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Rhodothermus marinus</i> antibiotic-resistance mutation ribosome RNA polymerase halophile thermophile Therapeutics. Pharmacology RM1-950 Sophia Silvia Samantha A. Donahue Erin E. Killeavy Gerwald Jogl Steven T. Gregory A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic Bacterium <i>Rhodothermus marinus</i>
description	<i>Rhodothermus marinus</i> is a halophilic extreme thermophile, with potential as a model organism for studies of the structural basis of antibiotic resistance. In order to facilitate genetic studies of this organism, we have surveyed the antibiotic sensitivity spectrum of <i>R. marinus</i> and identified spontaneous antibiotic-resistant mutants. <i>R. marinus</i> is naturally insensitive to aminoglycosides, aminocylitols and tuberactinomycins that target the 30S ribosomal subunit, but is sensitive to all 50S ribosomal subunit-targeting antibiotics examined, including macrolides, lincosamides, streptogramin B, chloramphenicol, and thiostrepton. It is also sensitive to kirromycin and fusidic acid, which target protein synthesis factors. It is sensitive to rifampicin (RNA polymerase inhibitor) and to the fluoroquinolones ofloxacin and ciprofloxacin (DNA gyrase inhibitors), but insensitive to nalidixic acid. Drug-resistant mutants were identified using rifampicin, thiostrepton, erythromycin, spiramycin, tylosin, lincomycin, and chloramphenicol. The majority of these were found to have mutations that are similar or identical to those previously found in other species, while several novel mutations were identified. This study provides potential selectable markers for genetic manipulations and demonstrates the feasibility of using <i>R. marinus</i> as a model system for studies of ribosome and RNA polymerase structure, function, and evolution.
format	article
author	Sophia Silvia Samantha A. Donahue Erin E. Killeavy Gerwald Jogl Steven T. Gregory
author_facet	Sophia Silvia Samantha A. Donahue Erin E. Killeavy Gerwald Jogl Steven T. Gregory
author_sort	Sophia Silvia
title	A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic Bacterium <i>Rhodothermus marinus</i>
title_short	A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic Bacterium <i>Rhodothermus marinus</i>
title_full	A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic Bacterium <i>Rhodothermus marinus</i>
title_fullStr	A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic Bacterium <i>Rhodothermus marinus</i>
title_full_unstemmed	A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic Bacterium <i>Rhodothermus marinus</i>
title_sort	survey of spontaneous antibiotic-resistant mutants of the halophilic, thermophilic bacterium <i>rhodothermus marinus</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1a18f87c42764ee38afae6120ef9ca77
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A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic Bacterium <i>Rhodothermus marinus</i>

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