Multiplex profiling of cellular invasion in 3D cell culture models.

To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces. Here, we describe a 96-well microplate-based, high-content, three-dimensional cell culture assay capable of assessi...

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Autores principales:	Gerald Burgstaller, Bettina Oehrle, Ina Koch, Michael Lindner, Oliver Eickelberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a4301e54d784a8c842c7f3cdbda79fa
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spelling	oai:doaj.org-article:1a4301e54d784a8c842c7f3cdbda79fa2021-11-18T07:46:12ZMultiplex profiling of cellular invasion in 3D cell culture models.1932-620310.1371/journal.pone.0063121https://doaj.org/article/1a4301e54d784a8c842c7f3cdbda79fa2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23671660/?tool=EBIhttps://doaj.org/toc/1932-6203To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces. Here, we describe a 96-well microplate-based, high-content, three-dimensional cell culture assay capable of assessing invasion dynamics and molecular signatures thereof. On applying our invasion assay, we were able to demonstrate significant effects on the invasion capacity of fibroblast cell lines, as well as primary lung fibroblasts. Administration of epidermal growth factor resulted in a substantial increase of cellular invasion, thus making this technique suitable for high-throughput pharmacological screening of novel compounds regulating invasive and migratory pathways of primary cells. Our assay also correlates cellular invasiveness to molecular events. Thus, we argue of having developed a powerful and versatile toolbox for an extensive profiling of invasive cells in a 96-well format. This will have a major impact on research in disease areas like fibrosis, metastatic cancers, or chronic inflammatory states.Gerald BurgstallerBettina OehrleIna KochMichael LindnerOliver EickelbergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63121 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gerald Burgstaller Bettina Oehrle Ina Koch Michael Lindner Oliver Eickelberg Multiplex profiling of cellular invasion in 3D cell culture models.
description	To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces. Here, we describe a 96-well microplate-based, high-content, three-dimensional cell culture assay capable of assessing invasion dynamics and molecular signatures thereof. On applying our invasion assay, we were able to demonstrate significant effects on the invasion capacity of fibroblast cell lines, as well as primary lung fibroblasts. Administration of epidermal growth factor resulted in a substantial increase of cellular invasion, thus making this technique suitable for high-throughput pharmacological screening of novel compounds regulating invasive and migratory pathways of primary cells. Our assay also correlates cellular invasiveness to molecular events. Thus, we argue of having developed a powerful and versatile toolbox for an extensive profiling of invasive cells in a 96-well format. This will have a major impact on research in disease areas like fibrosis, metastatic cancers, or chronic inflammatory states.
format	article
author	Gerald Burgstaller Bettina Oehrle Ina Koch Michael Lindner Oliver Eickelberg
author_facet	Gerald Burgstaller Bettina Oehrle Ina Koch Michael Lindner Oliver Eickelberg
author_sort	Gerald Burgstaller
title	Multiplex profiling of cellular invasion in 3D cell culture models.
title_short	Multiplex profiling of cellular invasion in 3D cell culture models.
title_full	Multiplex profiling of cellular invasion in 3D cell culture models.
title_fullStr	Multiplex profiling of cellular invasion in 3D cell culture models.
title_full_unstemmed	Multiplex profiling of cellular invasion in 3D cell culture models.
title_sort	multiplex profiling of cellular invasion in 3d cell culture models.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1a4301e54d784a8c842c7f3cdbda79fa
work_keys_str_mv	AT geraldburgstaller multiplexprofilingofcellularinvasionin3dcellculturemodels AT bettinaoehrle multiplexprofilingofcellularinvasionin3dcellculturemodels AT inakoch multiplexprofilingofcellularinvasionin3dcellculturemodels AT michaellindner multiplexprofilingofcellularinvasionin3dcellculturemodels AT olivereickelberg multiplexprofilingofcellularinvasionin3dcellculturemodels
_version_	1718422957509836800

Multiplex profiling of cellular invasion in 3D cell culture models.

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