Mutational disruption of the ABCC2 gene in fall armyworm, Spodoptera frugiperda, confers resistance to the Cry1Fa and Cry1A.105 insecticidal proteins

Abstract The use of Bt proteins in crops has revolutionized insect pest management by offering effective season-long control. However, field-evolved resistance to Bt proteins threatens their utility and durability. A recent example is field-evolved resistance to Cry1Fa and Cry1A.105 in fall armyworm...

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Autores principales:	Lex Flagel, Young Wha Lee, Humphrey Wanjugi, Shilpa Swarup, Alana Brown, Jinling Wang, Edward Kraft, John Greenplate, Jeni Simmons, Nancy Adams, Yanfei Wang, Samuel Martinelli, Jeffrey A. Haas, Anilkumar Gowda, Graham Head
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a4671474271486f96a8ae1bd2ffb2cf
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spelling	oai:doaj.org-article:1a4671474271486f96a8ae1bd2ffb2cf2021-12-02T11:40:35ZMutational disruption of the ABCC2 gene in fall armyworm, Spodoptera frugiperda, confers resistance to the Cry1Fa and Cry1A.105 insecticidal proteins10.1038/s41598-018-25491-92045-2322https://doaj.org/article/1a4671474271486f96a8ae1bd2ffb2cf2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25491-9https://doaj.org/toc/2045-2322Abstract The use of Bt proteins in crops has revolutionized insect pest management by offering effective season-long control. However, field-evolved resistance to Bt proteins threatens their utility and durability. A recent example is field-evolved resistance to Cry1Fa and Cry1A.105 in fall armyworm (Spodoptera frugiperda). This resistance has been detected in Puerto Rico, mainland USA, and Brazil. A S. frugiperda population with suspected resistance to Cry1Fa was sampled from a maize field in Puerto Rico and used to develop a resistant lab colony. The colony demonstrated resistance to Cry1Fa and partial cross-resistance to Cry1A.105 in diet bioassays. Using genetic crosses and proteomics, we show that this resistance is due to loss-of-function mutations in the ABCC2 gene. We characterize two novel mutant alleles from Puerto Rico. We also find that these alleles are absent in a broad screen of partially resistant Brazilian populations. These findings confirm that ABCC2 is a receptor for Cry1Fa and Cry1A.105 in S. frugiperda, and lay the groundwork for genetically enabled resistance management in this species, with the caution that there may be several distinct ABCC2 resistances alleles in nature.Lex FlagelYoung Wha LeeHumphrey WanjugiShilpa SwarupAlana BrownJinling WangEdward KraftJohn GreenplateJeni SimmonsNancy AdamsYanfei WangSamuel MartinelliJeffrey A. HaasAnilkumar GowdaGraham HeadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lex Flagel Young Wha Lee Humphrey Wanjugi Shilpa Swarup Alana Brown Jinling Wang Edward Kraft John Greenplate Jeni Simmons Nancy Adams Yanfei Wang Samuel Martinelli Jeffrey A. Haas Anilkumar Gowda Graham Head Mutational disruption of the ABCC2 gene in fall armyworm, Spodoptera frugiperda, confers resistance to the Cry1Fa and Cry1A.105 insecticidal proteins
description	Abstract The use of Bt proteins in crops has revolutionized insect pest management by offering effective season-long control. However, field-evolved resistance to Bt proteins threatens their utility and durability. A recent example is field-evolved resistance to Cry1Fa and Cry1A.105 in fall armyworm (Spodoptera frugiperda). This resistance has been detected in Puerto Rico, mainland USA, and Brazil. A S. frugiperda population with suspected resistance to Cry1Fa was sampled from a maize field in Puerto Rico and used to develop a resistant lab colony. The colony demonstrated resistance to Cry1Fa and partial cross-resistance to Cry1A.105 in diet bioassays. Using genetic crosses and proteomics, we show that this resistance is due to loss-of-function mutations in the ABCC2 gene. We characterize two novel mutant alleles from Puerto Rico. We also find that these alleles are absent in a broad screen of partially resistant Brazilian populations. These findings confirm that ABCC2 is a receptor for Cry1Fa and Cry1A.105 in S. frugiperda, and lay the groundwork for genetically enabled resistance management in this species, with the caution that there may be several distinct ABCC2 resistances alleles in nature.
format	article
author	Lex Flagel Young Wha Lee Humphrey Wanjugi Shilpa Swarup Alana Brown Jinling Wang Edward Kraft John Greenplate Jeni Simmons Nancy Adams Yanfei Wang Samuel Martinelli Jeffrey A. Haas Anilkumar Gowda Graham Head
author_facet	Lex Flagel Young Wha Lee Humphrey Wanjugi Shilpa Swarup Alana Brown Jinling Wang Edward Kraft John Greenplate Jeni Simmons Nancy Adams Yanfei Wang Samuel Martinelli Jeffrey A. Haas Anilkumar Gowda Graham Head
author_sort	Lex Flagel
title	Mutational disruption of the ABCC2 gene in fall armyworm, Spodoptera frugiperda, confers resistance to the Cry1Fa and Cry1A.105 insecticidal proteins
title_short	Mutational disruption of the ABCC2 gene in fall armyworm, Spodoptera frugiperda, confers resistance to the Cry1Fa and Cry1A.105 insecticidal proteins
title_full	Mutational disruption of the ABCC2 gene in fall armyworm, Spodoptera frugiperda, confers resistance to the Cry1Fa and Cry1A.105 insecticidal proteins
title_fullStr	Mutational disruption of the ABCC2 gene in fall armyworm, Spodoptera frugiperda, confers resistance to the Cry1Fa and Cry1A.105 insecticidal proteins
title_full_unstemmed	Mutational disruption of the ABCC2 gene in fall armyworm, Spodoptera frugiperda, confers resistance to the Cry1Fa and Cry1A.105 insecticidal proteins
title_sort	mutational disruption of the abcc2 gene in fall armyworm, spodoptera frugiperda, confers resistance to the cry1fa and cry1a.105 insecticidal proteins
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1a4671474271486f96a8ae1bd2ffb2cf
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Mutational disruption of the ABCC2 gene in fall armyworm, Spodoptera frugiperda, confers resistance to the Cry1Fa and Cry1A.105 insecticidal proteins

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