The Hitchhiker’s Guide to Untargeted Lipidomics Analysis: Practical Guidelines

Lipidomics is a newly emerged discipline involving the identification and quantification of thousands of lipids. As a part of the omics field, lipidomics has shown rapid growth both in the number of studies and in the size of lipidome datasets, thus, requiring specific and efficient data analysis ap...

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Autores principales:	Dmitrii Smirnov, Pavel Mazin, Maria Osetrova, Elena Stekolshchikova, Ekaterina Khrameeva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lipidome LC-MS bioinformatics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1a1eb8bce0e343aaa958b17fc2032aa1
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id	oai:doaj.org-article:1a1eb8bce0e343aaa958b17fc2032aa1
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spelling	oai:doaj.org-article:1a1eb8bce0e343aaa958b17fc2032aa12021-11-25T18:20:22ZThe Hitchhiker’s Guide to Untargeted Lipidomics Analysis: Practical Guidelines10.3390/metabo111107132218-1989https://doaj.org/article/1a1eb8bce0e343aaa958b17fc2032aa12021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/713https://doaj.org/toc/2218-1989Lipidomics is a newly emerged discipline involving the identification and quantification of thousands of lipids. As a part of the omics field, lipidomics has shown rapid growth both in the number of studies and in the size of lipidome datasets, thus, requiring specific and efficient data analysis approaches. This paper aims to provide guidelines for analyzing and interpreting lipidome data obtained using untargeted methods that rely on liquid chromatography coupled with mass spectrometry (LC-MS) to detect and measure the intensities of lipid compounds. We present a state-of-the-art untargeted LC-MS workflow for lipidomics, from study design to annotation of lipid features, focusing on practical, rather than theoretical, approaches for data analysis, and we outline possible applications of untargeted lipidomics for biological studies. We provide a detailed R notebook designed specifically for untargeted lipidome LC-MS data analysis, which is based on <i>xcms</i> software.Dmitrii SmirnovPavel MazinMaria OsetrovaElena StekolshchikovaEkaterina KhrameevaMDPI AGarticlelipidomeLC-MSbioinformaticsMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 713, p 713 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipidome LC-MS bioinformatics Microbiology QR1-502
spellingShingle	lipidome LC-MS bioinformatics Microbiology QR1-502 Dmitrii Smirnov Pavel Mazin Maria Osetrova Elena Stekolshchikova Ekaterina Khrameeva The Hitchhiker’s Guide to Untargeted Lipidomics Analysis: Practical Guidelines
description	Lipidomics is a newly emerged discipline involving the identification and quantification of thousands of lipids. As a part of the omics field, lipidomics has shown rapid growth both in the number of studies and in the size of lipidome datasets, thus, requiring specific and efficient data analysis approaches. This paper aims to provide guidelines for analyzing and interpreting lipidome data obtained using untargeted methods that rely on liquid chromatography coupled with mass spectrometry (LC-MS) to detect and measure the intensities of lipid compounds. We present a state-of-the-art untargeted LC-MS workflow for lipidomics, from study design to annotation of lipid features, focusing on practical, rather than theoretical, approaches for data analysis, and we outline possible applications of untargeted lipidomics for biological studies. We provide a detailed R notebook designed specifically for untargeted lipidome LC-MS data analysis, which is based on <i>xcms</i> software.
format	article
author	Dmitrii Smirnov Pavel Mazin Maria Osetrova Elena Stekolshchikova Ekaterina Khrameeva
author_facet	Dmitrii Smirnov Pavel Mazin Maria Osetrova Elena Stekolshchikova Ekaterina Khrameeva
author_sort	Dmitrii Smirnov
title	The Hitchhiker’s Guide to Untargeted Lipidomics Analysis: Practical Guidelines
title_short	The Hitchhiker’s Guide to Untargeted Lipidomics Analysis: Practical Guidelines
title_full	The Hitchhiker’s Guide to Untargeted Lipidomics Analysis: Practical Guidelines
title_fullStr	The Hitchhiker’s Guide to Untargeted Lipidomics Analysis: Practical Guidelines
title_full_unstemmed	The Hitchhiker’s Guide to Untargeted Lipidomics Analysis: Practical Guidelines
title_sort	hitchhiker’s guide to untargeted lipidomics analysis: practical guidelines
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1a1eb8bce0e343aaa958b17fc2032aa1
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The Hitchhiker’s Guide to Untargeted Lipidomics Analysis: Practical Guidelines

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