Coordinated activation of a cluster of MMP genes in response to UVB radiation

Abstract Ultraviolet (UV) B radiation is a dangerous environmental stressor, which can lead to photoaging, inflammation, immune suppression and tumour formation. A recent report has shown the transcriptional activation of several skin-specific genes including matrix metalloproteases (MMPs) in respon...

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Autores principales:	Zsuzsanna Ujfaludi, Agota Tuzesi, Hajnalka Majoros, Balint Rothler, Tibor Pankotai, Imre M. Boros
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a3685708ff34909965e8ff359b8fb21
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spelling	oai:doaj.org-article:1a3685708ff34909965e8ff359b8fb212021-12-02T15:08:34ZCoordinated activation of a cluster of MMP genes in response to UVB radiation10.1038/s41598-018-20999-62045-2322https://doaj.org/article/1a3685708ff34909965e8ff359b8fb212018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20999-6https://doaj.org/toc/2045-2322Abstract Ultraviolet (UV) B radiation is a dangerous environmental stressor, which can lead to photoaging, inflammation, immune suppression and tumour formation. A recent report has shown the transcriptional activation of several skin-specific genes including matrix metalloproteases (MMPs) in response to UV irradiation. Here, we use a novel human keratinocyte model, HKerE6SFM, to demonstrate that UVB activates the transcription of most members of the 11q22.3 MMP gene cluster including MMP13, MMP12, MMP3, MMP1 and MMP10. Curiously, the expression of the well-characterized UVB-inducible MMP9, which is located outside of the cluster, remains unchanged. In accordance with the increased expression of the MMP gene cluster upon UVB irradiation, RNA polymerase II showed increased occupancy at their promoters following UVB irradiation. The results also demonstrate increased acetylated histone H3K9 levels at the promoters of the MMP13, MMP12, MMP3, MMP1 and MMP10 genes. These findings suggest a coordinated transcriptional activation of genes in the MMP cluster at 11q22.3 and that acetylation of histone H3 at lysine 9 has an important role in the UVB-dependent enhancement of transcription of MMP genes in this region.Zsuzsanna UjfaludiAgota TuzesiHajnalka MajorosBalint RothlerTibor PankotaiImre M. BorosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zsuzsanna Ujfaludi Agota Tuzesi Hajnalka Majoros Balint Rothler Tibor Pankotai Imre M. Boros Coordinated activation of a cluster of MMP genes in response to UVB radiation
description	Abstract Ultraviolet (UV) B radiation is a dangerous environmental stressor, which can lead to photoaging, inflammation, immune suppression and tumour formation. A recent report has shown the transcriptional activation of several skin-specific genes including matrix metalloproteases (MMPs) in response to UV irradiation. Here, we use a novel human keratinocyte model, HKerE6SFM, to demonstrate that UVB activates the transcription of most members of the 11q22.3 MMP gene cluster including MMP13, MMP12, MMP3, MMP1 and MMP10. Curiously, the expression of the well-characterized UVB-inducible MMP9, which is located outside of the cluster, remains unchanged. In accordance with the increased expression of the MMP gene cluster upon UVB irradiation, RNA polymerase II showed increased occupancy at their promoters following UVB irradiation. The results also demonstrate increased acetylated histone H3K9 levels at the promoters of the MMP13, MMP12, MMP3, MMP1 and MMP10 genes. These findings suggest a coordinated transcriptional activation of genes in the MMP cluster at 11q22.3 and that acetylation of histone H3 at lysine 9 has an important role in the UVB-dependent enhancement of transcription of MMP genes in this region.
format	article
author	Zsuzsanna Ujfaludi Agota Tuzesi Hajnalka Majoros Balint Rothler Tibor Pankotai Imre M. Boros
author_facet	Zsuzsanna Ujfaludi Agota Tuzesi Hajnalka Majoros Balint Rothler Tibor Pankotai Imre M. Boros
author_sort	Zsuzsanna Ujfaludi
title	Coordinated activation of a cluster of MMP genes in response to UVB radiation
title_short	Coordinated activation of a cluster of MMP genes in response to UVB radiation
title_full	Coordinated activation of a cluster of MMP genes in response to UVB radiation
title_fullStr	Coordinated activation of a cluster of MMP genes in response to UVB radiation
title_full_unstemmed	Coordinated activation of a cluster of MMP genes in response to UVB radiation
title_sort	coordinated activation of a cluster of mmp genes in response to uvb radiation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1a3685708ff34909965e8ff359b8fb21
work_keys_str_mv	AT zsuzsannaujfaludi coordinatedactivationofaclusterofmmpgenesinresponsetouvbradiation AT agotatuzesi coordinatedactivationofaclusterofmmpgenesinresponsetouvbradiation AT hajnalkamajoros coordinatedactivationofaclusterofmmpgenesinresponsetouvbradiation AT balintrothler coordinatedactivationofaclusterofmmpgenesinresponsetouvbradiation AT tiborpankotai coordinatedactivationofaclusterofmmpgenesinresponsetouvbradiation AT imremboros coordinatedactivationofaclusterofmmpgenesinresponsetouvbradiation
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Coordinated activation of a cluster of MMP genes in response to UVB radiation

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