Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.

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Autores principales:	Su Bin Moon, Jeong Mi Lee, Jeong Gu Kang, Nan-Ee Lee, Dae-In Ha, Do Yon Kim, Sun Hee Kim, Kwangsun Yoo, Daesik Kim, Jeong-Heon Ko, Yong-Sam Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1a7208fe96764c9c9ae3002772e791ad
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spelling	oai:doaj.org-article:1a7208fe96764c9c9ae3002772e791ad2021-12-02T15:34:27ZHighly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang10.1038/s41467-018-06129-w2041-1723https://doaj.org/article/1a7208fe96764c9c9ae3002772e791ad2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06129-whttps://doaj.org/toc/2041-1723Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.Su Bin MoonJeong Mi LeeJeong Gu KangNan-Ee LeeDae-In HaDo Yon KimSun Hee KimKwangsun YooDaesik KimJeong-Heon KoYong-Sam KimNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Su Bin Moon Jeong Mi Lee Jeong Gu Kang Nan-Ee Lee Dae-In Ha Do Yon Kim Sun Hee Kim Kwangsun Yoo Daesik Kim Jeong-Heon Ko Yong-Sam Kim Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
description	Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.
format	article
author	Su Bin Moon Jeong Mi Lee Jeong Gu Kang Nan-Ee Lee Dae-In Ha Do Yon Kim Sun Hee Kim Kwangsun Yoo Daesik Kim Jeong-Heon Ko Yong-Sam Kim
author_facet	Su Bin Moon Jeong Mi Lee Jeong Gu Kang Nan-Ee Lee Dae-In Ha Do Yon Kim Sun Hee Kim Kwangsun Yoo Daesik Kim Jeong-Heon Ko Yong-Sam Kim
author_sort	Su Bin Moon
title	Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
title_short	Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
title_full	Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
title_fullStr	Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
title_full_unstemmed	Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
title_sort	highly efficient genome editing by crispr-cpf1 using crispr rna with a uridinylate-rich 3′-overhang
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1a7208fe96764c9c9ae3002772e791ad
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Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

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