Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (<i>Apis mellifera</i>) Digestive Tract

Pesticides are increasing honeybee (<i>Apis mellifera</i>) death rates globally. Clothianidin neonicotinoid appears to impair the microbe–immunity axis. We conducted cage experiments on newly emerged bees that were 4–6 days old and used a 16S rRNA metataxonomic approach to measure the im...

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Autores principales:	Sarah El Khoury, Jeff Gauthier, Sidki Bouslama, Bachar Cheaib, Pierre Giovenazzo, Nicolas Derome
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	honeybee clothianidin microbiota dysbiosis network analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1a2fe3ef49504dbaaa2fe23639aacb34
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spelling	oai:doaj.org-article:1a2fe3ef49504dbaaa2fe23639aacb342021-11-25T18:24:50ZDietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (<i>Apis mellifera</i>) Digestive Tract10.3390/microorganisms91122832076-2607https://doaj.org/article/1a2fe3ef49504dbaaa2fe23639aacb342021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2283https://doaj.org/toc/2076-2607Pesticides are increasing honeybee (<i>Apis mellifera</i>) death rates globally. Clothianidin neonicotinoid appears to impair the microbe–immunity axis. We conducted cage experiments on newly emerged bees that were 4–6 days old and used a 16S rRNA metataxonomic approach to measure the impact of three sublethal clothianidin concentrations (0.1, 1 and 10 ppb) on survival, sucrose syrup consumption and gut microbiota community structure. Exposure to clothianidin significantly increased mortality in the three concentrations compared to controls. Interestingly, the lowest clothianidin concentration was associated with the highest mortality, and the medium concentration with the highest food intake. Exposure to clothianidin induced significant variation in the taxonomic distribution of gut microbiota activity. Co-abundance network analysis revealed local dysbiosis signatures specific to each gut section (midgut, ileum and rectum) were driven by specific taxa. Our findings confirm that exposure to clothianidin triggers a reshuffling of beneficial strains and/or potentially pathogenic taxa within the gut, suggesting a honeybee’s symbiotic defense systems’ disruption, such as resistance to microbial colonization. This study highlights the role of weak transcriptional activity taxa in maintaining a stable honeybee gut microbiota. Finally, the early detection of gut dysbiosis in honeybees is a promising biomarker in hive management for assessing the impact exposure to sublethal xenobiotics.Sarah El KhouryJeff GauthierSidki BouslamaBachar CheaibPierre GiovenazzoNicolas DeromeMDPI AGarticlehoneybeeclothianidinmicrobiotadysbiosisnetwork analysisBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2283, p 2283 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	honeybee clothianidin microbiota dysbiosis network analysis Biology (General) QH301-705.5
spellingShingle	honeybee clothianidin microbiota dysbiosis network analysis Biology (General) QH301-705.5 Sarah El Khoury Jeff Gauthier Sidki Bouslama Bachar Cheaib Pierre Giovenazzo Nicolas Derome Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (<i>Apis mellifera</i>) Digestive Tract
description	Pesticides are increasing honeybee (<i>Apis mellifera</i>) death rates globally. Clothianidin neonicotinoid appears to impair the microbe–immunity axis. We conducted cage experiments on newly emerged bees that were 4–6 days old and used a 16S rRNA metataxonomic approach to measure the impact of three sublethal clothianidin concentrations (0.1, 1 and 10 ppb) on survival, sucrose syrup consumption and gut microbiota community structure. Exposure to clothianidin significantly increased mortality in the three concentrations compared to controls. Interestingly, the lowest clothianidin concentration was associated with the highest mortality, and the medium concentration with the highest food intake. Exposure to clothianidin induced significant variation in the taxonomic distribution of gut microbiota activity. Co-abundance network analysis revealed local dysbiosis signatures specific to each gut section (midgut, ileum and rectum) were driven by specific taxa. Our findings confirm that exposure to clothianidin triggers a reshuffling of beneficial strains and/or potentially pathogenic taxa within the gut, suggesting a honeybee’s symbiotic defense systems’ disruption, such as resistance to microbial colonization. This study highlights the role of weak transcriptional activity taxa in maintaining a stable honeybee gut microbiota. Finally, the early detection of gut dysbiosis in honeybees is a promising biomarker in hive management for assessing the impact exposure to sublethal xenobiotics.
format	article
author	Sarah El Khoury Jeff Gauthier Sidki Bouslama Bachar Cheaib Pierre Giovenazzo Nicolas Derome
author_facet	Sarah El Khoury Jeff Gauthier Sidki Bouslama Bachar Cheaib Pierre Giovenazzo Nicolas Derome
author_sort	Sarah El Khoury
title	Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (<i>Apis mellifera</i>) Digestive Tract
title_short	Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (<i>Apis mellifera</i>) Digestive Tract
title_full	Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (<i>Apis mellifera</i>) Digestive Tract
title_fullStr	Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (<i>Apis mellifera</i>) Digestive Tract
title_full_unstemmed	Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (<i>Apis mellifera</i>) Digestive Tract
title_sort	dietary contamination with a neonicotinoid (clothianidin) gradient triggers specific dysbiosis signatures of microbiota activity along the honeybee (<i>apis mellifera</i>) digestive tract
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1a2fe3ef49504dbaaa2fe23639aacb34
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Dietary Contamination with a Neonicotinoid (Clothianidin) Gradient Triggers Specific Dysbiosis Signatures of Microbiota Activity along the Honeybee (<i>Apis mellifera</i>) Digestive Tract

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