Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.

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Detalles Bibliográficos
Autores principales: Su Bin Moon, Jeong Mi Lee, Jeong Gu Kang, Nan-Ee Lee, Dae-In Ha, Do Yon Kim, Sun Hee Kim, Kwangsun Yoo, Daesik Kim, Jeong-Heon Ko, Yong-Sam Kim
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/1a7208fe96764c9c9ae3002772e791ad
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