A proximity labeling protocol to probe proximity interactions in C. elegans

Summary: Enzyme-catalyzed proximity labeling (PL) has emerged as a critical approach for identifying protein-protein proximity interactions in cells; however, PL techniques were not historically practical in living multicellular organisms due to technical limitations. Here, we present a protocol for...

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Autores principales:	Ariana D. Sanchez, Jessica L. Feldman
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Developmental biology Microscopy Model Organisms Molecular Biology Molecular/Chemical Probes Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/1a2365f045a3463d9115801484abdd9f
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spelling	oai:doaj.org-article:1a2365f045a3463d9115801484abdd9f2021-12-04T04:35:59ZA proximity labeling protocol to probe proximity interactions in C. elegans2666-166710.1016/j.xpro.2021.100986https://doaj.org/article/1a2365f045a3463d9115801484abdd9f2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006924https://doaj.org/toc/2666-1667Summary: Enzyme-catalyzed proximity labeling (PL) has emerged as a critical approach for identifying protein-protein proximity interactions in cells; however, PL techniques were not historically practical in living multicellular organisms due to technical limitations. Here, we present a protocol for applying PL to living C. elegans using the biotin ligase mutant enzyme TurboID. We demonstrated PL in a tissue-specific and region-specific manner by focusing on non-centrosomal MTOCs (ncMTOCs) of intestinal cells. This protocol is useful for targeted in vivo protein network profiling.For complete details on the use and execution of this protocol, please refer to Sanchez et al. (2021).Ariana D. SanchezJessica L. FeldmanElsevierarticleCell BiologyDevelopmental biologyMicroscopyModel OrganismsMolecular BiologyMolecular/Chemical ProbesScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100986- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Developmental biology Microscopy Model Organisms Molecular Biology Molecular/Chemical Probes Science (General) Q1-390
spellingShingle	Cell Biology Developmental biology Microscopy Model Organisms Molecular Biology Molecular/Chemical Probes Science (General) Q1-390 Ariana D. Sanchez Jessica L. Feldman A proximity labeling protocol to probe proximity interactions in C. elegans
description	Summary: Enzyme-catalyzed proximity labeling (PL) has emerged as a critical approach for identifying protein-protein proximity interactions in cells; however, PL techniques were not historically practical in living multicellular organisms due to technical limitations. Here, we present a protocol for applying PL to living C. elegans using the biotin ligase mutant enzyme TurboID. We demonstrated PL in a tissue-specific and region-specific manner by focusing on non-centrosomal MTOCs (ncMTOCs) of intestinal cells. This protocol is useful for targeted in vivo protein network profiling.For complete details on the use and execution of this protocol, please refer to Sanchez et al. (2021).
format	article
author	Ariana D. Sanchez Jessica L. Feldman
author_facet	Ariana D. Sanchez Jessica L. Feldman
author_sort	Ariana D. Sanchez
title	A proximity labeling protocol to probe proximity interactions in C. elegans
title_short	A proximity labeling protocol to probe proximity interactions in C. elegans
title_full	A proximity labeling protocol to probe proximity interactions in C. elegans
title_fullStr	A proximity labeling protocol to probe proximity interactions in C. elegans
title_full_unstemmed	A proximity labeling protocol to probe proximity interactions in C. elegans
title_sort	proximity labeling protocol to probe proximity interactions in c. elegans
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/1a2365f045a3463d9115801484abdd9f
work_keys_str_mv	AT arianadsanchez aproximitylabelingprotocoltoprobeproximityinteractionsincelegans AT jessicalfeldman aproximitylabelingprotocoltoprobeproximityinteractionsincelegans AT arianadsanchez proximitylabelingprotocoltoprobeproximityinteractionsincelegans AT jessicalfeldman proximitylabelingprotocoltoprobeproximityinteractionsincelegans
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A proximity labeling protocol to probe proximity interactions in C. elegans

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