Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6

Abstract The purpose of this study was to increase our knowledge about Mycobacterium africanum and report the incidence and characteristics of tuberculosis (TB) due to their lineages in Aragon, Spain, over the period 2003–2019. The study includes all the cases in our region, where all the M. tubercu...

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Autores principales:	Jessica Comín, María Luisa Monforte, Sofía Samper, Aragonese Working Group on Molecular Epidemiology of Tuberculosis (EPIMOLA), Isabel Otal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1a6bedabe22e436bb440dbdcf2ba6832
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spelling	oai:doaj.org-article:1a6bedabe22e436bb440dbdcf2ba68322021-12-02T15:43:23ZAnalysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 610.1038/s41598-021-89511-x2045-2322https://doaj.org/article/1a6bedabe22e436bb440dbdcf2ba68322021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89511-xhttps://doaj.org/toc/2045-2322Abstract The purpose of this study was to increase our knowledge about Mycobacterium africanum and report the incidence and characteristics of tuberculosis (TB) due to their lineages in Aragon, Spain, over the period 2003–2019. The study includes all the cases in our region, where all the M. tuberculosis complex isolates are systematically characterised. We detected 31 cases of M. africanum among 2598 cases of TB in the period studied. TB caused by M. africanum is rare (1.19%) in our population, and it affects mainly men of economically productive age coming from West African countries. Among the isolates, Lineage (L) 6 was more frequent than L5. The genotyping of these strains identified five clusters and 13 strains with a unique pattern. The isolates’ characterisation identified a copy of IS6110 within the moaX gene, which turned out to be specific for L6. It will allow the differentiation of this lineage from the rest of MTBC with a simple PCR reaction. It remains to be established whether this polymorphism may limit M. africanum transmission. Furthermore, a mutation in the mutT2 promoter was found as specific for L6 strains, which could be related to the high variability found for L6 compared to L5.Jessica ComínMaría Luisa MonforteSofía SamperAragonese Working Group on Molecular Epidemiology of Tuberculosis (EPIMOLA)Isabel OtalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessica Comín María Luisa Monforte Sofía Samper Aragonese Working Group on Molecular Epidemiology of Tuberculosis (EPIMOLA) Isabel Otal Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6
description	Abstract The purpose of this study was to increase our knowledge about Mycobacterium africanum and report the incidence and characteristics of tuberculosis (TB) due to their lineages in Aragon, Spain, over the period 2003–2019. The study includes all the cases in our region, where all the M. tuberculosis complex isolates are systematically characterised. We detected 31 cases of M. africanum among 2598 cases of TB in the period studied. TB caused by M. africanum is rare (1.19%) in our population, and it affects mainly men of economically productive age coming from West African countries. Among the isolates, Lineage (L) 6 was more frequent than L5. The genotyping of these strains identified five clusters and 13 strains with a unique pattern. The isolates’ characterisation identified a copy of IS6110 within the moaX gene, which turned out to be specific for L6. It will allow the differentiation of this lineage from the rest of MTBC with a simple PCR reaction. It remains to be established whether this polymorphism may limit M. africanum transmission. Furthermore, a mutation in the mutT2 promoter was found as specific for L6 strains, which could be related to the high variability found for L6 compared to L5.
format	article
author	Jessica Comín María Luisa Monforte Sofía Samper Aragonese Working Group on Molecular Epidemiology of Tuberculosis (EPIMOLA) Isabel Otal
author_facet	Jessica Comín María Luisa Monforte Sofía Samper Aragonese Working Group on Molecular Epidemiology of Tuberculosis (EPIMOLA) Isabel Otal
author_sort	Jessica Comín
title	Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6
title_short	Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6
title_full	Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6
title_fullStr	Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6
title_full_unstemmed	Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6
title_sort	analysis of mycobacterium africanum in the last 17 years in aragon identifies a specific location of is6110 in lineage 6
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1a6bedabe22e436bb440dbdcf2ba6832
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Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6

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