Transgenic hepatitis B: a new model of HBV infection

Abstract Chronic hepatitis B infection (HBV) is major cause of morbidity and mortality throughout the world. Currently there is limited understanding on the cellular proteins and related molecules involved in the critical steps of viral entry into the cytoplasm and persistent viral replication in ce...

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Autores principales:	Hubert D.-J. Daniel, Michael Torbenson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a683703641e4ce6ad29c3b7eedfe2da
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spelling	oai:doaj.org-article:1a683703641e4ce6ad29c3b7eedfe2da2021-12-02T12:32:53ZTransgenic hepatitis B: a new model of HBV infection10.1038/s41598-017-02862-22045-2322https://doaj.org/article/1a683703641e4ce6ad29c3b7eedfe2da2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02862-2https://doaj.org/toc/2045-2322Abstract Chronic hepatitis B infection (HBV) is major cause of morbidity and mortality throughout the world. Currently there is limited understanding on the cellular proteins and related molecules involved in the critical steps of viral entry into the cytoplasm and persistent viral replication in cell culture. In order to address these fundamental questions, we designed and implemented a new model of hepatitis B: infectious transgenic hepatitis B virus composed of a complete virus plus a foreign gene. The foreign gene allows identification of cells that are infected by the transgenic virus. The transgenic virus was used in a functional assay to identify cellular proteins necessary for viral replication. This assay repeatedly identified the protein UQCR10. After restoring UQCR10 levels in HepG2 and Huh7 cells, they can be infected by intact virions of transgenic hepatitis B. These results demonstrate the usefulness of this new transgenic hepatitis B model.Hubert D.-J. DanielMichael TorbensonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hubert D.-J. Daniel Michael Torbenson Transgenic hepatitis B: a new model of HBV infection
description	Abstract Chronic hepatitis B infection (HBV) is major cause of morbidity and mortality throughout the world. Currently there is limited understanding on the cellular proteins and related molecules involved in the critical steps of viral entry into the cytoplasm and persistent viral replication in cell culture. In order to address these fundamental questions, we designed and implemented a new model of hepatitis B: infectious transgenic hepatitis B virus composed of a complete virus plus a foreign gene. The foreign gene allows identification of cells that are infected by the transgenic virus. The transgenic virus was used in a functional assay to identify cellular proteins necessary for viral replication. This assay repeatedly identified the protein UQCR10. After restoring UQCR10 levels in HepG2 and Huh7 cells, they can be infected by intact virions of transgenic hepatitis B. These results demonstrate the usefulness of this new transgenic hepatitis B model.
format	article
author	Hubert D.-J. Daniel Michael Torbenson
author_facet	Hubert D.-J. Daniel Michael Torbenson
author_sort	Hubert D.-J. Daniel
title	Transgenic hepatitis B: a new model of HBV infection
title_short	Transgenic hepatitis B: a new model of HBV infection
title_full	Transgenic hepatitis B: a new model of HBV infection
title_fullStr	Transgenic hepatitis B: a new model of HBV infection
title_full_unstemmed	Transgenic hepatitis B: a new model of HBV infection
title_sort	transgenic hepatitis b: a new model of hbv infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1a683703641e4ce6ad29c3b7eedfe2da
work_keys_str_mv	AT hubertdjdaniel transgenichepatitisbanewmodelofhbvinfection AT michaeltorbenson transgenichepatitisbanewmodelofhbvinfection
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Transgenic hepatitis B: a new model of HBV infection

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