Transcriptome analysis of human dermal fibroblasts following red light phototherapy

Abstract Fibrosis occurs when collagen deposition and fibroblast proliferation replace healthy tissue. Red light (RL) may improve skin fibrosis via photobiomodulation, the process by which photosensitive chromophores in cells absorb visible or near-infrared light and undergo photophysical reactions....

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Autores principales:	Evan Austin, Eugene Koo, Alexander Merleev, Denis Torre, Alina Marusina, Guillaume Luxardi, Andrew Mamalis, Roslyn Rivkah Isseroff, Avi Ma’ayan, Emanual Maverakis, Jared Jagdeo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a227c143dba4b9495dc0bf4c116257c
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spelling	oai:doaj.org-article:1a227c143dba4b9495dc0bf4c116257c2021-12-02T18:18:06ZTranscriptome analysis of human dermal fibroblasts following red light phototherapy10.1038/s41598-021-86623-22045-2322https://doaj.org/article/1a227c143dba4b9495dc0bf4c116257c2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86623-2https://doaj.org/toc/2045-2322Abstract Fibrosis occurs when collagen deposition and fibroblast proliferation replace healthy tissue. Red light (RL) may improve skin fibrosis via photobiomodulation, the process by which photosensitive chromophores in cells absorb visible or near-infrared light and undergo photophysical reactions. Our previous research demonstrated that high fluence RL reduces fibroblast proliferation, collagen deposition, and migration. Despite the identification of several cellular mechanisms underpinning RL phototherapy, little is known about the transcriptional changes that lead to anti-fibrotic cellular responses. Herein, RNA sequencing was performed on human dermal fibroblasts treated with RL phototherapy. Pathway enrichment and transcription factor analysis revealed regulation of extracellular matrices, proliferation, and cellular responses to oxygen-containing compounds following RL phototherapy. Specifically, RL phototherapy increased the expression of MMP1, which codes for matrix metalloproteinase-1 (MMP-1) and is responsible for remodeling extracellular collagen. Differential regulation of MMP1 was confirmed with RT-qPCR and ELISA. Additionally, RL upregulated PRSS35, which has not been previously associated with skin activity, but has known anti-fibrotic functions. Our results suggest that RL may benefit patients by altering fibrotic gene expression.Evan AustinEugene KooAlexander MerleevDenis TorreAlina MarusinaGuillaume LuxardiAndrew MamalisRoslyn Rivkah IsseroffAvi Ma’ayanEmanual MaverakisJared JagdeoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evan Austin Eugene Koo Alexander Merleev Denis Torre Alina Marusina Guillaume Luxardi Andrew Mamalis Roslyn Rivkah Isseroff Avi Ma’ayan Emanual Maverakis Jared Jagdeo Transcriptome analysis of human dermal fibroblasts following red light phototherapy
description	Abstract Fibrosis occurs when collagen deposition and fibroblast proliferation replace healthy tissue. Red light (RL) may improve skin fibrosis via photobiomodulation, the process by which photosensitive chromophores in cells absorb visible or near-infrared light and undergo photophysical reactions. Our previous research demonstrated that high fluence RL reduces fibroblast proliferation, collagen deposition, and migration. Despite the identification of several cellular mechanisms underpinning RL phototherapy, little is known about the transcriptional changes that lead to anti-fibrotic cellular responses. Herein, RNA sequencing was performed on human dermal fibroblasts treated with RL phototherapy. Pathway enrichment and transcription factor analysis revealed regulation of extracellular matrices, proliferation, and cellular responses to oxygen-containing compounds following RL phototherapy. Specifically, RL phototherapy increased the expression of MMP1, which codes for matrix metalloproteinase-1 (MMP-1) and is responsible for remodeling extracellular collagen. Differential regulation of MMP1 was confirmed with RT-qPCR and ELISA. Additionally, RL upregulated PRSS35, which has not been previously associated with skin activity, but has known anti-fibrotic functions. Our results suggest that RL may benefit patients by altering fibrotic gene expression.
format	article
author	Evan Austin Eugene Koo Alexander Merleev Denis Torre Alina Marusina Guillaume Luxardi Andrew Mamalis Roslyn Rivkah Isseroff Avi Ma’ayan Emanual Maverakis Jared Jagdeo
author_facet	Evan Austin Eugene Koo Alexander Merleev Denis Torre Alina Marusina Guillaume Luxardi Andrew Mamalis Roslyn Rivkah Isseroff Avi Ma’ayan Emanual Maverakis Jared Jagdeo
author_sort	Evan Austin
title	Transcriptome analysis of human dermal fibroblasts following red light phototherapy
title_short	Transcriptome analysis of human dermal fibroblasts following red light phototherapy
title_full	Transcriptome analysis of human dermal fibroblasts following red light phototherapy
title_fullStr	Transcriptome analysis of human dermal fibroblasts following red light phototherapy
title_full_unstemmed	Transcriptome analysis of human dermal fibroblasts following red light phototherapy
title_sort	transcriptome analysis of human dermal fibroblasts following red light phototherapy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1a227c143dba4b9495dc0bf4c116257c
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Transcriptome analysis of human dermal fibroblasts following red light phototherapy

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