Acute vaping exacerbates microbial pneumonia due to calcium (Ca2+) dysregulation.

As electronic cigarette (E-cig) use, also known as "vaping", has rapidly increased in popularity, data regarding potential pathologic effects are recently emerging. Recent associations between vaping and lung pathology have led to an increased need to scrutinize E-cigs for adverse health i...

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Autores principales:	Rui Zhang, Myles M Jones, De'Jana Parker, Ronna E Dornsife, Nathan Wymer, Rob U Onyenwoke, Vijay Sivaraman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a60db2cc20749d8868a7ac68c96f43f
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spelling	oai:doaj.org-article:1a60db2cc20749d8868a7ac68c96f43f2021-12-02T20:18:14ZAcute vaping exacerbates microbial pneumonia due to calcium (Ca2+) dysregulation.1932-620310.1371/journal.pone.0256166https://doaj.org/article/1a60db2cc20749d8868a7ac68c96f43f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256166https://doaj.org/toc/1932-6203As electronic cigarette (E-cig) use, also known as "vaping", has rapidly increased in popularity, data regarding potential pathologic effects are recently emerging. Recent associations between vaping and lung pathology have led to an increased need to scrutinize E-cigs for adverse health impacts. Our previous work (and others) has associated vaping with Ca2+-dependent cytotoxicity in cultured human airway epithelial cells. Herein, we develop a vaped e-liquid pulmonary exposure mouse model to evaluate vaping effects in vivo. Using this model, we demonstrate lung pathology through the use of preclinical measures, that is, the lung wet: dry ratio and lung histology/H&E staining. Further, we demonstrate that acute vaping increases macrophage chemotaxis, which was ascertained using flow cytometry-based techniques, and inflammatory cytokine production, via Luminex analysis, through a Ca2+-dependent mechanism. This increase in macrophage activation appears to exacerbate pulmonary pathology resulting from microbial infection. Importantly, modulating Ca2+ signaling may present a therapeutic direction for treatment against vaping-associated pulmonary inflammation.Rui ZhangMyles M JonesDe'Jana ParkerRonna E DornsifeNathan WymerRob U OnyenwokeVijay SivaramanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256166 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rui Zhang Myles M Jones De'Jana Parker Ronna E Dornsife Nathan Wymer Rob U Onyenwoke Vijay Sivaraman Acute vaping exacerbates microbial pneumonia due to calcium (Ca2+) dysregulation.
description	As electronic cigarette (E-cig) use, also known as "vaping", has rapidly increased in popularity, data regarding potential pathologic effects are recently emerging. Recent associations between vaping and lung pathology have led to an increased need to scrutinize E-cigs for adverse health impacts. Our previous work (and others) has associated vaping with Ca2+-dependent cytotoxicity in cultured human airway epithelial cells. Herein, we develop a vaped e-liquid pulmonary exposure mouse model to evaluate vaping effects in vivo. Using this model, we demonstrate lung pathology through the use of preclinical measures, that is, the lung wet: dry ratio and lung histology/H&E staining. Further, we demonstrate that acute vaping increases macrophage chemotaxis, which was ascertained using flow cytometry-based techniques, and inflammatory cytokine production, via Luminex analysis, through a Ca2+-dependent mechanism. This increase in macrophage activation appears to exacerbate pulmonary pathology resulting from microbial infection. Importantly, modulating Ca2+ signaling may present a therapeutic direction for treatment against vaping-associated pulmonary inflammation.
format	article
author	Rui Zhang Myles M Jones De'Jana Parker Ronna E Dornsife Nathan Wymer Rob U Onyenwoke Vijay Sivaraman
author_facet	Rui Zhang Myles M Jones De'Jana Parker Ronna E Dornsife Nathan Wymer Rob U Onyenwoke Vijay Sivaraman
author_sort	Rui Zhang
title	Acute vaping exacerbates microbial pneumonia due to calcium (Ca2+) dysregulation.
title_short	Acute vaping exacerbates microbial pneumonia due to calcium (Ca2+) dysregulation.
title_full	Acute vaping exacerbates microbial pneumonia due to calcium (Ca2+) dysregulation.
title_fullStr	Acute vaping exacerbates microbial pneumonia due to calcium (Ca2+) dysregulation.
title_full_unstemmed	Acute vaping exacerbates microbial pneumonia due to calcium (Ca2+) dysregulation.
title_sort	acute vaping exacerbates microbial pneumonia due to calcium (ca2+) dysregulation.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1a60db2cc20749d8868a7ac68c96f43f
work_keys_str_mv	AT ruizhang acutevapingexacerbatesmicrobialpneumoniaduetocalciumca2dysregulation AT mylesmjones acutevapingexacerbatesmicrobialpneumoniaduetocalciumca2dysregulation AT dejanaparker acutevapingexacerbatesmicrobialpneumoniaduetocalciumca2dysregulation AT ronnaedornsife acutevapingexacerbatesmicrobialpneumoniaduetocalciumca2dysregulation AT nathanwymer acutevapingexacerbatesmicrobialpneumoniaduetocalciumca2dysregulation AT robuonyenwoke acutevapingexacerbatesmicrobialpneumoniaduetocalciumca2dysregulation AT vijaysivaraman acutevapingexacerbatesmicrobialpneumoniaduetocalciumca2dysregulation
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Acute vaping exacerbates microbial pneumonia due to calcium (Ca2+) dysregulation.

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