Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation

Abstract Exiguobacterium sp. AO-11 was immobilized on bio-cord at 109 CFU g−1 carrier for the removal of crude oil from marine environments. To prepare a ready-to-use bioremediation product, the shelf life of the immobilized cells was calculated. Approximately 90% of 0.25% (v/v) crude oil removal wa...

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Autores principales:	Chatsuda Sakdapetsiri, Nitchakarn Kaokhum, Onruthai Pinyakong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a3c8c504bf8434abb8e25af2d18eeff
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spelling	oai:doaj.org-article:1a3c8c504bf8434abb8e25af2d18eeff2021-12-02T16:06:07ZBiodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation10.1038/s41598-021-92122-12045-2322https://doaj.org/article/1a3c8c504bf8434abb8e25af2d18eeff2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92122-1https://doaj.org/toc/2045-2322Abstract Exiguobacterium sp. AO-11 was immobilized on bio-cord at 109 CFU g−1 carrier for the removal of crude oil from marine environments. To prepare a ready-to-use bioremediation product, the shelf life of the immobilized cells was calculated. Approximately 90% of 0.25% (v/v) crude oil removal was achieved within 9 days when the starved state of immobilized cells was used. The oil removal activity of the immobilized cells was maintained in the presence of oil dispersant (89%) and at pH values of 7–9. Meanwhile, pH, oil concentration and salinity affected the oil removal efficacy. The immobilized cells could be reused for at least 5 cycles. The Arrhenius equation describing the relationship between the rate of reaction and temperature was validated as a useful model of the kinetics of retention of activity by an immobilized biocatalyst. It was estimated that the immobilized cells could be stored in a non-vacuum bag containing phosphate buffer (pH 7.0) at 30 °C for 39 days to retain the cells at 107 CFU g−1 carrier and more than 50% degradation activity. These results indicated the potential of using bio-cord-immobilized crude oil-degrading Exiguobacterium sp. AO-11 as a bioremediation product in a marine environment.Chatsuda SakdapetsiriNitchakarn KaokhumOnruthai PinyakongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chatsuda Sakdapetsiri Nitchakarn Kaokhum Onruthai Pinyakong Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation
description	Abstract Exiguobacterium sp. AO-11 was immobilized on bio-cord at 109 CFU g−1 carrier for the removal of crude oil from marine environments. To prepare a ready-to-use bioremediation product, the shelf life of the immobilized cells was calculated. Approximately 90% of 0.25% (v/v) crude oil removal was achieved within 9 days when the starved state of immobilized cells was used. The oil removal activity of the immobilized cells was maintained in the presence of oil dispersant (89%) and at pH values of 7–9. Meanwhile, pH, oil concentration and salinity affected the oil removal efficacy. The immobilized cells could be reused for at least 5 cycles. The Arrhenius equation describing the relationship between the rate of reaction and temperature was validated as a useful model of the kinetics of retention of activity by an immobilized biocatalyst. It was estimated that the immobilized cells could be stored in a non-vacuum bag containing phosphate buffer (pH 7.0) at 30 °C for 39 days to retain the cells at 107 CFU g−1 carrier and more than 50% degradation activity. These results indicated the potential of using bio-cord-immobilized crude oil-degrading Exiguobacterium sp. AO-11 as a bioremediation product in a marine environment.
format	article
author	Chatsuda Sakdapetsiri Nitchakarn Kaokhum Onruthai Pinyakong
author_facet	Chatsuda Sakdapetsiri Nitchakarn Kaokhum Onruthai Pinyakong
author_sort	Chatsuda Sakdapetsiri
title	Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation
title_short	Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation
title_full	Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation
title_fullStr	Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation
title_full_unstemmed	Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation
title_sort	biodegradation of crude oil by immobilized exiguobacterium sp. ao-11 and shelf life evaluation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1a3c8c504bf8434abb8e25af2d18eeff
work_keys_str_mv	AT chatsudasakdapetsiri biodegradationofcrudeoilbyimmobilizedexiguobacteriumspao11andshelflifeevaluation AT nitchakarnkaokhum biodegradationofcrudeoilbyimmobilizedexiguobacteriumspao11andshelflifeevaluation AT onruthaipinyakong biodegradationofcrudeoilbyimmobilizedexiguobacteriumspao11andshelflifeevaluation
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Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation

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